移植生长

The thickness of myelin sheath of experimental group was inferior to that of group B (P.05) at the third month, while the number and diameter of axons in both groups was similar.

这种无细胞基膜管移植体能支持轴突的生长和雪旺细胞的迁移，是一种良好的神经移植替代材料。

OBJECTIVE: To compare the growth and development after ovary organ transplantation in different regions of mice.

目的：比较小鼠卵巢器官不同移植部位自体移植后的生长发育情况。

Objective To establish a human cervical carcinoma cell line with stable expression of green fluorescent protein and the fluorescent-labeled implanted tumor model, and compare the detection methods of tumor growth by optical imaging system and slide caliper.

目的 建立稳定表达绿色荧光蛋白的人宫颈癌细胞系，建立移植瘤模型并比较移植模型肿瘤生长的荧光分析和卡尺测量的优缺点。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

There was apparent interspace between repaired tissue and surrounding normal tissues. The results indicated that: 1 Although autogenic cartilage obtained from patella in knee joint is limited, chondrocytes cultured in vitro could be regulated by some growth factors, therefore sufficieng and well-phenotype chondrocytes could be harvested to construct tissue engineering implants for transplantation. 2 It showed well therapeutic efficacy with the method of repair in our research.

实验结果表明：①自体膝关节髌骨来源的软骨虽有限，但在体外单层培养时应用生长因子对其进行调控，能为自体关节软骨缺损的移植治疗提供数量充足的、表型良好的软骨细胞以用于构建组织工程移植物；②本实验中所应用的修复方法治疗效果良好，能较有效地克服和解决目前在临床上所用的自体关节软骨细胞移植修复关节软骨缺损时所存在的软骨细胞容易丢失等问题。

Impact of PCL capsulation on the mesenchymal stem cell activity studied in vivo, one plate culture with complete culture medium for control group with and two encapsulated transplantation for experimental groups(complete culture medium or basic culture medium for cell suspended) installed,after package and transplantation,retrieving capsulated cells for cell count,MTT assay,CCK-8 assay Daily for 8days to determine cell activity,and analysis growth curve.

以完全培养液平皿法细胞培养为对照组，设2个实验组进行聚己内酯囊管化骨髓间充质干细胞移植细胞活性研究。两个实验组分别以完全培养液和基础培养液悬浮细胞后封装移植，囊管内细胞回收后以细胞计数、MTT法、CCK-8法测定细胞的活性，共8天，进行生长曲线分析。

In A549 group and controling group 0.9% NS were injected to mice by abdomen respectively. In DDP group 1000uM cisplatine were injected to nude mice by abdomen which was inefficiency to A549DDP cells.

第二章 人参单体Rh2和顺铂抑制A549DDP细胞移植瘤生长的作用研究目的：探讨人参单体Rh2和顺铂对A549DDP细胞移植瘤的抑制作用。

Results: The survival rate of all skin grafts except for skin graft on diploic layer, which was about 90%, were 100%. Three skin flaps and 15 expansive skin flaps survived and the wounds healed on the first stage. Any complication was not found.

结果：所移植的皮片除板障层移植皮片成活率约为90%外，其余皮片均100%成活；3个皮瓣和15个扩张皮瓣均成活，伤口一期愈合，无任何并发症发生，随访显示毛发生长良好，外形满意。

Decreased expression of MMPs by blocking the expression of Snail in ovarian carcinoma cells Objective: To investigate the influence of antiadhesive antibody (SHE78-7), targeted at the potent hemophilic cell adhesion molecule E-cadherin, on the motility and invasive ability of ovarian carcinoma cells that have stably tansfectant with anti-Snail plasmid.

第二部分反义Snail对裸鼠卵巢癌原位移植瘤转移的影响目的：观察转染反义Snail质粒的卵巢癌细胞在裸鼠原位移植瘤模型中生长及转移的情况，检测转移组织中Snail、E-钙粘蛋白、及基质金属蛋白酶的表达和分布。

Objective: To optimize the culture system of hEGcs in vitro, select the hEGcs from subculture to transplant directly to the rat infarcted myocardium and observe the therapeutic mechanism of their heterotransplantation in the treatment for the rat myocardial infarction.

目的：优化人胚胎生殖细胞的体外培养体系，并将生长状态良好的传代的人EG细胞移植用于大鼠心肌梗死模型的治疗，探讨人胚胎生殖细胞进行异种移植对大鼠心肌梗死的治疗机制。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。