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To verify our speculation, we investigated the functions of the voltage-dependent potassium channel and large conductance calcium-dependent potassium channel of VSMCs isolated from hindquarter arteries and cerebral arteries of rats after short- and mid-term simulated weightlessness and compared with that of control rats, using both pharmacological methods and whole cell patch clamp techniques.

为证明我们的推想,我们采用动脉血管环药理学实验方法及全细胞膜片钳技术,来观察模拟失重大鼠脑部及后身动脉VSMCs的两种钾离子通道电压依赖性钾离于通道LK*与大电导钙激活钾离于通道[BK*)功能的改变并注意此变化的时程特征。

This in vitro study on toads provides the first electrophysiological evidence that electrical stimulation of the decussatio Veli in the midbrain tegmentum predominantly produced inhibition on neurons extracellularly recorded from the nucleus isthmi .

本文用离体脑片的方法直接研究了脑脚核团-峡核投射的突触连接性质,第一次证明,电刺激中华大蟾蜍中脑Veli交叉,在峡核内胞外记录,大多数细胞呈抑制反应,少数细胞呈兴奋反应。

Objective: To study the effects of progesterone on action potential of isolated rabbit sino atrial node cells.

目的 :通过观察孕酮对离体家兔窦房结细胞动作电位的影响,来探讨孕酮对窦房结细胞电活动的影响及其作用机制。

The hypercholesterolemic rats were selected to imitate early stage of human atherosclerosis The effects and mechanism of n-3 PUFA on endothelium were investigated with the following parameters: the morphological appearance and arrangement of endothelium stained by Ag, in vitro endothelium-dependentrelaxation of aortic ring, endothelial permeability test using Even′s Blue, blood lipid and body weight in one-week intervals, excitative and emissive spectrum of RBC membrane labeled by DPH (1, 6-Diphenyl-1, 3, 5-hexatriene), fluidity of RBC membrane, plasma and RBC membrane LPO

在整体和离体水平,选用大鼠高脂血症模型模拟动脉粥样硬化早期状况。用114只大鼠多指标着重研究n-3多不饱和脂肪酸在高脂血症状态下对内皮的保护作用,以探讨其预防动脉粥样硬化的机理。所采用指标包括:浸银法观察内皮细胞排列与形态;离体血管环观察内皮依赖性舒张;伊文氏蓝染料法观察内皮通透性。为探讨作用机理,进行了血脂和体重的动态监测、DPH(1,6-二苯基-1,3,5,乙三烯)标记红细胞膜的荧光光谱测定、红细胞膜流动性测定、血浆和红细胞膜脂质过氧化物测定。

Objective To investigate the protective effect of basic Fibroblast Growth Factor on guinea pig utricular hair cells destroyed by Neomycin in vitro.

目的 观察碱性成纤维细胞生长因子(basic Fibroblast Growth Factor,bFGF)对新霉素所致离体椭圆囊毛细胞损害的保护作用。

Results For callus induction of leaf explants of C. orchioides, dark treatment gave better results compared to light treatment; among the media tested, the suitable phytohormone combinations were 2.0 mg/L 2, 4-D or 6-BA 1.5 mg/L 2, 4-D 2.5 mg/L, and 300 mg/L CH 0.2% AC was good for plantlet regeneration from leaf explants. The callus from leaf explants mainly originated from midrib. The parenchyma cells near epicuticle of midrib firstly were initiated to division. Then the parenchyma cells of vascular bundle sheath and mesophyll cells on each side of vascular bundle were also divided to form callus.

结果对仙茅叶片的愈伤诱导,黑暗的效果好于光照;在所试验的培养基成分中,适宜的激素配比是2.0mg/L2,4-D或6-BA1.5mg/L 2,4-D2.5mg/L,并且附加300mg/L水解酪蛋白和0.2%活性炭,对于仙茅叶片的离体成苗较好;培养后,愈伤组织主要由叶片的中脉产生,位于中脉上表皮内侧的薄壁细胞首先启动分裂,随后维管束鞘薄壁细胞及其外侧的叶肉细胞也启动分裂,参与愈伤组织的形成。

To investigate the effect of lactate solution of different concentration on action potential of ventricular papillary muscle cell and contractile force.

应用细胞内微电极技术和微拉力描记法,研究不同浓度乳酸对家兔离体心室乳头肌收缩力(n=10)、心肌细胞动作电位(n=6)的影响。

L-arginine, an NO precursor, reduced the contractile amplitude and end-diastolic cell length in the cardiomyocyte; but reversibly increased LVDP,±dp/dtmax, and coronary flow in isolated perfused rat heart.

2NO的前体L-精氨酸(L-arginine, L-Arg)引起心肌细胞舒张期细胞长度缩短、收缩幅度降低。离体灌流心脏LVDP、冠脉流量、和±dp/dtmax增高,用K-H液复灌后可恢复正常。

Arginine, a NO precursor, reduced the contractile amplitude and end diastolic cell length in the cardiomyocyte, but reversibly increased LVDP,眃P/dtmax and coronary flow in isolated perfused rat heart.

-Arp预处理,再行Fe-HQ(5卜mol/L)灌流,与单纯的LAfg或Fe-HQ组相比,心肌细胞舒张期细胞长度、收缩幅度和速度减小;离体灌流心脏LVDP、tdP/im.x、心率和冠脉流量明显下降,冠脉流出液中LDH、CK增加。

Objective To investigate which stage is the most important and the relationship with kupffer cell,during isoflurane decrease anoxia-reoxygenation injury in isolated rat liver.

论文天下提供的免费论文,内容包括异氟醚离体鼠肝缺氧-复氧损伤枯否细胞缺氧复氧损伤细胞

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