稀有

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

Mat I hold more culpable, and less politic; except it be in great and rare matters.

那我认为，除非在重大与稀有的事件之中，是罪过多于智谋的。

However, because of its rarity the most common type is the fresh water cultured ones.

然而，由于其稀有的最常见的类型是淡水养殖的。

It exerts important significance on sci-tech history of mining and smelting and historical culture research, since this mine is one of a few rare mines in China that are qualified to restore the technique scenes of "decrepitating method" mining and "cupellation method" smelting.

公园拥有诸多采矿遗址，其中黄岩坑古矿硐规模最大，保存最完整，古代文献记载最丰富详实，是国内稀有的有条件恢复"烧爆法"采矿、"灰吹法"冶炼工艺场景的矿山，对矿冶科技史和历史文化研究具有重要意义。

The growth of a wide variety of rare wildlife, such as elephants, great hornbill, bison, peacocks, curare wood, Yoshinori wood, light wood , natural wood and so on.

生长着品种繁多的稀有野生动植物，如象、大犀鸟、野牛、孔雀、箭毒木、美登木、轻木、天然木等。

The eastern curlew is the largest species; the common, or Eurasian, curlew, almost as large, is the largest European shorebird.

普通杓鹬(N.arquata，欧亚杓鹬)是欧洲最大的滨鸟；爱斯基摩杓鹬是世界稀有鸟类之一，已濒于绝灭

Cymophane effect is rarely seen in spinel.

具有猫眼和星光效应的尖晶石更是稀有的品种。

Gobiocypris rarus is an endemic cyprinid in China.

稀有鮈鲫是我国特有的一种小型鲤科鱼类。

Danburite are rare crystals of calcium boron silicate, the best of which are usually found in Mexico .

赛黄晶是一种稀有的钙硼硅酸盐晶体，世界上品质最优的赛黄晶产于墨西哥。

A team of biologists have already confirmed that the plant life is safe, but believe that the rare breeds of Daubenton's and Pipistrelle bats that live in surrounding woods could be disturbed.

一个由生物学家构成的研究小组已经对演唱会所能造成的影响进行了评估：植物不会受到危害，但是生活在附近森林里的一种稀有Daubenton's and Pipistrelle 蝙蝠有可能受到侵扰。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。