秋水仙

The apical buds of Celosia cristata were used as the explants for the rapid propagation to compare the mutagenic effects treated with colchicines at different concentrations and time duration in tissue culture.

以鸡冠花顶芽为外植体，研究其离体快繁程序并在无菌体繁殖条件下探索了秋水仙素溶液不同浓度和时间处理对鸡冠花的诱变效应。

Eamamined by fluorescin staining and Giemsa staining, 0.2 mg/L colcemid was considered suitable for inducing high percentage of micronuclei in A9 (neo12) cells, without causing death of a mass of cells.

通过荧光染色和吉姆萨染色分析，结果表明，A9(neo12)细胞经0.2 mg/L秋水仙素酰胺处理48 h后，89﹪的细胞产生微核化，每个细胞平均形成10个微核。

The results indicated that the highest tetraploid induction rate could reach 23.4% when the leaves precultured on the optimal organogenetic MS medium for 12d and treated with 10 mg/L colchione on the double layer media for 24h in vitro.

结果表明，预培养12d的兰考泡桐叶片在秋水仙素浓度为10mg/L的双层培养基上预培养处理24h时，四倍体诱导率最高达到23.4%。

The activity of glutamate-pyruvate transaminase andglutamic oxalacetic transaminase was measured using AU5400 automaticbiochemical analyzer; the activity of the hyaluronic acid,the laminin,precollagen typeⅢ type, collagen typeⅣ was evaluated according toradioimmunoassay method; the activity of transforming growth factor beta-1(TGFβ1)and tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) was measured according to enzyme linked immunosorbent assay. Results First, the effect of Baoganning on CCL_4 induced hepatic fibrosis in rats.

研究结果一、保肝宁对四氯化碳(carbon tetrachloride CCL_4)复合因素致大鼠肝纤维化模型的作用： 1、与正常组动物血清相比，模型组动物血清中的ALT、AST、HA、LN、PCⅢ、CⅣ、TGFβ1、TIMP-1显著升高(P＜0.05)；与模型组相比，保肝宁高、中、低剂量组血清中的ALT、AST、HA、LN、PCⅢ、CⅣ、TGFβ1、TIMP-1显著降低(P＜0.05)；秋水仙碱组、复方鳖甲软肝组动物血清中上述指标呈不同程度的下降趋势。

Feasibility of tricocyst test with Tetrahymena pyriformis were evaluated by serial chemicals (Cu2+, Cr2O2-4,Cd2+,MMC, CP, Colchicine). There is no significant difference between control and treated groups with negative chemical (Cu2+).

应用致突变阴性、阳性对照(Cu2+， Cr2O2-4， Cd2+，丝裂霉素MMC，环磷酰胺CP，秋水仙素Colchicine)评价四膜虫刺泡发射实验方法的可行性，并应用该方法检测稀土金属离子La3+，Ce3+， Sm3+， Y3+， Gd3+对四膜虫刺泡发射的影响效应。

Perenial flowering planrs of Strelitzia reginae are used as material. The First meiosis young flowers are induced with different concentration colchicines (0.05%, 0.10%, 0.20%).

以多年生开花鹤望兰植株为试验材料，采用不同浓度秋水仙素(0.05%， 0.10%， 0.20%)对鹤望兰处于减数分裂Ⅰ期的幼小花蕾进行诱导。

Conclusions1 Capsule FuYuanYuGan shows definite protective and therapeutic effect on experimental hepatic fibrosis model rat.2 Capsule FuYuanYuGan had no liver toxication.

湖北中医学院肝病专业硕士研究生毕业论文结论：1复元愈肝胶囊具有优于秋水仙碱的抗肝纤维化的作用。2复元愈肝胶囊无明显肝毒副作用。

The improved L.D.Cua method was used to induce tetraploid of Trichosanthes kirilowii Maxim. The suitable treating time of 0.2% colchicine, the morphology characters and cytology identification between plants of diploid and obtained tetraploid were studied.

采用改良L.D.Cua法诱导短把栝楼获得四倍体，研究了0.2%浓度的秋水仙碱诱导短把栝楼染色体倍性变异的最佳处理时间、四倍体栝楼与原栝楼的形态特征及其四倍体与二倍体的细胞学鉴定。

The DNA relative content of large unreduced pollen grains induced by colchicine was determined quantitatively with laser scanning confocal microscopy using the normal-sized pollen grains as control. The results suggest that the large unreduced pollen grains are diploid.

以正常花粉作对照，用激光扫描共聚焦显微镜对经秋水仙碱处理诱导出的大花粉进行了DNA含量的定量测定，初步确认该试验诱导的大花粉是一种二倍体花粉。

Meanwhile, by either artificial chromosome doubling via colchicine treatment or spontaneous chromosome doubling via a union of unreduced gametes (2n) from T. turgidum-Ae.

同时，通过秋水仙碱对四倍体小麦-节节麦杂种进行染色体人工加倍或利用未减数配子自然加倍，我国科学家创制了一些新的人工合成小麦。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。