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移植生长

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Conclusion52 eyelashes in 30 patients have been treated well through this method.resultseach pelage is with complete follicle, the survival rate is high, and it can also control the direction and the density of the pelage, in order to make it looks like true eyelashes.

结果:30例52只眼睫毛缺损通过本方法治疗均取得良好疗效。结论:每根移植的毛发均带完整的毛囊,成活率高,并可控制毛发的生长方向及密度,可取得近似真睫毛的效果。

Relatively good results were also obtained similarly by using fresh autografts of periosteum and cartilage.

软骨细胞可以在筋膜上正常生长,筋膜可以作为软骨细胞移植的较为理想的载体。

Oridonin had potently inhibitory effects on the proliferation of the gastric cancer cell BGC823 in vivo and in vitro,and it reduced the expression of MMP-2 and MMP-9 of the BGC823 tumor.

本实验旨在观察Ori在体内、外对胃癌BGC823细胞生长的影响,及对BGC823移植瘤MMP-2、MMP-9表达的影响。1材料1.1药品与试剂Ori(纯度98%,北京桑浩博公司)。

Physeal sparing, combined intra-articular and extra-articular reconstruction of the anterior cruciate ligament with use of an autogenous iliotibial band graft in skeletally immature prepubescent children and adolescents provides excellent functional outcome with a low revision rate and a minimal risk of growth disturbance.

干骺端保守治疗结合关节内及关节外前十字韧带重建、自体髂胫束移植术治疗骨骼发育中的青春期前儿童及青少年能提供良好的功能恢复,且二次手术率低,并能把医源性生长紊乱的危险降到最低。

A new parallel-epiphyseal cartilaginous tissue was found in the subcutaneous tissue 4 weeks after operation. The tissue was stained with HE, toluidine blue, safranine O and collagenⅡ. A large number of chondrocytes and a cartilage lacuna-like structure were observed under a microscope with no obvious inflammatory reaction around the calcium alginate and polylysine microspheres.

结果 体外培养时微球中骺板细胞生长、增殖情况良好;移植皮下后第4周取材可见外形呈类骺软骨样组织块,行HE、甲苯胺蓝、番红"O"及Ⅱ型胶原等染色,镜下观察可见大量软骨细胞及类软骨陷窝样结构,植入的海藻酸钙微球周围组织无明显炎症反应。

Results:(1)NSCs form typical neurospheres under adequate concentration in vitro, which are immunoreactive to Vimentin. Typically and terminally differentiated mature neural cells could not be found without the stimulus of mitogen or only under NSCs self-regulation and self-induction;(2)NSCs derived from hippocampus maintain the character of stem cells much longer with better biological behavior; NSCs passed to the 2-3 passage are the best to graft since they have not differentiated;(3)NSCs cultured in vitro could self-regulate and differentiate into neurospheres and progenitors positively immunoreactive to specific antibodies representing neurons, astrocytes, oligodendrocytes and Schwann cells;(4)There are widespread synaptic contacts between various kinds of descendent clones and cells;(5)Neurospheres could be formed without the stimulus of mitogen when NSCs and OECs are cocultured. Many neurospheres and cells immunoreactive to Vimentin, GFAP, MAP2, 02, p75NGFR, GFAP, S-100, Synaptosis, Vimentin, Tau (Tau is only positive in cocultureof HNSCs+HOECs) could be found;(6)The supernatant fluid triturated from adult rat spinal cord stimulates NSCs to differentiate into neurons, but do not terminally differentiate;(7)Fibroblasts and O4 oligodendrocytes are not supported to grow under this culture medium.Part II: Isolation, culture and identification of rat and human olfactory ensheathing cellsOlfactory ensheathing cells/glials are the most powerful cells to enable the regeneration of axons in the central nervous system.

结果表明:①在适宜的浓度体外培养条件下,NSCs能形成典型的神经干细胞克隆球,Vimentin免疫荧光染色阳性,单靠丝裂原刺激或NSCs自我调节和分化诱导,不会产生典型的终末分化的成熟神经细胞;②海马源性的NSCs维持干细胞特性的时间更长,生物学特性更优;③传至第2~3代的NSCs尚未分化时移植最佳;④体外培养的NSCs能自我调控分化为神经元、星形胶质细胞、O2少突胶质细胞、雪旺氏细胞染色阳性克隆球和前体细胞;⑤各种子代克隆球和细胞存在广泛的突触联系;⑥NSCs与OECs联合培养时,不需丝裂原刺激即能形成克隆球,获得大量Vimentin、GFAP、MAP2、O2、p75NGFR、GFAP、S-100、Synaptosis、Vimentin、Tau(Tau只有人HNSCs+HOECs联合培养时出现阳染)染色阳性的克隆球和细胞;⑦脊髓研磨后的上清液刺激神经干细胞向神经元方向分化,但并不出现终末分化;⑧本研究培养条件不利于成纤维细胞、O4生长。

It is common for these trees to shed a large amount of leaves during the first growing season due to transplant shock.

它是常见的这些树木掉了大量的树叶在第一生长季因移植性休克。

Objective To observe the influence of gene transfection of inducible nitric oxide synthase to the transplanted human laryngeal squamous carcinoma in nude mice, to investigate the expression and the correlayion of iNOS and p53, and to proke into the possible apoptosis mechanism. Methods Transfect the gene of iNOS to Hep-2 human laryngeal cancer cell, sieve out positive clone cell and amplify.

目的 观察诱生型一氧化氮合酶(inducible nitric oxide synthase, iNOS)基因转染对人喉鳞癌细胞裸鼠移植瘤生长的影响,研究在体人喉鳞癌细胞中iNOS与突变型p53基因的表达,探讨二者的相关性及促进肿瘤细胞凋亡的机制。

Objective:To investigate the inhibitory effects of human angiostatin on the subcutaneously transplanted mouse glioma.

目的:观察人血管抑素(Angiostatin,AS)对小鼠脑胶质瘤皮下移植瘤生长的抑制作用。

Objective: To investigate the inhibitory effects of the Bcl-2 antisense oligodeoxynucleotides on tumor formation and growth of human lung carcinoma transplanted subcutaneously in nude mice.

目的 探讨Bcl-2反义寡核核苷酸对裸鼠人肺癌移植瘤的成瘤能力和生长的抑制作用。

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In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

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