种差异

The average concentrations of Cr, Cd and Pb in ectoskeleton, liver, and muscle were 4.03, 0.12 and 12.31 μgg^(-1), 3.88, 1.04 and 9.12 μgg^(-1), and 4.16, 0.09 and 0.72 μgg^(-1), respectively. Among the three test sites, there existed significant differences in the enrichment of Cr in ectoskeleton and muscle, and of Cr, Cd and Pb in liver. The enrichment of test heavy metals had no notable correlation with the body mass of P. clarkia, and no significant difference was found in the enrichment of the heavy metals between female and male individuals.

外骨骼中Cr、Cd、Pb的平均含量分别为4.03、0.12和12.31μgg^(-1)，合肥市、肥西、肥东三地之间除Cr的富集量差异极显著外，其他2种重金属的富集量差异都不显著；肝脏中Cr、Cd、Pb的平均含量分别为3.88、1.04和9.12μgg^(-1)，3个地区重金属的富集量的差异都极其显著；肌肉中Cr、Cd、Pb的平均含量分别为4.16、0.09和0.72μgg^(-1)，3个地区除重金属Cr的富集量差异极显著外，Cd和Pb的差异都不显著。3种重金属污染物的富集量与体质量没有明显的相关性。3种重金属在雌雄个体之间的富集差异不显著。

The comparisons of biochemistry between OP and OR rats The differences between OP and OR rats included not only body weight, but lipids metabolism and insulin sensitivity as well, characterized with insulin resistance, increasing in serum free fatty acids and ketone body, and hepatic TC and TG in OP rats. However, no significant differences were observed in serum TG, TC, LDL, HDL and fasting glucose between OP and OR rats.⑶Comparisons of metabolites in serum, urine and liver tissue between OP and OR rats①There were significant differences in amino acids concentration between OP and OR rats,especially in liver tissue, such as high concentrations in ketogenic and glucogenic amino acids in OP rats, suggesting differences in amino acids metabolism;② The different metabolites between OP and OR rats included increasing of various saturated fatty acids and decreasing of polyunsaturated fatty acids in OP rats;③The urinary metabolites analysis indicated that different structure or metabolism of gut microflora might exist between the two phenotypes, which probably influenced the regulation of body weight gain;④The end-products of catecholamines in urine and intermediates of krebs cycle in serum in OP rats were all up-regulated, suggesting that the activity of sympatheic nervous system and energy metabolism was higher in OP rats than OR rats.

胰岛素耐受实验和胰岛素敏感指数表明OP动物的胰岛素敏感性较OR动物下降，而OP大鼠血清中游离脂肪酸、酮体、肝脏总胆固醇和甘油三酯水平显著升高；但是，OP与OR大鼠血清中总胆固醇、甘油三酯、低密度脂蛋白、高密度脂蛋白和空腹血糖等的水平并无显著性差异；⑶肥胖易感与肥胖抵抗大鼠血清、尿液和肝脏组织提取物中代谢物的比较研究表明：①OP与OR大鼠的血清、尿液和肝组织提取物中多种氨基酸的含量存在显著差异，并以肝组织中的差异氨基酸数量为最多，包括各种生酮和生糖氨基酸水平在OP组的升高，说明氨基酸代谢的差异是两种体重表型大鼠之间存在的重要差异特征之一；②OP与OR动物肝脏和血清差异代谢物中包含多种饱和长链脂肪酸的升高如十四烷酸、十六烷酸、硬脂酸等和多不饱和脂肪酸的下降如亚油酸和花生四烯酸，说明两种体重表型动物的肝脏脂肪酸代谢存在明显差异；③长期高脂饮食喂养后，动物的尿液代谢物分析表明OP与OR动物体内的肠道菌群结构存在差异，这些菌群上的差别可能在动物体重增长的调节上产生影响；④与OR动物相比，OP动物尿液代谢物中儿茶酚胺类递质的代谢终产物如高香草酸、扁桃酸和4-羟基苯乙酸明显升高。

Leaper said that studies that used this measure of talkativeness tended to find less difference between men and women than studies that looked at how much time people spent talking.

里波说，有两种调查，第一种就是采取上述健谈衡量标准的调查，着眼点在于每天究竟使用多少词汇，而第二种调查的着眼点则在于每天究竟用多少时间说话。在探讨男女之间差异的时候，以这两种调查相比，就会有这样的倾向，即前者调查出的差异，比后者调查出的差异要少。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C.

论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

Nutrition solution culture, ICP-AES, the differential centrifugation technique and sequential chemical extraction method, suppression subtractive hybridization , RAPD were used in this thesis to study the mechanisms of copper tolerance and accumulation by Commelina communis grown in copper mine deposit which was reported to be a Cu hyperaccumulator, compared with C. communis grown in normal soil. Several aspects were examined, including: difference of copper tolerance and accumulation between C. communis grown in copper mine deposit and that grown in normal soil; difference of growth and nutrient accumunation between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; physiological differences between between C. communis grown in copper mine deposit and that grown in normal soil under copper treatment; subcellular distribution and chemical forms of Cu in C. communis; identification of cDNA fragments differentially expressed in C. communis grown in copper mine deposit; genetic diversity and copper tolerance of C. communis, tolerance mechanisms to copper in C. communis are discussed.

中文题名鸭跖草对铜的耐性机理和富集特征研究副题名外文题名 Mechanisms of Cu tolerance and accumulation by Commelina communis 论文作者廖斌导师蓝崇钰束文圣学科专业环境科学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数128页关键词重金属污染植物修复超富集植物土壤污染铜污染鸭跖草馆藏号BSLW /2003 /X53 /12 论文以本实验室报道的生长于矿山上的铜超富集植物鸭跖草和生长于正常土壤中的鸭跖草为试验材料，分别运用溶液培养法、ICP-AES元素测定技术、差速离心法和化学试剂逐步提取法、抑制消减杂交技术、RAPD技术研究了矿山和非矿山两种生境鸭跖草对铜的耐性和吸收积累的差异，两种生境鸭跖草在铜胁迫下养分吸收和积累的差异，两种生境鸭跖草在铜胁迫下生理反应的差异，以及铜在鸭跖草细胞内的分布及结合形态研究，矿山鸭跖草差异表达的cDNA克隆，铜对鸭跖草种群遗传分化的影响，从这几个方面探讨了矿山鸭跖草对铜的耐性机理。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P＞0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P＜0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P＜0.05),while the difference between control group and blank group was not significantP＞0.05The difference of alkaline phosphatase activities among three groups was not significant(P＞0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP＜0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P＜0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P＞0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后，其各时间点CCK-8法检测值与空白对照无显著差异(P＞0.05)，成骨细胞均在第5天达到增殖高峰期；培养7天后，实验组细胞蛋白含量高于对照组及空白组(P＜0.05)，后两者之间则无显著差异P＞0.05碱性磷酸酶活性在三组间均无显著差异(P＞0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解，第4周时更为明显，纳米珍珠层粉较之微米珍珠层粉降解更快，而空白对照组骨洞阴影仍可见，至8周时，则所有组骨洞均己闭合修复，X-ray下已不可见原钻孔痕迹，恢复正常骨质密度；硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快，空白组速度最慢P＜0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快；由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好，无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快，至植入术后24周，实验组骨缺损区接近正常骨密度，对照组骨缺损区密度较低，空白组则呈现骨不连状态；骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P＜0.05，24周实验组的骨密度值与术前所测得的正常值无显著性差异P＞0.05动物取材大体所见均显示组织相容性良好，骨组织逐渐由植入材料两端向中央生长；常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组，至术后24周，实验组骨髓腔与材料已呈相交通状；硬组织磨片荧光显微镜下观察，两组材料在术后8周处于骨生长最快速时期，16周时速度开始减慢，术后4、8、16周时实验组的新骨生长速度均较对照组的快

Based upon the ecoanatomical method, leaf anatomical characters of eleven populations of six evergreen species (Cyclobalanopsis glauca, C. myrsinaefolia, C. gracilis, Castanopsis sclerophylla, C. eyrei and Lithocarpus glaber) from the eastern subtropical area of China were analyzed and compared with SPSS, DCA and TWINSPAN. The results are: 1. Although the leaf structures of the eleven populations still belong to the mesophyll type, they all express the trend of xerophilization. 2. There are some differences in leaf anatomical characters among populations, species and genera, which occur as the results of their adaptation to different habitats. 3. Different leaf anatomical characters have different variations among populations, species and genera, of which leaf thickness and palisade tissue thickness (considered as ecological adaptation characters) have greater variation than sponge tissue thickness, lower cuticle thickness and lower epidermis thickness (as systematical evolution characters). 4. The leaves of C. sclerophylla and C. glauca with thicker lamina, palisade tissue and cuticle (a multilayered upper epidermis in C. sclerophylla) are thought to better adapt to their dry habitat. The leaves of C. eyrei with thicker sponge tissue, although characterized by a multlayered upper epidermis, adapt to their humid and warm habitat. The leaves of C. myrsinaefolia and C. gracilis characterized by a thinner lamina, a less palisade tissue and a moderate cuticle thickness adapt to the humid and cool habitat in a middle mountain.

选取亚热带东部替代分布明显的壳斗科6种常绿树种（青冈、小叶青冈、细叶青冈、苦槠、甜槠和石栎）的11个种群叶片进行生态解剖，并用SPSS， DCA和TWINSPAN比较分析，结果表明：①尽管6种11个种群叶的结构仍属中生叶型，但有着不同程度的趋旱适应；②叶的解剖性状在属间、种间和种群间均存在着不同程度的差异，这是它们适应各自生境的结果，青冈和石栎的种内差异较大，反映出它们对环境变化有较强响应能力；③叶片的不同解剖性状在属、种类和种群间的变化并不同步，其中叶片和栅栏组织厚度等性状差异突出，反映它们更易受环境的饰变，是生态适应性状，而海绵组织、下角质膜，特别是下表皮厚度等性状差异较小，显示出这些性状的稳定性，可以认为是系统演化性状；④苦槠、青冈具有较厚的叶片、栅栏组织、角质膜和发达输导和机械组织，苦槠还具有复表皮，表现出对旱化生境的适应；甜槠尽管具有复表皮和较厚的叶片，但这是海绵组织增厚的结果，是对中生生境的适应；小叶青冈和细叶青冈具有较薄的叶片和栅栏组织、角质膜厚度居中等特点，可能是对中山凉湿生境的适应。

In order to understand these differences on gene transcript level, even to get the genes and their expression related to cell culture, we researched the differences of total mRNA of these tissues by RAPD-PCR, the result showed that the difference between embryo and head of nymphae was much closer than embyo with others, which was accorded to the time order of insect upgrown, and the tissue which is more different from embryo on transcript level ismore diiBcult to be cultured in vitro.

四种组织的培养难易程度和分离出的细胞形态的不同，说明存在着组织表达的差异，为了从转录水平上来认识它们的差异，甚而获得与细胞培养难易相关的基因及其表达产物，本实验还采用了RAPD分析的方法进行了这四种组织：中后期虫卵胚胎、若虫头部组织、成虫的精巢和卵巢的mRNA表达差异研究，结果表明：从发育方向上看，四种组织的表达差异有分化越大差异系数越大的趋势，而在相同条件下则越难培养。

Both the inducers and inhibitors of CYP3A could affect the formation of pyrrolic metabolites, therefore may affect the clivorine-induced hepatotoxicity. The difference of clivorine- induced hepatotoxicity in racial and sex can be partly attributed to this metabolic difference in racial and sex. This phenomenon is worth to be notice.

这种代谢的种属差异是其在豚鼠、大鼠及人体内毒性的差异的原因，同时Clivorine在大鼠体内还存在着代谢的性别差异，其在大鼠体内毒性的性别差异可能与此有关，这种代谢的种属和性别差异导致毒性差异的现象值得引起重视。

The compatibility of both Gvirens and B.firmus was researched. The fermentation filtrate of Gvirens , under the concentration of 70%, had not distinct inhibitory activity to B.firmus, the biomass of B.firmus would decrease with above the 70% filtrate of Gvirens, but the fermentation filtrate of B.firmus had not inhibitive role to Gvirens. 7, The cooperation control of both Gvirens and B.firm to the pine needle blight was explored:(1) Pathogen inoculation happened prior to the bio-control treatment for 7 days, however, the disease index and control effect had not distinct differences in any treatments even if combination of the two antagonists, but the susceptibility for different kind of pine were different.(2)when antagonist and pathogen were inoculated at the same time, some control effect to the pine needle blight, including certain extent cooperation control of both Gvirens and B.firm were proved, but it wasn't distinct when two antagonists used separately, and the change of the antagonist concentration had little effect to the control effect.

G.virens与B.firmus对松赤枯病的协同控制显示：(1)预先接种病菌后(7天)再进行生物防治处理，不管那种情况，感病指数和防治效果在处理间差异不明显，浓度影响无显著差异，即使是两种生防制剂联合作用也未显著提高其防效，但不同树种间的感病性有明显差异；(2)领抗菌与病原菌同时接种，对松赤枯病有一定防治效果，两菌联合有一定的协同作用，两菌分别单独施用防效差异不显著，浓度变化对防效影响不大；(3)预先接种颇抗菌能显著提高防治效果，两菌联合处理防效更优，感病树种的感病指数可降至13一15，抗病树种的感病指数可控制在3%左右；在同一浓度下B.f1'r功US、叹叮厂即s间无显著差异，在同一领抗菌下，浓度愈高，防效愈高：松，(4)三种生防接种方式的平均效果分析表明，叹F行ens最高平均防效49.5%黑 B。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。