离细胞的

Objective To further understand the intracellular calcium mobilization features of the isolated ampullate crista vestibular hair cells and ATP effects on intracellular free calcium concentration(［Ca2+］i) in VHCs of the guinea pig.

目的 探讨离体前庭毛细胞（vestibular hair cells，VHC）去极化时细胞内钙离子活动特征及内耳活性物质ATP对离体VHC内钙离子浓度的影响及其机制。

On the basis of this system andpreviously morphological, physiological and biochemical studies on cucumber in vitrogynogenesis. Materials from both typical gynogenesis using M99 as induction medium andnon gynogenesis using W5 as induction medium were collected. The activities anddistribution of peroxidase and its isoenzymes, content of soluble protein and distribution of allprotein during early stage of cucumber in vitro gynogenesis and non gynogenesis wereinvestigated by biochemistry and histochemistry technology.

本试验在前期有关离体黄瓜雌核发育形态学和生理生化研究的基础上，以诱导离体雌核发育的 M99 培养基上和非雌核发育的 W5 培养基上培养的黄瓜未受精子房为试材，采用生物化学技术和组织化学技术，深入研究了离体雌核发育早期外植体内的标志酶——过氧化物酶及其同工酶的活性及分布的变化、可溶性蛋白质的含量和总蛋白质分布的变化及核酸和多糖分布的变化，为揭示离体雌核发育早期的细胞分化机制提供科学依据。

Purpose: 1. Zonular fibers are a specific form of extracellular matrix composed mainly of fibrillin what believed to be secreted by non-pigmented ciliary epithelium. The variation of fibrillin-1 distribution in lens capsule after zonule rupture was investigated in the research.

目的：1。晶状体悬韧带是一种特殊的细胞外基质，主要由睫状体非色素上皮细胞分泌产生的fibrillin-1（微纤维蛋白-1）所组成，本研究的目的是观察悬韧带离断后，晶状体囊膜中fibrillin-1的变化，探讨悬韧带离断对晶状体囊膜组成的影响；2。

In the rice leaves infected by RDV Yunnan isolate, the distribution of cells full of virus particles formed a belt which was a center of infection and multiplication of virus, around the cells fall of virus particles were some cells with the different extent of lesion as follows: disappearance of membrane system of chloroplasts, there were virus particles in some chloroplasts, and some sine or assembled virions in the mitochodria or cytoplasma but not in nuclei ,and intact subcellular structure in the cell far away from the infected center; In the rice leaves cell infected by Zhejiang isolate, some cells were full of virions, but the distribution of cells didn't form the belt, other cytopathological characters were similar to the cells infected by Yunnan isolate.

RDV云南分离株浸染的水稻叶片中，充满病毒粒体的细胞呈条带状分布，呈现出明显的侵染和增殖中心，在充满病毒粒体的细胞周围，相邻细胞有不同程度的病变，叶绿体膜系统消失，部分叶绿体中含有病毒颖粒，线粒体残体及细胞质中有分散或聚集的病毒粒体，细胞核中未见病毒粒体，离侵染中心较远的细胞中亚细胞结构较完整；RDV浙江分离株侵染的水稻叶片中，部分细胞充满病毒粒体，无明显的条带状分布，其他细胞病理特征与RDV云南分离株侵染的水稻叶片相似。

FCM analysis showed that the apoptotic rate of MDA-MB-231 cells and intracellular Ca(superscript 2+) concentration increased gradually with the increase of deguelin concentration. RT-PCR and Western blotting analysis showed that the expression of Bcl-2 mRNA and protein was clown-regulated and that of Sax was op-regulated after deguelin treatment.

流式细胞术检测显示细胞凋亡率和细胞内钙离于浓度随鱼藤素浓度的增加而增多；RT-PCR和蛋白印迹检测结果发现鱼藤素能使Bcl-2 mRNA和蛋白表达减少，而Bax表达增加。

The ameloblasts are the key cells during odontogenesis, and they have been already cultured in vitro. The amelogenin is the extracellular matrix synthesized and secreted by the ameloblasts, and it plays a crucial role during amelogenesis.

成釉细胞是牙器官形成的关键细胞，国内外已成功离体培养出成釉细胞，成釉细胞合成和分泌的细胞外基质－釉原蛋白在釉质的形成中起着关键作用。

It is very important to biological treatment of angiogenesis diseases such as hypoxic/ ischemic cerebral diseases and cerebral ischemia/ reperfusion.PC 12 cells were treated with cobalt chloride (125uM) for 0、1、 2、4、8、12、16 hours.

离体培养的PC12细胞长满瓶底后，加入终浓度125uM氯化钴的培养液，孵育0、1、2、4、8、12、16h，检测细胞培养上清液中LDH的漏出；并收集缺氧0、1、4、8、16h细胞，戊二醛固定，切片，电镜观察细胞超微结构的改变。

Furthermore there are more apoptosis cells in inner root sheath than in outer root sheath. Conclusion When we culture human hair follicle in vitro, Williams E serum-free medium is a suitable choice.This model is a fine model also, which is used for screening drug that may accelerate or inhibit the hair growth.Cryo-section can simplify procedure of paraffin section in histology detection of culture human hair follicle in vitro.We can observe the ultrastructural change of human hair follicle in vitro by transmission electron microscope,which can assist to observe apoptosis cells. The eyepiece micrometer is a simple tool for measuring length of hair follicle in vitro.

在离体培养人头皮毛囊时，Williams E无血清培养基是合适的选择，该模型也是筛选促进或抑制毛发生长药物的良好模型；离体培养毛囊组织学检测用冰冻切片可以简化步骤；透射电镜可以用来观察离体培养的毛囊的形态和超微结构的变化，也可以用来协助观察细胞凋亡的状况。

Lymphocytes and monocytes separated from blood and head kidney of silver carp were cultured in the medium added with LPS stimulator.

将从血液和头肾中分离的淋巴细胞和单核细胞离体培养的过程中，添加LPS刺激培养细胞，收集培养液并利用亲和层析和电泳的方法，证实了经过LPS刺激的细胞培养液中有至少有3种细胞产物出现。

By removed ovary culture after positive and negative hybridization, produced interspecific hybridization, and have improved the hybrid seed through removed ovary culture and induced to shoot by culture medium MS, and produced hybrid F1, the plant of Brassica junce×B.pekinesis, the advantage characteristic of hybrid plant is similar to female nature;By cytology authenticating, chromosome number of F1 young flower bud cell (2n=28), it is the sum of monoploid chromosome number of female parent Brassica junce(2n=36) and male parent B.pekinesis(2n=20); The hybridization to Brassica junce×B.pekinesis, and embryonic bud come from removed ovary culture, and it found another form of plant embryo saving.

以芸薹属植物芥菜型油菜和白菜为研究对象，对其进行常规正反杂交后子房的离体培养，通过离体子房培养获得的杂种种子经MS培养基诱导成苗，获得了芥菜型油菜与白菜的杂种F1代植株，表现出杂种优势；通过细胞学的方法，鉴定了杂种F1幼蕾体的细胞染色体数为2n=28，为母本芥菜型油菜（2n=36）和父本白菜（2n=20）的单倍体染色体数之和；芥菜型油菜与白菜常规杂交后，经离体子房培养获得了&胚性芽&，确立了植物胚挽救的另一种形式。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。