种质

The results showed:① The new gemplasms had high nutritive value and taste quality and were similar as the protogene type in each biochemical constitution.② The heterotic rates were negative orientation on the most of quality character,but it was evidently different from the growth vigour character.③ The external...

研究结果表明，（1）甜玉米新种质各种生化成分与原始基因型接近，营养价值高，食味品质好；（2）甜玉米品质性状优势率以负优势为主，明显不同于生长势性状的优势率；（3）普甜、甜脆、超甜玉米籽粒外观性状和糖分含量均受其各自的一对隐性基因控制

The ISSR fingerprinting of 18 Japanese plum cultivars and 12 plum species or variety were established by using optimum system with primer 5"-9T-3"and 5"-9C-3", respectively. The identification rate of plum cultivars and species or variety was 100%. The effects of five important components on reaction of SSR were studied with Prunus salicina cv. Meilili. The results showed that the optimum concentration of five important components i.e. Tag DNA polymerase, Mg +, single primer, template DNA and dNTPs in 25 μL reaction system of SSR were 1.5u, 2.0mM, 0.8 μ M, 30~40ng and 0.16~0.24mM, respectively. The dendrograms of these cultivars was contructed according the Jaccard coefficient of similarity.

利用该优化体系，以5′-_9T-3′为引物，构建了中国李18个品种的ISSR指纹图谱，该引物可将这些品种完全区别开来；以5′-_9C-3′为引物，构建了李属6类种质资源的ISSR指纹图谱，该引物区分率为100％。4、中国李品种适宜的SSR反应分析体系是：25μL SSR反应体系中，Taq DNA聚合酶、Mg~(2+)、每个引物、模板DNA和dNTPs等5种成分的适宜浓度分别是：1.5u、2.0mmol·L~(-1)、0.8μmol·L~(-1)、30～40ng和0.16～0.24mmol·L~(-1)。5、采用RAPD、ISSR和SSR等三种分子标记技术对中国李品种资源54份和2个欧洲李品种进行了遗传多样性分析，并以品种间的遗传相似系数构建了Jaccard聚类树状图。

The study on the genetic diversity of 56 radish accessions was made by integrating the RAPD and AFLP data.

将RAPD标记和AFLP标记的结果整合在一起，对56份萝卜种质的遗传多样性进行了研究。

A total of 255 bands were detected among 76 squash accessions from different countries and regions.

利用这26条RAPD引物对来源于不同国家和地区的76份南瓜种质基因组DNA扩增，共扩增出255条，其中多态性带为207条，多态性百分率为81.18%。

There are about 3000 accessions of mulberry germplasm resources in China.

我国保存有桑树种质资源近3000份，其中可作果桑用的资源超过60份。

The result is identical to ACGM marks cluster analysis of 12 accessions of Signal grass.

这与用ACGM标记分析12份臂形草种质进行聚类分析的结果一致。

The cytoplasmic male-sterility was transferred into leaf mustard by inter-varietal hybridization and subsequent backcrosses using cms donor of tuber mustard.

采用榨菜细胞质雄性系为不育源，通过变种间杂交和回交的方法，转育叶用芥菜细胞质雄性不育种质。

In this study, genetic analyses were conducted to determine the genetic basis in an elite resistant inbred line Siyi with complete resistance to maize dwarf mosaic. A new genetic model, two dominant complementary genes conditioning the resistance, were found by Mendelian genetic analysis based on parents, F1, F2 and backcrosses in three successive years' field trails. The two genes were further mapped near the centromere of chromosome 3 and 6, respectively by tightly linked microsatellite markers using 242 plants from F2 generation. The resistance gene on chromosome 3 is 1.0 cM apart from the flanking markers phi053 and umc1527, respectively. Whereas the linkage distance between two flanking markers bnlg1600 and phi075 and resistance gene on chromosome 6 was 1.0 and 4.0 cM, respectively. Genotypic analysis of the plants from testcross and F3 populations supported the new genetic manners.

课题组通过连续三年的抗病鉴定，在国内种质资源中筛选出一份综合农艺性状优良、配合力较高的自交系四一，三年的表型遗传研究和两年的分子标记工作，发现四一中的玉米矮花叶病抗性是由两对显性互补基因控制的，进而利用F2作图群体，把发现的两个基因定位在第三和第六染色体的着丝点附近，并获得了双侧紧密连锁的分子标记连锁图谱，其中第三染色体上的分子标记UMC1527和phi053从抗病基因双侧逼近1 cM，而第六染色体上的分子标记phi075、bnlg1600从抗病基因双侧分别逼近4 cM和1 cM；利用B2群体、F2：3家系、BC3F1群体和带有第三、第六染色体抗病基因以及两个抗病基因的近等基因系，进一步证实了四一中成株期抗性是由两个显性互补基因控制的。

These single-copy transformants could be used for the culture of resistance-herbicide and also produce new variety through backcrossing and crossing.

这些材料可直接生产杭除草剂杂交种，也可作为抗除草剂的种质资源，通过杂交、回交等常规育种方法将抗除草剂Bar基因导入目标品种，获得具有抗除草剂特性的新品种。

The efficiency of molecular marker-assisted selection was investigated using two populations from pedigree selection and modified backcrossing pyramiding developed for the breeding purpose.

利用长江流域推广品种泗棉3号和优异纤维种质系7235为育种亲本，配置了系统育种和修饰回交聚合育种两套群体。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。