种内的

In the majority of cases, multiple clones from individuals resolved as monophyletic, which suggests that different copies of ITS not completely synchronized evolution, the same species of plant palm plants ITS sequences, sometimes far greater than differences between species of palm plants, However, the high levels of homoplasy in the ITS dataset, led to concerns that topologies obtained from these data might be unreliable; 2 Determination of the original 11 categories of Chinese palm plants matK sequences, and comparison with other 8 Chinese palm matK sequence from the NCBI GenBank database.

结果表明：部分棕榈科植物ITS序列种内差异较大，提示其不同ITS拷贝间没有完全实现同步进化，同种棕榈科植物植物ITS序列差异，有时候大于非同种棕榈植物间差异，这种情况下，由ITS序列构建的棕榈科植物系统树可能是不可信的； 2测定了11种中国原生棕榈科植物matK序列，并通过NCBI网站对GenBank数据库检索得到了其它8种中国原生棕榈科植物matK序列。

Club foot, or congenital talipes equinovarus, is a deformity of the ankle and foot that is present at birth.

马蹄内翻足，或先天性马蹄内翻足，是一种畸形的踝关节及足部这是目前在出生时。

The utility model discloses a magnetic lift device which comprises a main magnetic lift part, a rotating pole bracket, a transmission supporting saddle, a left rotating pole and a right rotating pole. A turning plate yoke device is arranged on the upper surface of the main magnetic lift part, and the turning plate yoke comprises a yoke head, a yoke head base, a constringent spring, a pulling rope as well as a rope sling. The yoke head is arranged slidingly in the hollow cavity of the yoke head base, and the constringent spring is arranged at one end inside the yoke head. One end of the pulling rope is fixedly connected with the inner end of the yoke head, while the other end is fixedly connected with the rotating pole. The rope sling is sleeved outside the pulling rope. A yoke platform matching with the lower surface of the front end of the yoke head is arranged at the tail end of a back support foot.

本实用新型公开了一种起重磁吊装置，包括磁吊主体、转杆支架、传动支座、左右转杆，在磁吊主体的上平面上还设置有翻板卡位装置，翻板卡位装置包括卡位头、卡位头座、压缩弹簧、拉索和索套；卡位头滑动设置于卡位头座内的中空腔体内，卡位头内端一侧设置有压缩弹簧；拉索的一端与卡位头内端固定连接，另一端与转杆固定连接；索套套设于拉索之外；后支脚尾端设置有与卡位头前端下平面相匹配的卡位平台；翻板转轴上套设有扭簧。

The development and metastasis process of orthotopic tumor models was observed directly by fluorescence microscope,and the size of the hypodermal tumor was measured by vernier.

眼内肿瘤生长情况用荧光显微镜直接观察，皮下肿瘤大小用游标卡尺测量；采用免疫组织化学方法对肿瘤内13种基因的表达进行检测。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An.

目的 ①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异，阐明微小按蚊复合体亲缘种种间和种内变异；②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系，以确立微小按蚊亲缘种变异的种系地位；③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

OBJECTIVE ①To compare the sequences of the ribosomal rDNA-ITS2, 28S-D3 and mitochondrial mtDNA-COII in the different origins of Anopheles minimus for detecting the intra-and inter-specific variation of the sibling species of the Minimus Complex;②To reconstruct phylogenetic trees of the Minimus Complex and Myzomyia Series for determining the position of An. minimus A and C;③To establish specific methods for differentiation of two sibling species, An. minimus A and An. minimus C, of the Minimus Complex.

中文题名微小按蚊种群变异与分子鉴别研究副题名外文题名论文作者周水森导师汤林华研究员学科专业分子流行病学研究领域\研究方向学位级别博士学位授予单位中国预防医学科学院学位授予日期2002 论文页码总数98页关键词蚊微小按蚊馆藏号BSLW /2003 /R384 /3 目的①比较微小按蚊核糖体rDNA-ITS2、28S-D3基因和线粒体mtDNA-COII基因的序列差异，阐明微小按蚊复合体亲缘种种间和种内变异；②根据基因变异重建微小按蚊复合体及迈蚊系成员的种系发生关系，以确立微小按蚊亲缘种变异的种系地位；③建立微小按蚊复合体亲缘种A和C的分子鉴别方法。

Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出，等位基因平均值、观察杂合度、Fis值、Fit值皆较高，表明非洲菊等位基因较丰富，杂合基因偏多，且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium；PP8、na、Ⅰ、h、Gi及Fst皆表明，ISSR检测遗传多样性的能力最强，其次是RAPD，等位酶最低；根据遗传距离进行聚类，三种方法都把70个品种分成A、B、C、D、E五个大组，每一组中除少数品种发生位移外，大部份品种分类结果相似，且与形态分析结果有相似性，但在每一组中，品种间的聚类差别较大，表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同；Mantel检测表明，三种标记的分析结果有显著相关性，表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异；非洲菊为组培苗，三种标记的检测结果皆表明，混合样品可以作为个体样品的代表，对整个居群的遗传多样性进行评价；这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

Lack of interspecific mtDNA RFLP,namely the close genetic similarity of these four fish species,is possibly resulted from the sympatric speciation and their short divergence time.

结果表明，这四种鲤鱼在种内和种间均缺乏mtDNA RFLP。这种现象在鱼类种间的mtDNA的RFLP研究中是罕见的。

Insert the ureteric catheter number F3 or F4 in to urocyst,cut the strictural part in radiation with cold knife until the normal posterior urethral membrane musoca and move forward the urethrascope scabbard into urocyst.43 patient...

4 3例应用冷刀切开 1～ 2次，术后 1w拔除导尿管，全部病例能自行排尿。用腔内泌尿外科内切镜治疗尿道狭窄，患者痛苦少，恢复快，是一种理想的手术方法。

Points out a tentative plan to storage kerosene with inner floating roof tank and gives experience calculation formulae of "big breathing, breathing" losses in vertical dome roof tank and inner floating roof oil tank.

提出了用内浮顶罐装煤油的设想，分别给出了立式拱顶罐和内浮顶油罐&大、小呼吸&损耗经验计算公式，并对内浮顶罐和拱顶罐装煤油时的蒸发损耗量以及两种油罐的造价等进行了对比计算，认为用内浮顶罐储存煤油是经济、合理和安全的，建议新建煤油储罐时应采用浮顶油罐。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。