移植生长

Three cases of clinical congenital anophthalmos were reported. Their treatment included: silicone ball as pressure conformer initially in one case; conjunctival socket reconstruction with mucosal graft and canth oplasty in the second case; orbital implant surgery combined with mucosal graft of conjunctival socket in the third case.

本文报告三例临床型先天性无眼球症及其治疗方法：第一例先以矽球当做conformer以刺激眼窝生长；第二例以口腔黏膜移植术重建结膜窠和外眥成形术；第三例以眼窝植入物重建眼窝和口腔黏膜移植术重建结膜窠。

Objective To study the effect of taurine on the living and growth of transplanted cells in Parkinson disease rat models after fetal mesencephalon cells transplantation.

目的观察牛磺酸对帕金森病模型大鼠胚胎中脑细胞移植术后移植细胞的存活及生长的影响。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

A total of 90 healthy male Sprague Dawley rats were selected for this study. Twelve rats were used for the sham operation group. Left anterior descending coronary artery of the remaining rats was deligated to establish models of myocardial infarction. After 24 hours of deligation, 36 rats were randomly divided into the control group and experimental group, 18 rat in each group.

目的：检测人胰岛素样生长因子Ⅰ基因修饰的鼠骨骼肌成肌细胞移植后，心肌梗死大鼠血浆及心肌组织内胰岛素样生长因子Ⅰ蛋白浓度的变化，以及心肌细胞内胰岛素样生长因子Ⅰ和端粒酶反转录酶mRNA水平的表达。

Methods Fetal ovaries were transplanted to rats who had received unilateral ovarian extirpation or oophorotomy, and then the interaction between local ovaries and transplanted ovaries was detected.

将胚鼠卵巢分别移植至单侧卵巢摘除及去势雌鼠皮下，观察同时存在的自身卵巢对移植卵巢生长发育有无影响，以及自身卵巢是否受移植卵巢影响。

METHODS: After cultured and propagation, human lung adenocancer of A549 was implanted on BALB/c nude mice. Tea polyphenols, Iressa and drug combination were taken by peroral administration. Antitumor function was determined with regular methods.

人源可移植性肺腺癌（A549）细胞系经传代培养后，以BALB/c-nu祼鼠进行肿瘤移植，并以茶多酚，易瑞沙及其联合用药进行干预性治疗，通过观察抑瘤率评价单用及联合用药对A549移植瘤生长抑制作用。

Three mice were killed every time at interval, of one, two, three, four, and six weeks after the implantation, and the tissue where the composite was implanted was excised for histologic investigation of heterotopic steogenesis under photomicroscopy.

结果：大鼠骨髓基质细胞体外培养后增殖，植入后2周（1只）、3周（2只1）、4周（1只）、6周（1只），在石膏和陶瓷人工骨复合移植侧载体周边均见骨生长、骨软骨生长和骨髓生长，各阶段中均见纤维组织增生。

The experimental result shows that the main determinative factor is the temperature of the sea water for the growth after the transplantation; the optimal temperature for the growth of Zostera marina L. is 10~20℃; it is strongly salt-tolerant and it can grow at the salinity range of 20‰~48‰ normally; its rhizome is the optimal organ for culturing and the optimal hormone concentration for the calluses induction is 2mg/L or so.

实验结果表明，大叶藻移植后生长的主要制约因素是海水温度；大叶藻生长的最适温度是10～20℃；大叶藻对盐度的耐受性很强，它可以在盐度为20‰～48‰的范围内正常生长；地下茎是组织培养的最适材料；诱导愈伤组织的最适激素(2，4-D)浓度为2mg/L。

Methods: The hEGcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared.

取5~10周人胚胎生殖腺嵴和背侧肠系膜，用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养，选取生长情况良好的传至第4代的人EG细胞，移植到急性心肌梗死大鼠的梗死心肌周围，分别于移植后1天、1、2、4周处死大鼠，以鼠抗人细胞核抗体MAB1281作为示踪剂，通过免疫组化方法检测移植细胞的分布。

Methods: the hegcs from the gonadal ridges and dorsal mesenteries of human embryos aged 5-10 weeks were cultured with or without cytokine in culture medium to observe their growth and the effects of different cell culture systems were compared. the hegcs' from passage 4 were transplanted directly to infarcted myocardium.

取5~10周人胚胎生殖腺嵴和背侧肠系膜，用添加和不添加细胞因子的两种培养体系对比进行组织块原代及传代培养，选取生长情况良好的传至第4代的人eg细胞，移植到急性心肌梗死大鼠的梗死心肌周围，分别于移植后1天、1、2、4周处死大鼠，以鼠抗人细胞核抗体mab1281作为示踪剂[1]，通过免疫组化方法检测移植细胞的分布。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。