秋水仙

EZ was we akly positive in CM effects test but it may not induce aneuploid in mammalian ce lls.

益康唑对细胞有丝分裂有与秋水仙素相类似的效尖，进一步分析表明其在哺乳动物体细胞内无非整倍体诱发效应。

Moreover,20 to 35% of the 2n pollens induced byColchicine developed anomalously,which also affected the effects of triploid breeding.

此外，秋水仙诱导产生的2n花粉中，还有20~35%发育表现异常，也会对三倍体得率产生较大的影响。

The results showed that the best effect of autotetraploid was obtained with 2.0 gL-1 colchicine solution for 6 times, and the frequency was 4.5%.

结果表明：经2.0 gL^(-1)秋水仙素处理6次获得同源四倍体的效果最佳，四倍体诱导率达到4.5%。

The autotetraploid plant of sesame may be effectively induced by immersing seeds with 0.3%-0.5%colchicine water solution under 28 ℃ for 24 hours.

在28 ℃的条件下，用0.3%～0.5%的秋水仙素水溶液浸种24 h，诱发产生芝麻同源四倍体的效果较好。

Colchicine is a remedy derived from autumn crocus that may be helpful for chronic back pain caused by herniated discs.

秋水仙碱是从秋番红花，可用于慢性背部由椎间盘突出引起的疼痛有帮助得出的补救措施。

Autumn crocus is the herb from which the drug colchicine was originally isolated.

秋番红花是由该药物秋水仙碱原来孤立的药草。

OBJECTIVE: To establish a HPCE method for determining the content of colchicines in Colchicine Tablets, Cremastraappendicutat Makino and Pleionebulbocodioides Rolfe , and to identify Cremastraappendicutat Makino and Pleionebulbocodioides Rolfe Pleione.

目的：建立以高效毛细管电泳法测定秋水仙碱片、毛慈姑、冰球子中秋水仙碱含量的方法，并对冰球子、毛慈姑进行鉴别。

Tetraploid induction from double haploid was also studied, and a desirable result has been obtained. Dripping the apical point of plantlets with 0.2% colchicine plus 1.5% DMSO was a suitable treatment with doubling frequency 18.2%.

其中0.2％秋水仙素+1.5％DMSO滴苗处理，效果较明显，加倍频率达18.2％。1％秋水仙素+羊毛脂膏+1.5％DMSO涂抹生长点试验，加倍率为14.0％，由于此法田间工作量小，不失为双单倍体诱导纯合四倍体较好的方法。

Sowe selected the colchicine optimum parameters were 0.6μg/ml and 6 hour culturedduration.The second, there were no significantly differences between the four groupsin 2 hour groups; and there were significantly differences between 0.2μg/ml groups and other three groups in 4 hour groups; and there were no significantlydifferences between the four groups in 6 hour groups; and there were significantlydifferences between 0.4μg/ml groups and other three groups in 8 hour groups onthe metaphases (P＜0.05), in the experiment of the effect on the mouse 8-cellembryo stage single blastomere of colchicine with different concentrations andduration by x~2 statistical analysis.

根据实验的实际情况，适宜制备小鼠4-细胞期胚胎单卵裂球染色体标本的秋水仙素的处理浓度和时间是0.6μg／ml和6小时。2、在确定秋水仙素不同处理浓度和时间对小鼠8-细胞期胚胎单卵裂球中期分裂相数影响的实验中，经统计分析，阻断培养2小时组中四个浓度组差异均不显著；阻断培养4小时组中0.2μg／ml浓度组与其他三组差异显著；阻断培养6小时组中四个浓度组差异均不显著；阻断培养8小时组中0.4μg／ml浓度组与其他三组差异显著(P＜0.05)。

The sixth, there were no significantly differences between colchicine groupsand vinblastine groups on the metaphases, in the experiment of prepare mouse4-cell embryo stage single blastomere chromosome specimen by using optimumparameters; and there were significantly differences between colchicine groupsand vinblastine groups on the metaphases (P＜0.05), in the experiment of preparemouse 8-cell embryo stage single blastomere chromosome specimen by usingoptimum parameters.The end, the preparation quality of mouse 4-and 8-cell embryo stage singleblastomere specimen were 2.5, 2.7, 2.4, 1.9, respectively.

低渗液选用0.45％氯化钠时，低渗效果优于选用0.5％柠檬酸钠和0.35％氯化钾的。6、在利用最佳参数制备小鼠4-细胞期胚胎单卵裂球染色体标本时，经统计分析，两种阻断剂的作用效果差异不显著；而在制备小鼠8-细胞期胚胎单卵裂球染色体时阻断剂选用长春花碱的效果优于秋水仙素的(P＜0.05)。7、秋水仙素组和长春花碱组小鼠4、8-细胞胚胎单卵裂球染色体标本制备质量分别为：2.5、2.7、2.4、1.9。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。