种差异

Oligodendrogliomas provide a unique model to study the molecular basis of chemoresistance, as there are two distinct genetic subtypes with significant differences in chemosensitivity.

少突胶质细胞瘤提供了一种独一无二的模型来研究药物抵抗的分子基础，因为其中存在了两种在药物敏感性方面有显著差异的独特基因亚型。

Nutritional components in edible part of common Hemistepta lyrata, Cirsium setosum, Salvia plebeian, Patrinia villosa, Trigonotis peduncularis Benth were analyzed systematically. Nutritional components in 5 kinds of wild vegetables had greater difference, with ash content of 1.61%~2.97%, crude fiber content of 1.23%~2.21% crude fat content of 0.40%~1.00%, Vitamin C content of 0.09%~0.19%, potassium content of 5040.43~8463.48 mg/kg, Na content of 331.59~2835.97 mg/kg, Ca content of 1983.64~3 985.59 mg/kg and Fe content of 6.24~122.07 mg/kg.

对常见的泥糊菜、蓟菜、雪见草、苦菜和附地菜可食用部分的营养成分进行了系统分析。5种野菜的营养成分差异较大，灰分含量为1.61%~2.97%，粗纤维含量为1.23%~2.21%，粗脂肪含量为0.40%~1.00%，Vc含量为0.09%~0.19%，钾含量为5040.43~8463.48 mg/kg，钠含量为331.59~2835.97 mg/kg，钙含量为1983.64~3985.59 mg/kg，铁含量为6.24~l22.07 mg/kg.5种野菜的有害元素硝酸盐含量较低，为0.0020~20.0088 g/100 g，完全符合安全食用标准。

The zymogramatic variations of esterase isoenzyme and superoxide dismutase isoenzyme of 20 sample materials, including 5 species, 2 varieties, 1 multiploid of Isatis L. and 1 outgroup species, Coronopus didymus J. E. Smith, were analyzed with technique of polyacrylamide gel electrophoresis.

采用聚丙烯酰胺凝胶电泳，比较了菘蓝属5种2变种1多倍体品种及1外类群种共计20个样本的酯酶同工酶和超氧化物歧化酶同工酶的酶谱差异，并运用数量分类学的原理和方法对酶谱数据进行了聚类分析。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

In this study, on the basis of the grain size analysis using the laser diffract instrument and sieve-pipette methods for several model sediments, the two sets of grain size parameters are compared and the differences between the two methods are analyzed.

为了解激光粒度仪法和传统的筛析－沉降法在模型沙粒度测量方面的差异，用这两种方法对几种模型沙样品分别进行了粒度分析。

The diluent contain magnesium chloride, potassium citrate, sodium glutamate, dipotassium hydrogen phosphate, monopotassium phosphate and fructose, semen vitality is hightest after frozen.The average value is 0.52,highter than other 5 diluent significantly (P﹤0.01).So chose this diluent as semen cryopreservation diluent on chicken. Definite cryoprotectant, equilibrium time and thaw temperature:On the base of test one,research freeze sheltering effect about glycerin, DMSO ,DMA and DMF, each with 4 consistency.The result showed that 10%DMSO sheltering effect is the best, the next is 4%DMA,semen vitality are 0.58 and 0.55 after frozen.

冷冻保护剂、平衡时间和解冻温度的确定在试验一的基础上，研究甘油、二甲基亚砜、二甲基乙酰胺、二甲基甲酰胺4种冷冻保护剂在4种浓度下对鸡精子的冷冻保护作用，结果表明，10%DMSO保护效果最好，其次为4%浓度的DMA，精液冻后活力分别为0.58和0.55，两者之间差异不显著(P﹥0.05)。

I found that the major distinction for gene duplicability in protein complexity is between hetero-complexes, each of which includes at least two different types of subunits, and homo-complexes, which include monomers and complexes that consist of only subunits of one polypeptide type.

结果发现，基因可复制性在异复合体（由两种以上的次单元所构成的复合体，hetero-complexes）及同复合体（单体或是单一种次单元所构成的复合体，homo-complexes）之间存在明显的差异。

Simmer. Results: Immunohistochemistry showed that the temporal and spatial expression patterns of AMELX, AMBN and ENAM in ameloblasts and enamel matrix were different. With the mineralization of enamel matrix, expression of AMELX decreased, but it weakly expressed in adjacent bone.

结果：免疫组化结果显示，三种蛋白在三种小鼠牙胚的成釉细胞、釉质、牙本质中的表达存在时空顺序差异，随着牙胚发育和釉质成熟，AMELX呈递减表达，而AMBN和ENAM则较恒定表达。

In two different models the bonding strength has no significant difference between Gluma comfort bond and Single bond.

Gluma comfort bond和Single bond分别在两种建模方式下进行比较，两种粘结剂粘结强度的差异无显著性意义。

The heterogenic C57BL/6J splenocytes disappeared at 1 day after transfusion, but the syngeneic BALB/c splenocytes could survive for a long time in the peripheral blood of BALB/c mice. The fluorescence intensity was not decreased in two kinds of splenocytes. The results demonstrated that CFSE-labeling with difference concentration is reliable, stable and convenient for spleen lymphocytes tracing in vivo.

异基因的C57BL/6J脾细胞在输注1 d后就快速消失，而同基因BALB/c脾细胞能够在BALB/c小鼠外周血中长期存在，但是两种细胞荧光强度均未见降低，说明荧光染料CFSE差异浓度标记活细胞进行体内示踪是一种稳定的示踪方法。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！