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In all 6 species, 5S rDNA was located at the paracentromeric region of a pair of middle-sized telocentric chromosome.

结果表明5S rDNA在6种石斑鱼染色体组中,均位于1对中等大小端部着丝粒染色体且靠近着丝点的位置上,种间和种内未观察到差异,在染色体上的分布模式基本一致。

AFLP marker technique was used to investigate the genetic relationships between 50 accessions of maize and its wild relatives including teosinte, Tripsacum and Coix With twenty one AFLP primer combinations of PstⅠ/MseⅠ, 641 AFLP amplified bands were obtained, including 586 polymorphic bands with an average of 30 52 per primer combination and the polymorphism percentage of 91 4% Cluster analysis using UPGMA method based on the data of AFLP showed that the 50 materials could be divided into...

用AFLP分子标记技术对 5 0个有代表性的玉米及其野生近缘种大刍草、摩擦禾、薏苡材料进行了遗传关系分析。利用 2 1个PstⅠ MseⅠ引物组合检出可辨认带共 6 4 1条,其中多态性带 5 86条,平均每对引物得到 30 5 2条多态性带,多态性 91 4 %。聚类分析结果表明,可以将 5 0个材料分为 3大类,与已有的玉米族分类一致,但与玉蜀黍属内的种间和种内分类存在差异,说明大刍草各种内存在较大的遗传多样性。本实验发现玉蜀黍属与摩擦禾关系比与薏苡的关系更近,但并没有发现玉米与小颖玉米亚种比与其他大刍草的遗传关系更近。

The results showed that the crude protein content,the eighteen essential amino acids and the twelve essential amino acids in the powder of tetraploidy black locust for fodder leaves were highly at the four stages,and along with the time process,they were all descending gradually.Moreover,their essential amino acid score were also highly and their amino acid were balanceable.And their essential amino acid index of cattle,pig,chicken,duck,goose,fish,prawn and sheep were higher than 0.86,therefore they can be the excellent fodder of these animals.

结果表明,饲料型四倍体刺槐叶粉4个时期的粗蛋白1、8种氨基酸总量和12种必需氨基酸含量均较高,且呈现随时间的推移而逐渐下降的趋势;其必需氨基酸分均较高,氨基酸组成较平衡;对牛、猪、鸡、鸭、鹅、草鱼、对虾和羊8种动物的必需氨基酸指数4个时期之间无差异,均大于0.86,可作为这些动物的优良植物饲料蛋白源;在7月中下旬其生物量和营养达到了最佳结合点,适宜采摘或刈割。

The avenue planting of nine cities in Zhejiang has the following characters: 1. Avenue planting tree species are plenty; 2. Avenue planting benefits among the cities are not uniform and tree species used are various; 3. Tree species used for avenue planting are simple and landscape effects unideal; 4. Cultivated manner and stratal structure are simple, ecological benefits no good; 5. The tree species ratio, of evergreen with deciduous, of fast-growing with slow-growing, is out of proportion, and short-term...

通过对浙江省具代表性的9个主要城市行道绿化状况的调查分析,结果表明浙江省城市行道绿化具以下几个特点:①行道树种资源丰富;②城市之间绿化效果差异悬殊,所采用的绿化树种各有侧重;③绿化树种单一,色彩单调,景观效果差;④栽植配置方式单一,层次结构简单,生态效益差;⑤常绿树种与落叶树种、速生树种与慢生树种比例失调,近期效益考虑多,远期效益考虑少。

The content of mineral elements in ripe fruits of M.sieversii from 78 seedings at Mohe Town,Gongliu Country were analyzed using Atomic Absorption Spectrophotometer.The results indicated that M.sieversii seedings presented considerable genetic variations in content of mineral elements with the range of variation coefficients from 24.2% to 54.0%.So it has large potential for further selection.Four elite seedlings were selected,namely large fruit type,high Ca content type,high Zn content type and high damascenone content.As the predominant component of Ily wild fruit tree forest,Malus sieversii had occupied an area of 9300ha.But the inherent reproduction system of M.sieversii and genetic diversity of the species were destructed in different degrees,as a result of farming assart,over grazing and damage by Agrilus mali.

上述结果支持&新疆野苹果可能是栽培苹果祖先种&的结论;②对巩留县莫舍乡的新疆野苹果78个实生株系果肉组织Ca、Mg、Fe、Zn、Cu、Mn6种矿质营养元素进行测定的结果表明,每种矿质元素含量单株间差异显著,变异系数在24.2%~54.0%,遗传多样性丰富,进一步选择的潜力很大;③根据新疆野苹果果实形态、香味物质和矿质元素含量等的测定结果,初步筛选出了大果型、高钙型、高锌型和大马酮型等4个特异性状单株;④调查发现,由于农田开垦、过度放牧以及苹果小吉丁虫的蔓延危害,目前伊犁野果林面积逐年减少,野苹果固有的繁育体系和遗传多样性受到不同程度的破坏。

Results The UV-B treatment caused the declines of chlorophyll content, soluble protein content and water content of leaves, besides, dose-effect was existed. The decline of 30 μW/cm^2 treatment was bigger than that of 15 μW/cm^2 treatment. The influences of UV-B radiation on carotenoid content and anthocyanidin content were similar; the change trends were declined firstly then increased. The UV-B treatment with two doses restrained the electron transport of PSⅡ, particularly; the inhibitory effect was biggest after treated 2h, and then this effect was declined in 4, 6 and 8 h, so the dose-effect was existed. The UV-B radiation with two doses restricted photosynthetic rate and the inhibitory effect increased with the increase of treatment time. The high dose treatment caused huge damage to membrane system, while the result of low dose treatment was not obvious.

结果]UV-B处理导致叶绿素、可溶性蛋白含量以及含水量都降低,且存在剂量效应,30μW/平方公分处理的降幅大于15μW/平方公分;UV-B辐射处理对类胡萝卜素含量的影响与花青素相同,均表现为先下降后上升的趋势,2种剂量间差异不大;2种剂量的UV-B处理均抑制了PSⅡ的电子传递,尤其是处理2 h的抑制作用最大,4、6和8 h的抑制作用有所缓解,且存在剂量效应;2种剂量的UV-B辐射均抑制了叶片的光合速率,随着照射时间的延长抑制效应加大;高剂量UV-B处理对膜系统的损伤较大,低剂量UV-B处理结果不明显。

The application of ultrasonic method in bored concrete pile detection;2. Suo′s method and ultrasonic method for extracting BPBenzo(aPyrene content from coke oven gas were introduced.

阐述了对焦炉煤气中苯并芘含量提取处理方法中的索氏法和超声法的两种方法,分析了两种方法测定相同含浓度苯并芘含量样品测试数据差异及产生原因,用实验数据分析说明了使用超声法作为测定焦炉煤气中苯并芘含量是一种最佳的处理方法。

The results showed that the microbial community could degrade 39.6% of rice straw gross weight within five days. When the culture medium volume was 1/5 of the whole and pH at 6 on the 5 days culture, the CMC reached the highest of 14 IUml^(-1). During the rice straw degradation, more than ten kinds of products were detected using GCMS. DGGE detected the dynamic change of microbial community composition, and the microbial composition changed greatly in different periods. The phylogenetic tree derived from 16s rDNA sequence was found that the closest relatives belong to Clostridium sp., Brevibacillus sp., Bartonella sp.、Bacteroidetes sp.

5d后稻秆总减重量达到39.6%,在培养基占总体积1/5、pH=6、培养第5天时纤维素内切酶活性表现最高,达到14IUml^(-1);培养过程中发酵液中有10余种挥发性产物,且不同时期产物的种类和浓度变化很大,从DGGE图谱发现在培养不同时期菌种组成有很大差异,通过各条带近缘种16s rDNA扩增信息构建系统树可见,各条带近缘种分别归属Clostridium、Brevibacillus、Bartonella、Bacteroidetes4个属。

Mushroom bodies and antennal lobes in Oxycetonia jucunda were conspicuous. Calyces and lobes of the mushroom bodies.were much developed. In contrast, calyces of Ambrostoma quadriimpressum and Henosepilachna vigintioctomaculata were extremely undeveloped. However, the postretinal fibres and circumpharyngeal nerves of Ambrostoma quadriimpressum were highly developed. In the three beetles, 5-HT immunoreactivity was present in all neuropils of the brain and the suboesophageal ganglion. The pattern of 5-HT immunoreactivity and the localization of immunoreactive somata which often clustered into groups were similar among these beetles, while the immunoreactivity intensity was distinct, especially in the lamina.

结果显示,分类地位相近的三种甲虫脑结构大小相差悬殊:小青花金龟脑结构相对发达,蕈形体结构显著,具有发达的冠和叶,嗅叶发达;马铃薯瓢虫和榆紫叶甲的冠极度退化,嗅叶相对不发达;但榆紫叶甲具有十分发达的网膜后纤维和围咽神经。5-HT阳性纤维在三种甲虫的所有神经纤维网中几乎都有分布,且反应模式相似,但各神经纤维网特别是神经节层染色强度差异显著。5-HT阳性胞体有成群分布现象,且在三种甲虫脑中的空间分布位置基本一致。

The results showed that at the same concentrations, the total amount of test PAHs adsorbed by the K. candel and A. marina leaf litters had no significant difference, in spite of their decomposition status. The adsorbed amount of the PAHs tended to increase with their increasing concentrations. The litters had a higher adsorption rate of or exhibited selective adsorption to phenathrene and chrysene when the PAHs concentrations were lower, and had a higher adsorption rate of benzo perylene when the PAHs concentrations were higher.

结果显示:在同等PAHs浓度下,秋茄和白骨壤落叶碎屑对4种PAHs的吸附总量相近,树种间或分解状态间的吸附差异不显著;随着PAHs浓度的增加,2种红树植物落叶碎屑对4种PAHs的吸附率均呈现逐渐增加的趋势;PAHs浓度较低时,落叶碎屑对和菲有较高的吸附率或选择性吸附,PAHs浓度较高时,落叶碎屑对苯并芘吸附率提高。

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