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It was seen that strain 6055 generated three kinds of dextransucrases whose molecular weights were 151 kDa,142kDa and 117kDa,respectively.Among them,the former two ones were found both in supematants and cell wall,while the later was only found in the fermentation liquor.Strain DM 1-2 synthesized two kinds of dextransucrases,and their molecular weights were 183kDa and 142kDa,respectively.The former existed both in cell wall and out of cell,but the later only was located on cell surface.Strain PC 13 produced three kinds of dextransucrases,and their molecular weights were 148kDa, 138kDa and 115kDa,respectively.The former two ones were ectoenzyme while the later was linked to cell.Resembled to strain 6055,strain L4 produced two types of dextransucrases coexisting in supernatants and cell wall with molecular weights of 145 kDa and 136kDa,respectively,but the dextransucrase of 115kDa was only found in free-cells supernatants.

结果显示,菌株6055可产生分子量分别为151kDa、142kDa和117kDa的三葡聚糖蔗糖酶,其中前两者同时存在于上清液中和细胞表面,而后者只存在于上清液当中;菌株DM1-2可以产生两葡聚糖蔗糖酶,分子量分别为183kDa和142kDa,前者同时存在于上清液中和细胞表面,而后者仅能存在于细胞表面;菌株PC13可以产生分子量分别为148 kDa、138 kDa和115kDa的三葡聚糖蔗糖酶,前两者属于胞外酶,而后者与细胞相连;与菌株6055相似,菌株L4可以产生两同时存在于上清液和细胞表面的葡聚糖蔗糖酶,分子量分别为145 kDa和136kDa,而分子量为115kDa的葡聚糖蔗糖酶只存在于上清液中。

Objective:A multiresidue method based on solid-phase extraction and gas chromatography with electron capture detector was developed for the simultaneous determination of 15 pesticides in drinking water.

目的:建立同时检测饮用水中15农药残留的固相萃取-气相色谱分析方法,所检测的农药包括5有机磷、5有机氯、5拟除虫菊酯。

This research is to discuss the different effects dominant legs and non-dominant legs cause on static balance, ground reaction and speed by the following seven evaluation methods of dominant legs: ball-kick, step-up, hop-for-distance, step-forward, balance-recovery, fencing in epee, and individual preference.

摘要本研究的目的在利用七惯用脚评定方式(踢球、登阶、跳远、往前踩步、恢复平衡、锐剑防卫和个人喜好等七),分别探讨惯用脚和非惯用脚在静态平衡能力、对地反作用力和速度之差异性,并发现六评定方式的信度和七惯用脚评定方式相互之间的差异性。

The seed is close globose, seed coat divides 3; Episperm yellow, the flesh is qualitative; In seed coat white, bone; Inside seed coat is gules, flimsy is qualitative, endosperm flesh is qualitative.

子近球形,皮分3层;外皮黄色,肉质;中皮白色,骨质;内皮红色,薄纸质,胚乳肉质。

Thus the dialogueal teaching should be a human-oriented democratic and equalitarian teaching, which is characterized by communication, cooperation, acceptance and creation.

与此相适应,对话式教学应该是一民主的、平等的教学;是一沟通的、合作的教学;是一生成的、创造的教学;更是一以人为目的的教学。

Phase velocity error caused by position error of microtremor observation array is computed under different conditions such as two different dispersive phase velocity models of Rayleigh wave, three kinds of radius, 19 kinds of observation position mode and 6 types of position error.

为研究地脉动观测台阵中观测点之间的定位误差带来的影响,计算了在两瑞利波频散模型、3不同的圆周半径以及19观测位置等多情况下由6定位误差引起的瑞利波相速度相对误差,结果表明:由于圆周上观测点数目有限,在不存在定位误差时,相速度误差也是存在的,随观测点位置的不同,相速度相对误差存在差异,其最大值约为最小值的3倍。

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上,开展了如下未见文献报道的创新性的研究工作:(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究,建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法,与以前的报道相比,灵敏度和分析时间均有很大改善;(2)使用荧光素钠作为背景荧光试剂,建立了同时分析测定六黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法,扩大了激光诱导荧光检测的应用范围,对难衍生化合物的分离分析提供了一新思路和新途径;(3)以荧光素钠作为背景荧光试剂,建立了一用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法;(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物,为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一新思路。

The screen is set up in the installation box and surrounded by four mirrors. The video image, reflected by the mirrors within the box, creates a series of throw-back visual effect. It aims to create factious and repeatedly feelings, disorder, vision illusion, and confused phenomenon.

装置部分是将影片镶崁在一个盒型的镜子空间之中,四面是镜子在盒子中,反射中间的影片内容,造成一连续、镜射的视觉效果,试图制造出一虚实的感觉,及重复重叠的错乱感,让人产生一视觉错乱,一乱象。

Development period of the parasitoid pupa from the 5th instar larva fed on MSB was significantly longer. Length of cocoon of MSB treatment was significantly shorter than the control. However, no obvious differences were detected in the development periods of egg to larva, cocoon mass size, wasp emergence rate, sex ratio, longevity and forewing length of wasp between KMD1 and the control. 3. Amino acid content in the haemolymph of SSB after fed with transgenic rice Although there were no changes on the components of amino acids in the haemolymph of SSB after fed with two transgenic rice lines, there were some changes on relative contents of amino acids.

取食KMD1 36h后0天,20氨基酸中除天门冬酰胺、丝氨酸、苏氨酸和甲硫氨酸外,其它16氨基酸的含量都高于对照,其中组氨酸、甘氨酸、苯丙氨酸、异亮氨酸、赖氨酸差异达显著水平,谷氨酰胺、脯氨酸差异达极显著水平,与氨基酸总量表现一致的变化趋势;取食KMD1后1天,除天门冬酰胺、谷氨酰胺和赖氨酸高于对照外,其它17氨基酸均较对照低,但仅丝氨酸、苏氨酸和色氨酸与对照差异显著;取食KMD1后3天,绝大多数氨基酸含量的变化与取食KMD1后0天的趋势一致,除丙氨酸和色氨酸比对照稍低,其它18氨基酸均高于对照,其中精氨酸、缬氨酸差异显著,丝氨酸差异达极显著。

A physical property, such as mass, length, time, or a combination thereof, regarded as a fundamental measure or as one of a set of fundamental measures of a physical quantity

量纲一物理性质,例如质量、长度、时间或其它一组合,看成为一基本的测量或一物理量的一组基本的测量数据之一

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力