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Physicians certified in Transfusion Medicine are trained in blood product selection and management, immunohematology, apheresis, stem cell collection, cellular therapy, and coagulation.

在输血医学上获得资格的内科医生,要在血液产品选择和管理,免疫血液学,析离术,干细胞采集,细胞疗法,血液凝固这些方面被培训。

Results A total of 12 587 single ciliated cells could be isolated from nasal epithelium of each guinea pig.

结果 从每只豚鼠的鼻粘膜平均可分离出单离纤毛细胞(12 587±2 437)个。

Objective To study the effects of cisplatin on the inducible nitric oxide synthase expression in isolated cochlear auditory cells.

目的 探讨顺铂对耳蜗单离听觉细胞诱导型一氧化氮合酶表达的影响。

The early events in cotyledon cells cultured in vitro of Acacia mangium were studied under light and electron microscope.

用光学和电子显微镜观察马占相思子叶在离体培养中的细胞早期变化。

Objective To investigate the effects of selenium and fluoride on the cell cycle and apoptosis of human hepatocytes in vitro.

目的 研究硒、氟对离体人肝细胞周期和凋亡的影响。

Objective: To explore the characteristics of the synaptic transmission from contralateral ventrolateral funiculus to motoneurons in neonatal rat spinal cord slices.

目的:了解对侧腹外侧索至离体脊髓运动神经元突触传递的细胞电生理特性和介导递质。

ObjectiveTo study the effect and mechanism of menoprogen,on ovary granulose cells' apoptosis from female rats in vitro.

目的 探讨宁心红杞含药血清对离体大鼠卵巢颗粒细胞凋亡的影响及其机制。

This dissertation theoretically demonstrates the feasibility to achieve constant direct current-stimulation-circuitry under microampere , bring forward design scheme software and hardware system using in cellular electrical stimulation. In order to further quantitatively research on direct current-stimulation and ascertain the best intensity and density and stimulative current , the quantitative and qualitative analyse for gained diagnostic data are also been discussed in this paper.

本文正是从该理论基础出发,论证了实现微安级直流电刺激的离体组织细胞生长培养实验系统的可行性,提出了用于刺激实验的软硬件系统的开发放案,并对采集数据的定量和定性的分析作了初步的探讨,希望通过实验能够初步确定适合该组织细胞生长培养的最佳刺激电流和最佳刺激时间。

METHODS:Guinea-pigs were sensitized by ovalbumin and the effects of LMWH on the constriction of ileum smooth muscle induced by OA were observed in vitro;The effects of LMWH on the degranulation of pericranium mast cell induced by OA were observed throu gh the passive anaphylaxis ratmodel which was made by vaccinating antiserum at pericranium;Licking response was induced by 4-aminopyridine(4-AP) scat scruff in mice,then the effect of LMWH on licking response was studied the capillary p emeabilty increasing induced by glactal acetic acid and the effect of LMWH on ca pillary pemeability were studied.

用卵白蛋白(ovalbumin,OA)免疫豚鼠造成过敏模型,观察LMWH对OA所致的豚鼠离体回肠平滑肌收缩的影响;在大鼠颅骨骨膜接种抗血清使肥大细胞被动致敏,观察LMWH对OA引起肥大细胞脱颗粒的影响;以4-氨基吡啶诱发小鼠舔体反应,观察LMWH对舔体反应的影响;以冰醋酸使小鼠毛细血管通透性增加,观察LMWH对通透性的影响。

Mechanical strains also regulated the protein and mRNA expression of several differentiation markers, as well as the activation of extracellular signal-regulated kinases, p38 MAP kinase and protein kinase B in a frequency-dependent manner. Furthermore, the inhibition of p38 pathway could block the strain-frequency induced the phenotype modulation of VSMCs, neither ERKs nor Akt. Frequency of mechanical strain, not conditioned medium, regulated the phenotype of VSMCs in a frequency-dependent manner. Rho-GDI alpha was suppressed by the mechanical strain at 1Hz.

采用免疫细胞化学法检测VSMCs形态和排列的变化;RT-PCR和Western blotting检测表型标志分子α-肌动蛋白、肌球蛋白重链(SM1/2)、肌动蛋白相关蛋白SM22α和调宁蛋白(h1-calponin)的mRNA和蛋白水平的变化;抑制剂或RNA干扰阻断可能的信号调节分子的活性或表达,包括p38、细胞外信号调节激酶1/(2extracellular signal-regulated kinases, ERK1/2)、蛋白激酶B和Rho-鸟苷酸解离抑制因子(Rho-guanine nucleotide dissociation inhibitor, Rho-GDI alpha),研究了不同频率张应变对VSMCs表型转化的影响及其调节机制。

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