种外进化

Mutation rate at exon of GH in five bovine species was very low with the percentage of 3.48%. The majority of nucleotide substitution was nonsense mutation and only one missense mutation was observed. Molecule tree based on haplotypes of bGH at exon 5 showed that differentiation was apparent relatively between Bubalus bubalis and Bos taurus, Bos indicus, Bos grunniens, Bos frontalis. There was no apparent differentiation among other four bovine species and they shared mutual ancestral sequence.

序列分析表明，5个牛种GH基因外显子5的遗传变异水平较低，平均核苷酸变异率约为3.48%，而且绝大多数位点的核苷酸替换是同义突变，仅发现1个错义突变位点；从GH基因外显子5序列单倍型构建的分子进化树来看，水牛与普通牛、瘤牛、牦牛及大额牛的分化相对比较明显，其他4个牛种之间并无明显分化，还享有共同的祖先序列。

Pollcn morphology of 11 Heracleum species was examined under LM andSEM. It is shown that pollens of these specics are all of retangular type andequatorial constricted type, with polar views of 3-splitted round, obtuse triangle ortrianglc. Interspecific variations exhibit in pollen size and exine exolamelle.

对独活属11种植物的花粉进行光镜和扫描电镜观察发现，本属花粉为伞形科中比较进化的矩形型和赤道收缩型；极面观3裂圆形、钝三角形、三角形；花粉大小及外壁纹饰在种间存在明显差异。

In addition, the phylogenetic relationship among several genera of Portunidea was discussed using Eriochier sinensis as the out-group.

另外，以中华绒螯蟹为外群探讨了梭子蟹科几个属种的系统进化关系。

Results Same conservative sites and key catalytic sites existed among SjLDH and LDHs from other species. Similarity of SjLDH compared to CsLDH, TvLDH and HsLDH was 75%, 17%, 58%-60% respectively. Phylogenetic analysis demonstrated that the evolution relation between SjLDH and DmLDH was closer than the relation between SjLDH and CeLDH, the relationship between SjLDH and HsLDH-B,-C was closer than HsLDH-A. Three trans-membrane regions were found, the region of 98aa-106aa in three hydrophilia regions located outside of membrane was inferred as the major antigen epitope. This antigen epitope had significant difference with LDHs from protozoon （ Pf ., Tg ., Tv .） and had 1-3 amino acid residue difference with MmLDH, HsLDH-A,-B,-C, and was the same with CsLDH. One of the key catalytic residues and substrate binding loop were located in this region. Tertiary structure demonstrated that 98aa-106aa was on the surface of the protein and formed a substrate binding loop, other two key catalytic sites were at the position near the loop.

结果 SjLDH与其他物种的同源序列含相同的保守位点及催化活性位点，与华支睾吸虫LDH同源性最高为75％，与阴道毛滴虫LDH同源性最低为17%，与人LDH（HsLDH-A，-B，-C）的同源性为58%~60％； SjLDH与果蝇的进化距离较秀丽隐杆线虫为近，3种人LDH中与HsLDH-B、-C的进化距离较HsLDH-A为近；该蛋白具有3个跨膜区域，3个高亲水性区域，主要的线性表位98aa~106aa位于膜外，与原虫LDH相同区域差异显著，而与其他LDH有1~3个氨基酸的差异，关键催化位点之一及底物丙酮酸结合区域位于该区域，蛋白质同源模建分析表明该区域位于蛋白表面形成环状结构，3个关键催化位点位于该区域或在其附近。

Yellow-throated marten\'s relationship is between Stone marten and Fisher marten.Fisher marten is the first species who was divided from Martes.The second one were Yellow-throated marten and Stone marten.Based on the analysis of the fossil note,Martes came from Central Europe,Fisher marten,Yellow-throated marten and Stone marten belonged to a branch which evolved to temperate zone.Boreal forest martens was another branch which evolved to cold environment.It is suggested that Yellow-throated marten and Fisher martenwere ascertained genus separately.4、By contrasting and analyzing 11 complete mitochondrial control region gene sequences of Mustelidae,the extended termination associated sequence domain、the central domain and the conserved sequence block domain were identified.A extended termination associated sequence(ETAS1) and eight conserved sequence(CSB-F、E、D、C、B、1、2、3) was indicated,and sequence mode was presented.Different kind of short repeat sequences were found between CSB1 and CSB2.With wolf as the outgroup,the phylogenetic evolution relationship of Mustelidae was analyzed using the neighbor-joining method.

而基于鼬亚科动物线粒体DNA12种重链编码蛋白基因序列及石貂和渔貂线粒体ND2和cyt b全序列，以狼、狗獾和水獭为外类群，应用NJ、MP和ML法构分别构建貂属和貂熊的系统进化树，结果表明：紫貂与日本貂亲缘关系较美洲貂近，石貂与真貂亚属亲缘关系较近，黄喉貂介于石貂和渔貂之间；渔貂是最早从貂属分化出的种类，其次是黄喉貂和石貂；结合化石记录分析，貂属起源于中欧地区，渔貂、黄喉貂和石貂属于一个向温带进化的分支，而真貂亚属为另外一个向寒冷环境进化的分支，建议将黄喉貂和渔貂分别定为单独的属。4、比对并分析11种鼬科动物线粒体DNA控制区序列，识别出延长终止序列区、中央区和保守序列区3个区域，指出了一个终止相关序列ETAS1及8个保守序列(CSB-F、E、D、C、B、1、2和3)，并给出了序列通式，在CSB1和CSB2之间发现具有不同形式的短串联重复序列。

Pollen morphology of 5 species in genus Tillandsia were observed by standard electronic modules. The results revealed the common features in their shape: i.e l-colporate and reticulate exine. All these showed that genus Tillandsia plants were more advanced in evolution than the other species within the same taxa.

在扫描电子显微镜下观察了5种铁兰属植物花粉的微形态，结果表明：铁兰属植物花粉的微形态具有一定的共性，即都具有1个萌发沟，花粉外壁都具网纹纹饰，说明铁兰属植物是自然类群中较进化的类群。

The partial sequences of exon Ⅰ of hormone-sensitive lipase genes in yak, cattle, zebu, and buffalo were analyzed. Comparisons of these sequences and the deduced amino acid sequences with the homologous HSL gene and protein sequences in other mammalian species including pig, human, mouse, and rat retrieved from the GenBank were carried out and finally a phylogenetic tree was constructed using the partial DNA sequences of the HSL genes in all species.

在对牛科中4种动物即牦牛、瘤牛、普通牛和水牛HSL基因外显子Ⅰ部分核苷酸序列进行测定的基础上，与Gen- Bank中其他物种相应基因核苷酸序列、氨基酸序列进行了比对分析，并构建了牦牛与其他物种间分子系统进化树。

Because of sharing characteristics with both Galliform groups, Tragopans are classified as partridges by some authors and pheasants by some others. In this study we set out to sequence the cytochrome b-gene of 19 species pheasants. and took the sequence for those 22 species from GenBank. We amplified 828 base pairs using the PCR technique. Primers designed by ourselves were based upon Galliform sequence data. Neighbor-joining method and Minimum evolution method were used to analyse cytochrome b sequences with Anhima cornuta and Kachuga dhongoka as outgroups.

通过PCR扩增鸡形目Galliformes黄腹角雉Tragopan caboti等10个属19个个体的线粒体DNA细胞色素b的部分基因，获取片段长度为828bP，以及从GenBank获取22种样本的相应序列，以角叫鸭Anhima cornuta和海龟Kachuga dhongoka为外群，分别用邻接法(Neighbor-joining， NJ)和最小进化法(Minimum-evolution， ME)，对鸡形目和将雉科的15个属分为5个属及雉族和鹑族构建分子系统树，NJ和ME系统树中，都是角雉与雉类相聚。

There are no distinct differences between ancestral character state and descendant character state in some characters. 4. Only one outgroup can't give enough information to tell what the ancestor looks like. It make us not to understand how a lot of charactors evolved. 5. The matsutake group is polyphyletic. 6. The character coding is not precise enough. 7. Some characters are not homology. The result of molecular systematic study on the matstuake group shows the group is not monophyletic. The matsutake group is more closed to Tricholoma than Armillaria. ITS sequence of Catathelasma ventricosum is nearly identical to that of the matsutake group, although apparently they are very different from each other in morphological characters.

对松口蘑群通过形态学特征进行系统发育关系分析造成很大的干扰的原因有：松口蘑的种类大多形态特征比较简单，可提供进行分析的特征不多；某些性状在一些状态之间的反复转化，造成分析上的误差；在进化过程中某些性状无明显的祖征和衍征，在分析过程中很难确定进化极性；选择外群时应该用一个特定类群进行整体理解后，确定各个性状的极性，不要只用单种作为外群；松口蘑群是一个多系群；进行比较的性状不一定是同源的；数量性状编码过于粗放，反映不出真实的情况。

In this study, the genomic structure and regulatory elements of ApoD from 10 representive species including protozoan, invertebrate, protochordate and vertebrate were analyzed and compared. The genomic structure of ApoD is less conserved in organisms from protostome to deuterostome invertebrates, while it is highly conserved among chordates including amphioxus and verebrates. All four conserved cysteine residues are present in amino acid sequence of deuterostome ApoDs, while there are only two cysteine residues in amino acid sequence of protostomes ApoD. Structure divergence between protostome and deuterostome ApoD proteins suggests their function difference. The majority of regulatory elements are present in nearly all organism ApoD genes ranging from unicellular protozoan to mammals, suggesting that ApoD plays a very fundamental role, and possesses a conserved regulatory mechanism. However, there also exist some specific regulatory elements, which are present only in certain species and may perform some special roles.ApoD mRNA expression in murine NIH/3T3 fibroblasts exposed to various stresses such as as hydrogen peroxide and UV light shows dose-dependent increase. And a fly homolog of ApoD, Glial Lazarillo, whose overexpression results in increased resistance to hyperoxia as well as a extension of lifespan under normoxia and resistance to starvation without altering lipid or protein content.

本文首先从生物信息学角度对分属于原生动物、无脊椎动物、头索动物和脊椎动物类群的10种动物ApoD的基因结构及调控区的调控元件进行分析及比较，发现：(1)ApoD基因外显子-内含子结构从原生动物草履虫到原口动物再到后口动物海胆的进化过程中不保守，但在分析的几种脊椎动物中相当保守；(2)文昌鱼ApoD基因扮演从无脊椎动物到脊椎动物承上启下的角色，可能代表了脊椎动物ApoD基因原型；(3)四个半胱氨酸保守位点在后口动物中都存在，而在原口动物中只存在两个，原口、后口动物ApoD蛋白一级结构上的差异反映蛋白功能上可能也存在一定差别；(4)调控区大多数主要调控元件为不同动物共有，说明ApoD主要功能及其表达调控在进化中相当保守；(5)ApoD基因个别调控元件是随着物种进化而出现并开始发挥相关作用，如SF-1；还有一些调控元件在进化过程中还没有发现其规律，这说明ApoD某些功能和基因表达调控模式可能因物种不同而存在一定的差异。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了