稀释

Combining chemical complexation and physics extraction of monobasic and polybasic carboxylic acid, the universal liquid-liquid equilibrium model with clear physical meaning was successfully induced.

详细研究了萃取剂浓度、稀释剂浓度、水相pH值、温度和羧酸初始浓度等因素对络合萃取平衡的影响以及萃取剂的再生条件。

Either methanol or other suitable solvent, such as ethylene glycol monomethyl ether, may be used as the diluent.

可能是甲醇或者其他合适的溶剂，例如二乙醇甲醚，可以用做稀释剂。

The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence ＋ serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁＋无血清条件培养液纯化的实验。

METHODS: NSCs were isolated from neonatal rats by enzyme digestion and mechanical separation. At the fourth passage, cell clone masses received nestin immunocytochemistry. Remaining cells were dispersed by mechanical separation. Monoclone NSCs were incubated by limiting dilution assay, and made into 108 L-1 monoplast suspension in complete medium. NSCs were assigned into 2 groups. NSCs in the control group were incubated in 10% fetal bovine serum.

酶消化和机械分离法相结合体外分离培养新生鼠神经干细胞，传至第4代的细胞克隆团行巢蛋白免疫细胞化学染色观察，剩余细胞团用机械法分散，采用有限稀释法进行单克隆神经干细胞培养，加入完全培养基制成108 L-1的单细胞悬液，分为2组滴入培养板，对照组加入10%FBS，诱导组加入10%FBS+50 μg/L神经生长因子，培养5~7 d。

METHODS: Olfactory bulb was sterilely collected from rats, digested, and centrifuged. After removal of the supernatant, the olfactory bulb was resuspended with DMEM/F-12 medium containing 0.2 volume fraction of fetal bovine serum. The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence + serum-free conditioned medium.

①无菌条件取大鼠嗅球组织，消化、离心去除上清液后，用含体积分数为0.2胎牛血清的DMEM/F-12培养基重悬，稀释的单细胞混悬液均分成3份，分别用于单纯改良差速贴壁、单纯无血清条件培养液纯化和改良差速贴壁+无血清条件培养液纯化的实验。

The greatest efficiency in Agrobacterium-mediated transformation of lettuce cotyledons(2～3 days-old) was obtained by 10-time dilution of Agrobacterium cells, 15-min inoculation time,1-day pre-culture,4-day co-culture and without AS using the MS(Murashige and Skoog\'s,1962) medium supplemented with 0.2 mg/L 6-benzylaminopurine,0.1 mg/Lα-naphthaleneacetic acid,100 mg/L Kan and 500 mg/L Carb,.

在农杆菌介导的皱叶生菜子叶(2～3 day苗龄)转化试验中，采用含BA 0.2mg/L，NAA 0.1 mg/L，Kan 100 mg/L，Carb 500 mg/L的MS培养基，最优的转化条件为农杆菌稀释倍数为10倍，预培养时间为1 day，共培养时间为4 day，浸染时间为15 min，不添加乙酰丁香酮。

Using culture medium selection, diluted separation, plate lineration, centrifugal separation, capillary separation, droplet separation, pH separation, temperature separation, inhibitor separation and so on, ten species were obtained. There were Cyanophyta Nostoc punctiforme, Phormidium tenue, an undetermined alga; Chrysophyta Chromulina ovalis; Chlorophyta Cholorella ellipsoidea, Scenedesmus bijuga, S. quadricauda, Elakatothrix sp., Selenastrum minutum and Bacillariophyta Navicula sp.

采用培养基筛选法、稀释分离法、平板划线分离法、离心分离法、毛细管分离法、小滴分离法、pH值分离法、温度分离法、抑制剂分离法等9种不同方法，最终分离纯化出4门9属10种藻类，其中包括蓝藻门念珠藻属的点形念珠藻、席藻属的小席藻和一未定种；金藻门单鞭金藻属的卵形单鞭金藻；绿藻门小球藻属的椭圆小球藻、栅藻属的双对栅藻和四尾栅藻、纺锤藻属的一种、月牙藻属的小形月牙藻和硅藻门的舟形藻属的一种。

The acute virus necrobiotic disease virusC AVND viruswas isolated from diseased scallop Chlamys farreri collected in the Taiping Bay of Qingdao. Using purified virus as antigens, the polyclonal antibody with high titer was prepared in rabbits. The polyclonal antibody as the first antibody, IgG-HRP as the second antibody, indirect ELISA technique was set up through selecting the best interactional conditions among the first antibody, the second antibody and antigen.

以患病栉孔扇贝组织中提纯的急性病毒性坏死症病毒作为免疫抗原，注射新西兰白兔制备出效价高达1：32000的多克隆抗体作为第一抗体，第二抗体为辣根过氧化物酶标记的羊抗兔IgG，通过交叉稀释法确定抗原、一抗及二抗的最优反应条件，建立酶联免疫吸附分析方法体系，病毒检测灵敏度达30ng。

Methods: The minimum concentration inhibitory to Neisseria gonorrhoeae of ceftriaxone was determined by using the agar dilution method.

采用琼脂稀释法测定头孢三嗪对淋病奈瑟菌的最小抑菌浓度(minimalinhibitoryconcentration ，MIC)。

The determination of lead content in naphtha by graphite furnace atomic absorption spec-troscopy was studied with tetrahydrofuran as solvent, nickelous nitrate as matrix modifier and suitable amount of iodine solution added in samples.

将试样用四氢呋喃稀释，加入适量碘溶液，以硝酸镍为基体改进剂，研究了用石墨炉原子吸收光谱法直接进样测定石脑油中的铅含量。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。