移植于

Porcine was acted as the experimental animal. It was researched on problem of the origin of obtaining early embryonic cells, methods of separating blastomere cells, best parameter and order of ECNT embryo electric activation and system of ECNT embryo IVC, which was to find a scientific method of fitting ECNT and a procedure of fitting the lab producing embryos largely, optimize the procedure of ECNT and improve the ECNT embryo in vitro.

本实验以猪为实验动物，对猪胚胎细胞核移植供体的来源、卵裂球细胞分离方法、核移植重建胚的电激活最佳参数、核移植受体的激活时序以及核移植重建胚体外培养系统相关问题进行了研究，旨在探索一种适用于猪胚胎细胞核移植的科学方法，提高猪胚胎细胞核移植胚胎的体外发育率，优化胚胎细胞核移植程序。

Their sex proportion、pretransplant Hb、pre- and post-transplant BP、diuretic therapy、immunosuppressive drugs、native kidney nephrectomy、pre-transplant lymphotoxin cross test and prePTE S cr/BUN were recorded and analysed. The results showed that those nonAza treated hypertensive male recipients with higher pre-transplant Hb and excellent allograft function were susceptible to PTE.

分别记录其性别组成、移植前Hb、移植前后高血压发病情况、利尿剂应用、免疫抑制药、自体肾是否切除、移植后发病前SCr／BUN及移植前淋巴毒交叉试验，并与总体对照比较，发现PTE好发于移植前Hb较高、移植前后血压较高、不服用Aza而肾功能良好的男性肾移植病人。

Median time of follow-up was 44.5 months (range, 13-89 months). 1 patient died of hypertensive encephalorrhagia at 22 months and 2 patients died of relapse at 12 months and 38 months respectively after transplantation, the other 12 patients remain alive (including CR1 5 cases, CR2 2 cases, PR1 3 cases, PR2 1 case and refractory 1 case) with an OS of 75%, and an EFS of 62.5%.

全部病例随访至2006年5月，中位随访时间44.5个月（13～89个月），1例于移植后22个月死于高血压脑卒中，2例分别于移植后12个月及38个月死于复发，其余12例存活至今（其中CR1 5例，CR2 2例，PR1 3例，PR2 1例，耐药1例）总生存率75%，无事件生存率62.5%。

Methods RI and PI of main renal artery and interlobar artery were obtained by color Doppler ultrasonography within the first week of transplantation.Then they were correlated with allograft glumerular filtration rate at 1 year.

在100例无并发症肾移植患者中，应用彩色多普勒超声，于肾移植术后1周内，测量移植肾肾动脉和叶间动脉的阻力指数和搏动指数，并与术后1年的移植肾肾小球滤过率进行相关分析。

To try to limit such complications, David Pilcher, MD, a senior intensive care unit physician at Alfred Hospital in Melbourne, Australia, and colleagues developed a treatment guideline to direct care in the first 72 hours after transplant. On a simple 2-sided sheet of paper, designed to be hung at the bedside and practical enough to be useful to nurses and junior physicians, the algorithm includes direction on hemodynamic care on 1 side and respiratory care on the other.

May 3， 2007－过去的回溯性研究已经证实，接受移植病患其中央静脉压力高或是PaO2/FiO2（动脉氧气分压/吸气氧气浓度）低，在手术后，相较于没有些症状的病患，仍然需要呼吸器辅助、或是早期死亡风险较高根据项发表于国际心脏与肺脏移植学会第27届年会与科学座谈的研究，现在，研究者已经发现在手术后加护时使用一标准治疗流程，可以缩短病患仍然需要呼吸器的时间、且降低发生重移植器官功能不全的机率。

Methods: Forty adult Wistar rats were used for these operations of transplantation of frozen autogenous tooth-mandible compound and fresh ATMC. Then the healing process after transplantation of frozen ATMC and fresh ATMC was compared by clinical, radiographical, histological and microangiographical observation.

选用健康成年Wistar大白鼠40只，任取2只用于观察正常颌骨的组织学图像，其余的大鼠随机分为新鲜移植组和冷冻组，行牙-下颌骨复合组织块移植到胫骨区的手术，分别于术后1、2、3、4、6、8、12、16、20周时处死动物，采用大体观察、X线片、组织学及微血管造影等观察手段对牙-下颌骨复合组织块移植后的愈合过程进行比较。

Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

Results 1.After treated by:program freezing,the maximum load of the allografts has no evident decrease and the cell activity of allografts was preserved well,the frozen hurt after program freezing was more slight than －80℃ deepfrozen,2.No evident reject reaction can be seen during the healing process after deep frozen and the reject reaction declined as the time pass by.3.Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

It was found that in the group of allogra fts intragraft IL-2R and ICAM-1 were expressed on the POW 4 and peaked on the POW 32, and their expression levels were significantly higher than in the group of autografts and isografts.

结果发现同种异体肾移植组于第4周开始出现IL-2R及IC AM-1的表达，于32周达高峰，明显高于自体肾移植组和同质移植组。

According to correlative literatures, the diagnosis and treatment of bronchiolitis obliterans syndrome in three patients with hematological malignancies after allo-HSCT were analyzed. The function of haematopoiesis re-established successfully. The acute graft versus host disease did not occur in these patients except one patient occurred grade Ⅱ aGVHD, and the chronic graft versus host disease occurred in the 5th, 6th and 6th months after allo-HSCT respectively, but the cGVHD was recrudescent after treatment. The bronchiolitis obliterans syndrome occurred in the 9th, 7th and 11th months after allo-HSCT respectively, and they received the treatment of immunosuppressive, immune human serum globulin, azithromycin and to breathe in β2 receptor agonist and budesonide.

结合相关文献，对3例恶性血液病患者异基因造血干细胞移植后发生闭塞性细支气管炎综合征的临床特点和诊断治疗进行分析。3例患者移植后造血功能均顺利重建，2例未发生急性移植物抗宿主病，1例发生II度急性移植物抗宿主病；3例患者分别于移植后5，6，6个月发生广泛型慢性移植物抗宿主病，经治疗后病情仍有反复；3例患者分别于移植后9，7，11个月发生闭塞性细支气管炎综合征，给予免疫抑制剂、静脉免疫球蛋白、阿奇霉素及局部使用β2受体激动剂及布地奈德吸入治疗，2例在治疗4周后病情有所改善，另1例病情无改善。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。