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The relationship between the cardiotonic effects and the cardiac sarcolenmmal Na〓, K〓-ATPase activities by strophanthidin in isolated guinea-pig heart

本研究通过对正常和衰竭的离体豚鼠心脏和急性分离的心室肌细胞的研究,在器官水平分析了毒毛旋花子苷原的强心作用及其与Na〓,K〓-ATP酶活性间的关系,在细胞水平分析了毒毛旋花子苷原提高心肌收缩力的可能机制。

The isolated nori cells or protoplasts can be cultivated into thalli for the totipotency of plant.

由于植物细胞的全能性,解离的单细胞或原生质体经培养能够正常发育成叶状体。

Specifically, we will (1) perform electrophysiological study on the interaction of CTXs with cardiomyocytes stripped of specific GAGs,(2) study the binding of CTXs from spitting cobra venom with corneal tissue and examine the possible involvement of GAGs in the disruption of regular superstructure of collagen fibril and (3) examine the role of polyproline peptide sequence of P-type CTXs in the lytic action of CTXs. The results of this study will not only help delineate the mechanism of cobra CTXs, a long standing problem in the toxicity of cobra venom, but also suggest a new approach to develop CTXs into a potential drug for cancer.

本计划将进行:心脏细胞在去除醣胺基前后的电生理研究及比较了解心脏毒素与眼角膜的结合情形,并探讨醣胺基在维持眼角膜透明度所扮演的角色探讨P形心脏毒素中所含脯胺酸对细胞溶离能力的影响我们预期本研究将可对心脏毒素的作用机制有深入的了解,并寄望在未来能利用所了解的新作用机制,将心脏毒素发展成能控制癌症的新药方。

Effect of Astragalus radix on proliferation and nitric oxide production of head kidney macrophages in Cyprinus carpio:an in vitro study .

黄芪对鲤头肾中巨噬细胞的增殖和一氧化氮产量的影响:离体研究。

Objective: To study the effect of paeonol on the proliferation rate and tissue plasminogenactivator in mesothelial cells.

目的:探讨丹皮酚影响下离体间皮细胞的增殖及分泌组织型纤溶酶原激活剂的情况。

We have, therefore, investigated the direct effects of adiponectin on primary cultures of osteoblastic and osteoclastic cells in vitro and determined its integrated effects in vivo by characterizing the bone phenotype of adiponectin-deficient mice.

为此,我们研究了脂联素对离体培养的原代成骨细胞和破骨细胞的直接影响,并通过对脂联素缺陷小鼠的骨基因表型的研究明确其在体内的综合作用。

Most techniques in vivo monitoring the distribution, prolification and migration of transplanted cells. To objectively evaluate curative effect after cells transplantation, most techniques for the study of stem cell transplantation in animal models require histological analysis to determine the fate and migration of cells.

监测活体内移植入细胞的分布、增殖、迁移的方法虽然有多种,但传统方法都需要在离体状态下进行组织学切片分析和鉴定,无法准确地观察植入细胞的生长分化情况,从而难以对干细胞移植术的疗效作出客观评价。

Therefore, fluorescein isothiocyanate conjugated lectins, namely concanavalin agglutinin, wheat germ agglutinin and soybean agglutinin, were used to localize lectin receptors on plasma membrane of male and female gametes and other relevant sexual cells, as well as embryos of various developmental stages of Nicotiana tabacum and Torenia fournieri by cooled charge coupled device and Confocal Laser Scanning Microscope.

从而证实:在本实验中用的几种酶组合对凝集素受体的影响并不大,即由酶解及解剖得到的雌性细胞的荧光在分布形式及强度上没有明显的差异;对比原位酶解与离体酶解得到的雌性细胞的荧光,证实机械操作对荧光的分布形式及强度也没有明显的影响。

Isolated plant cells have totipotency , it can develop into a whole plant. under some isolated conditions, every cell can develop into a whole plant as the same as matrix plant

离体的植物细胞具有发育上的全能性,能够发育成为完整的植物体,在一定培养条件下每个细胞都可发育成一个与母体一样的植株。

Objective To further understand the biological features of the human vestibular organ.

目的 进一步了解离体人前庭毛细胞的胞内钙离子(Ca2+)活动。

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