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The kinetics of reconstitution of apoacylase with either 〓 or 〓 and the inactivation of the 〓 reconstituted enzyme by 1, 10-phenanthroline has been studied by following the substrate reaction continuously in presence of the metal ion or OP respectively. Although the native 〓-containing and the 〓-reconstituted enzymes have closely similar Michaelis constants and maximal velocities, the kinetics for both the inactivation by OP and the reconstitution of the apoenzyme with the metal ions differs considerably.

虽然钴重组的氨基酰化酶与天然含锌的氨基酰化酶在催化活性上比较相近,但两种酶在去金属失活动力学机制以及去金属失活的氨基酰化酶与两种金属离子重组复活的动力学过程均存在较大的差异,说明锌在氨基酰化酶中的作用比较复杂,并不能完全由钴替代。

The fungal DNA was extracted from three natural soils with greater differences in physical anti chemical properties in North China by using the second method, and the 28S rDNA-PCR-DGGE profiles showed that the fungal DNA banes were comparatively rich, suggesting that this method could be used in fungal diversity research of North China soils.

利用后一种方法分别对3种理化性质差异较大的中国北方自然土壤样品真菌DNA进行提取并分析,表明所提取土壤基因组DNA真菌特异性PCR-DGGE图谱条带丰富,该方法可用于多种北方土壤真菌多样性研究。

RESULTS: A total of 6 bisbibenzyls were detected in four bryophytes, and the results show that the sample locations and species have an influence on the bisbibenzyl composition and the content of each bisbibenzyl.

结果:共检测出6种双联苄类化合物,并且种属和采集地点差异对该类化合物的种类和含量有不同程度的影响。

The investigation shows that the seasonal fluctuation of infective rate of the two cestodes is similar, but there is difference on their infective intensity Early...

对两种假叶目绦虫季节动态的观察表明两种绦虫的感染率动态具有一定的相似性,但在感染强度动态方面存在一定差异。

The nad4 sequences of six cestodes samples(MM11JT,TH29CS,TPW32CZ,TPW33CZ, TH45XX,TPW45) were obtained and their sequence length was 523bp.The six nad4 sequences comparison revealed that they separated into three population.

获得6个代表性样品(MM11JT,TH29CS,TPW32CZ,TPW33CZ,TH45XX,TPW45)的核苷酸序列,序列长度均为523bp,经核苷酸序列互对比较,结果显示它们存在三个种群,且其种间差异为2.1%~30.1%,比种内变异(2.2%)大。

Biologists call this kind of gradual variation over a certain geographic range a "cline" and interpret clines as strong indications that the variation is adaptive, a response to environmental differences.

生物学家将這种在特定地理区域范围内的逐渐变化称为&渐变群&,并针对渐变群解释说,它强烈地表明這种变化是适应性的,是对环境差异所作出的反应。

Pparent amino acid digestibilities and true amino acid digestibilities of six feedstuffs (barley,cooking blood meal,fermented blood meal,protein hydrolysate mixed feed,heatextruded collagen protein and local fish meal) and their mixed feeds(J1 and J2) were determined with intact and caecectomized cockerels,using Sibbaled "TME" method,and their additivities were also tested.

本研究以去盲肠鸡和正常鸡按Sibbald的&TME&方法分别测定了皮大麦、蒸煮血粉、发酵血粉、水解复合蛋白、热喷胶原蛋白、国产鱼粉及其配合饲料J1和J2的氨基酸表观消化率和真消化率。并对两种试鸡所测上述饲料原料氨基酸的消化率及其可加性作比较。结果表明:(1)6种饲料原料的某些氨基酸表观消化率和真消化率,去盲肠鸡和正常鸡测值间存在显著差异(P<0.05)。(2)去盲肠鸡内源氨基酸排出量显著高于正常鸡(P<0.05)。

There was significant different in protein image among five feedstuffs and the method of Tricine-SDS-PACE ought to be the effective method in identification of the protein in feedstuff.

结论]5种饲料之间蛋白成像差异显著、直观,Tricine-SDS-PACE可以作为饲料蛋白品质鉴定的一种有效方法。

Results:Significant differences in energy and 26 kinds of nutrients such as protein,lipid,carbohydrate,calcium,zinc,selenium,magnesium,copper,manganese,natrium,kalium,iron,vitamin A,vitamin B1,vitamin B2,vitamin C,vitamin PP,vitamin E,and eight kinds of essential amino-acids were shown between recommend dietary allowance and daily food intake(P.01).The intake of iron and vitamin E was enough,whereas the intake of energy and other nutrients was not enough.

结果:热能和26种营养素,如蛋白质、脂肪、碳水化合物、钙、锌、硒、镁、铜、锰、钠、钾、铁、维生素A、维生素B1、维生素B2、维生素C、维生素PP、维生素E和8种必需氨基酸(异亮氨酸、亮氨酸、赖氨酸、蛋氨酸、苯丙氨酸、苏氨酸、色氨酸、缬氨酸)的摄入量与每人每天营养素标准供给量比较,均有显著性差异(P.01),其中只有铁和维生素E摄入尚足。

Numerical analysis indicated that B2817 was a species of genus Vibrio and shared a high homology with V. gazogenes. However, there were obvious differences between them in phenotypic and chemotaxonomic characterization. The result from this study indicates that B2817 is probably a novel species of Vibrio.

经多相分类学鉴定,发现B2817是弧菌属中一个种,与弧菌属中的产气弧菌Vibrio gazogenes亲缘关系最近,但两者在形态、生理生化特征、分子遗传等方面存在很大差异,故认为B2817可能是弧菌属的一株未被认识的新种。

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