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Cells inosculation was carried out by standard method.The hybridoma supernatants were selected by indirect ELISA.The selected cells were subcloned three times by limiting dilution .Three hybridoma lines against TGEV rMP were obtained , which were designated as 1B3、3C2、4A5. The isotypes of the McAbs were IgG2a、IgG1、IgG1. The three hybridoma lines can secrete antibodies steadily after culturing and saving for a long time.

并以纯化的M蛋白为抗原,免疫BALB/c小鼠,按常规方法进行融合,间接ELISA方法进行筛选,采用有限稀释法,经过三次克隆,最终获得三株抗重组TGEV M蛋白的杂交瘤细胞系(命名为1B3、3C2、4A5),分泌的单克隆抗体分别为IgG2a、IgG1、IgG1类,三株杂交瘤细胞在体外长期培养和长期冻存时都稳定地分泌单克隆抗体。

Prior to the Closing, the Company will reserve shares of its Common Stock so that __% of its fully diluted capital stock following the issuance of its Series A Preferred is available for future issuances to directors, officers, employees and consultants.

交割前,公司应保留适当普通股,以便公司在将来发行 A 系列优先股后,可以为董事、高级管理人员、员工及顾问提供在全面稀释后股份总数?%的普通股。

The following issuances shall not trigger anti-dilution adjustment: reserved as employee shares described under the Company's option pool,… iii

通常,在某些特殊情况下,低价发行股份也不应该引发防稀释调整,我们称这些情况为例外事项。

EITF 07-05 requires issuers to record as liabilities financial instruments which provide "down round" protection in the form of a "hard ratchet" adjustment mechanism to the relevant exercise or conversion price, which was originally designed to protect holders from dilutive issuances of a company's stock.

EITF 07-05 要求发行者将附带有&硬棘轮&调整机制的认股权证记录为负债金融工具(以前是记录为权益金融工具),&硬棘轮&调整机制是对发行者所发行权证的转换价格提供贬值轮次保护,从而在发行人进行股票稀释时保护公司原股东的利益。

The results indicated that apple juice mingled with a certain amount of pear juice was characterized by great changes in pH, 295 nm absorbance, total sugar/reduced sugar and optical rotation, and that these 4 indexes can be used .

从相同稀释倍数下的梨汁以不同体积掺入苹果汁后的pH变化,295nm处的吸光度的变化,总糖和还原糖的变化,旋光度的变化以及糖的薄层层析和感官评定等16个方面进行了探讨。

Taking the brines from Qinghai Chaerhan salt lakes as raw material, sulfonated kerosine as diluting agent, the experiment of extracting boric acid from brines by 2 - ethyl - 1,3 - haxanediol is carried out. The concentration of extractant,acidity, the ratio of organic phase to brine, the time of extraction and extration isotherm are investigated.

以青海察尔汗盐湖提钾后老卤为原料,以磺化煤油为稀释剂,从萃取剂浓度、酸度、相比、萃取时间、萃取等温线5个方面加以试验,获得了2-乙基-1,3-己二醇从卤水中萃取提硼的最佳试验条件,并与2-乙基己醇和磺化煤油的萃取体系进行比较,得出二元醇的萃取效果高于一元醇的结论。

Good for steam, water, ketones, and diluted acids.

也适用于蒸气、水、酮,以及稀释酸。

Methods: The minimum inhibitory concentrationof Xipayiguyiy on P.gingivalis,P.intermedia and F.nucleatum was observed by agar dilution in vitro.Also,the effects of Xipayiguyinye on oral malodour challenged by cysteine was investigated on 10 volunteers in vivo.Results: It showed in this study the MIC of Xipayiguyiy for Pg and Fn was 1mg/mL,and for Pi was 0.5mg/mL.It was also found the Xipayiguyinye achieved significant effects on oral malodour challenged by cysteine.

采用琼脂稀释法,测定西帕依固龈液对牙龈卟啉单胞菌(Pg、中间普雷沃菌、具核梭杆菌的最低抑菌浓度;对10例志愿者采用自身前后对照试验,观察四种干预措施(无干预、清水、西帕依固龈液、口泰)对半胱氨酸激发性口臭的最大值水平、各时间点下降百分比及口气值的下降曲线的影响,评价西帕依固龈液防治口臭的效果。

Cinnamomea fruiting body. The malt extract agar was the basic medium used in this study. The standard MEA formula was designated as 100%, and a series of dilution of the MEA were made to culture A. cinnamomea. During the culture period, we determine the formation of fruiting body by visual and microscopic observation on A.

把标准的MEA配方视为100%,对其做一系列的稀释,用来培养樟芝,在培养的过程,发现子实体的形成,以肉眼和显微镜判断其多孔性状的生成,再以HPLC进行分析。

Lidocaine and cefazolin sodium solution 1ml each were added to 20ml of HPAG. Then the mixed gel were injected into subdermis or mascle of guinea-pigs 0.5~2ml at each point with 18 gauge needle.

实验组以2%利多卡因1ml与头孢唑啉钠溶液1ml稀释水凝胶后,用18号注射针头将其注入到豚鼠皮下或肌肉层,每个部位注射量为0.5~2ml。

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Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气,收缩臀部肌肉;拱起身体,尽量抬起头来,右腿伸直朝向天花板(膝微屈,以避免肌肉紧张)。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化,但却比去年同期高8%。

However, to get a true quote, you will need to provide detailed personal and financial information.

然而,要让一个真正的引用,你需要提供详细的个人和财务信息。