移植生长

HE staining and prussian blue staining in all transplantation mice were done and the result of the staining was analyzed. RESULTS: The macrophages were seen in the inner of 2 subcutaneous ectopia transplantation tumor models and the kupffer cells were found in the inner of 3 subcutaneous ectopia transplantation tumor models by Prussian blue staining.

结果： Prussian blue染色皮下移植瘤组有2只瘤鼠的瘤灶内见较为聚集的单核/巨噬细胞，肝脏移植瘤组中3只瘤鼠的瘤灶内见枯否细胞；肝脏移植瘤组见7个瘤灶与正常肝组织呈明显的浸润性生长。

We examined 8 cases after costal cartilage autograft in clinical observation through optics microscope, verified that the framework keeps the nature of costal cartilage and pointed out the importance of prevention of infection. Ear reconstruction can be completed in preschool age because we observed that the reconstructed ear in preschool age could grow with the normal ear in pace (7 cases). With perichondrium costal cartilage autograft could live and grow better.

临床观察8例肋软骨移植后的组织学检查，证实了耳支架成活后保持了肋软骨的生物学特性；强调了预防感染对移植耳支架的转归及治疗效果的重要性；观察到7例学龄前儿童自体肋软骨移植后再造耳廓能随年龄同步按比例生长，表明耳廓再造可在学龄前6岁时完成。

Methods 36 rabbits were made deficit in their knee joints and then they were randomly divided into autogenous osteiochondral mosaicplasty compound factor gelatum group, antogenous osteiochondral mosaicplasty group, and periosteum reimplantation group and control group.

方法将36只健康家兔膝关节造成实验性缺损，随机将动物分成自体骨软骨镶嵌移植联合凝胶生长因子复合体组、自体骨软骨镶嵌移植组、骨膜移植组和空白对照组。4、8、12周后对修复组织进行形态学观察及组织学检查。

On day 7, 15, 30 after injection, the renascent blood vessels were more in number, the KDR area density and the KDR mRNA expression in splenic tissues were higher in VEGF group than the control group, while on day 60 there were no significant differences in the area density of renascent blood vessel, the KDR area density and the KDR mRNA expression between the two groups.

外源性VEGF能够促进移植脾组织内血管生长，自体脾组织移植术后7~15 d开始静脉应用VEGF是促进血管再生的较理想时间；外源性VEGF可能是通过与移植脾组织内所表达的KDR结合发挥作用。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Finally,Tumorigenecity of KB-Neo,KB-PF4 and KBp17-70 cells wasassayed with xenograft tumor growth in isogenous nude mice by examiningthe growth speed of xenograft,survival span,and histochemistry ofxenograft nude mice.

最后，对重组的PF4和p17-70基因进行体内实验，建立裸鼠移植瘤模型，观察移植瘤的生长速度、移植瘤的体积、荷瘤鼠的生存时间、移植瘤瘤体组织中的血管密度。

There are different viewpoints about the live of costal cartilage autograt, the repair of offer region and the recovery style of the cartilage, especially about the selection of surgical time in congenital microtia, the use of chondrium in the growth of cartilage autograft and the growth ability of the reconstructed ear in preschool children.

对于自体肋软骨移植后能否成活，肋软骨膜在移植软骨中的作用，供区的修复以及软骨间的愈合方式等均存在不同的看法；特别是对先天性小耳畸形患儿的手术时机的选择，肋软骨膜在移植软骨生长中的作用以及学龄前儿童再造耳能否随年龄增长而与正常耳同步按比例生长等均存在不同的观点。

However, once rooted,"transplanted hair is normal growing hair; it can be cut, coloured, curled and will grow forever," says hair colourist Daniel Galvin OBE, who had his first transplants four years ago.

但一旦移植后的头发生根下来之后，&它们就成了正常生长的头发，可以剪切、染色、吹卷，永远生长下去，&染发师丹尼尔·高尔文说，他在四年前进行了首次头发移植手术。

Methods Tumor tissue was implanted into the fat pad of mammary gland of the nude mice, which were randomized to groups A, B and C when the tumor reached a certain size.

目的 探讨应用化学修饰的小干扰RNA沉默人裸鼠乳癌移植瘤新生血管中的血管内皮生长因子受体1（VEGFR1）基因对移植瘤生长的影响。

Following successful modeling, rats of bFGF group were intratracheally injected with 400 U bFGF and rats of VEGF group with 2 μg VEGF, once a week for three times. MSCs group was injected 1 mL suspension of 4×109/L MSCs into tail vein. MSCs+VEGF group was injected MSCs into tail vein and intratracheally injected VEGF (2 ug, three times) at the same time. Model control and normal control groups were intratracheally injected with equal volume of sodium chloride.

成功造模后，碱性成纤维细胞生长因子组气管内注入400 U碱性成纤维细胞生长因子，血管内皮生长因子组气管内注入2 μg血管内皮生长因子，1次/周，共3次；单纯细胞移植组于尾静脉注入4×109 L-1骨髓间充质干细胞悬液1 mL；血管内皮生长因子+细胞移植组气管内注入血管内皮生长因子的同时，尾静脉注入骨髓间充质干细胞；模型对照组、正常对照组给予相同体积的生理盐水。

Plunder melds and run with this jewel!

掠夺melds和运行与此宝石！

My dream is to be a crazy growing tree and extend at the edge between the city and the forest.

此刻，也许正是在通往天国的路上，我体验着这白色的晕旋。