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During epileptogenesis produced by continuous epileptiform discharges or status epilepticus in different models of epilepsy, i levels were increased. Following the cessation of SE and electrographic seizure activity, i levels remained significantly elevated.

在不同的离体和在体癫痫模型中,均发现持续的癫痫样放电或癫痫持续状态可导致细胞内钙离子的浓度升高,在癫痫持续状态和癫痫样放电活动停止后,细胞内钙离子水平仍持续升高。

Intracellular recordings were made from neurons in the superfused isolated carpretina and the effects of calcium on synaptic transmission from cones and rods to horizontal cells were studied by changing the ionic composition of the external medium.

工作应用细胞内记录技术,在灌流的卿鱼离体视网膜的标本上,研究了低钙对外网状层中视锥、视杆信号向水平细胞传递的不同影响。

The decrease of granule Ca content occurred earlier than the increase of epinephrine concentration, suggesting that the calcium released from chromaffin granule may in part account for the induced cell secretion.

激动剂作用引起的离体肾上腺髓质细胞分泌时颗粒内钙含量的下降早于颗粒数目减少或肾上腺素升高,提示颗粒释放的Ca2+可能是引起细胞分泌的原因之一。

Methods Control the methanol feeding in growth phase of Hansenula polymorpha by online monitoring. In induction phase, the expression levels of HBsAg when the methanol feeding were controlled by DO-Stat/pH-Stat method and by offline gas chromatography, as well as those when only methanol was fed and glycerol and methanol were fed alternatively, were compared.

在细胞生长阶段采用DO/pH线测量的控制方法,使酵母细胞密度达到一个较高水平,诱导期比较DO-Stat/pH-Stat法和离线检测甲醇两种控制流加甲醇的方法,同时也比较用甘油和甲醇双碳源间断流加法与单纯流加甲醇法对目的蛋白表达的影响。

Histological observation showed that normal structure was observed in all the groups before being harvested or after being preserved for 30 minutes. However, the more severe structure damage was observed in HPCs or hepatic sinusoidal cells with the more prolonged rewarm ischemia.

形态学观察发现,四组供肝于离体前及低温保存30分钟时的组织结构均无明显异常改变;而在复温缺血期间,各组供肝肝实质细胞及肝窦内皮细胞则表现有不同程度的结构损伤,且在程度上也与复温缺血时间呈明显的平行关系。

Objective To investigate the expression of T lymphoma invasion and metastasis inducing factor 1(Tiam 1) in gastric cancer cells and analyse the correlation between Tiam 1 and the invasive and migratory potential of gastric cancer cells in vitro and in vivo.

目的 检测胃癌细胞中T淋巴瘤侵袭转移诱导因子1(Tiam 1)的表达,并分析其与胃癌细胞离体、在体浸袭转移能力的关系。

The calf intestinal epithelial cells in vitro were selected to evaluate the trophic actions of insulin, IGF-J and peptic and tryptic hydrolysates of bovine colostrum. The cell proliferation, glucose absorption and specific activity of cell membrane enzymes were investigated.

为研究乳源性生长因子对动物胃肠道生长发育的作用,本试验以离体犊牛小肠上皮细胞为试验对象,研究了胰岛素、胰岛素样生长因子Ⅰ和酶解牛初乳对细胞增殖、葡萄糖吸收和细胞膜酶活性的影响。

Up to now, problems of the cell differentiation mechanism and immunological rejection of xenogenic resource ESCs meeted during clinical application were still not resolved.

目前异体来源的ESCs在临床应用上还需要攻克细胞分化机制、免疫排斥等问题,而治疗性克隆获得的ESCs虽可以解决免疫排斥问题,但其技术复杂、离分化完全的功能细胞距离较远。

Further studies are underway to investigate the exact intracellular mechanisms underlying the effects of the two substances. In Chapter 4, we observed the effects of metabolites of mangrove fungus Xylaria sp. from the South China Sea Coast, Xyloketal A~D, on the activity of acetylcholinesterase in vitro, and the present data indicated that AChE activity was remarkably inhibited by Xyloketal A~D in a dose-dependent manner.

另一方面,在论文第3章离体豚鼠回肠收缩实验中,发现323-A和323-B可剂量依赖性地抑制组胺、乙酰胆碱以及氯化钾所引起的GPI收缩,pD2'依次为5.13、4.97、5.36和5.51、5.56、5.62,并且,通过机制分析发现,323-A和323-B可以显著降低在外界无钙离子存在时由细胞内钙储库释放钙所产生的收缩反应,而对由细胞外钙离子内流所引起的收缩无明显影响。

Objective Cultured RK13 and Tn-5B1 animal cell lines were induced with heat-killed Esherichia coli DH5α or Staphylococcus sarophyticus.

目的 利用诱导的离体动物细胞系兔肾细胞RK13和昆虫细胞Tn-5B1产生抗菌活性肽类物质,筛选具有抑菌作用的抗菌药物。

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