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Anatomical experiment on slice, eduction and transparency of leaves of Cedrus deodara were conducted to study the number of resin canal, cell structure and the whole eduction observation.

通过常规切片和整体透明离析处理的方法对雪松针叶树脂道进行全面的解剖学研究,描述了雪松针叶树脂道的数目和位置、树脂道细胞的结构,对树脂道进行整体离析观察。

In order to evaluate the effects of calcium ion on calpalns mRNA expression and proteolysis activity,and muscle protein degradation,L6 myoblast and beef fascicle were used in vivo and in vitro samples respectively in this study.

本实验分别以牛肉肌束和分化为肌细胞的L6成肌细胞为研究对象,研究钙离子对离体肌束中钙激活蛋白酶活性、蛋白分解以及活体肌肉中钙激活蛋白酶mRNA水平表达的影响。

METHODS AND RESULTS: Adherent platelets express substantial amounts of SDF-1 and recruit CD34+ cells in vitro and in vivo. A monoclonal antibody to SDF-1 or to its counterreceptor, CXCR4, inhibits stem cell adhesion on adherent platelets under high arterial shear in vitro and after carotid ligation in mice, as determined by intravital fluorescence microscopy.

方法和结果:在体和离体的粘附血小板表达大量SDF-1、募集CD34+细胞,在体荧光镜下可见,在小鼠颈动脉结扎后、离体条件下的动脉内高剪切应力时,SDF-1或其相应受体的单克隆抗体-CXCR4抑制干细胞粘附于血小板上。

Standard patch clamp methods were used to measure whole cell capacitance,zero current potential,reversal potential and potassium ion currents of Deiters′ cells in normal external solution.

目的:分离豚鼠内耳活性良好的单离Deiters细胞,观察其形态;探讨该细胞的基本电生理特性。

Different doses (25,50 and 100 mg/L)of gentamicin were add to the medium of SGC,and cultured for 14 days.After that,the specimen was fixed with 4%paraformaldehyde and stained with toluidine blue.

为观察硫酸庆大霉素对培养的小鼠耳蜗螺旋神经节细胞的细胞生存数和细胞突起长度的影响,在单离培养的出生1-5 d的NIH小鼠SGC培养液中,加入不同浓度的硫酸庆大霉素(25,50,100 mg/L)。

In the present study, the effects of chloride channels blockers,(Niflumic acid,NFA;IAA-94;DIDS) on EJP were explored in guinea pigs, using intracellular recording techniques on acute isolated spiral modiolar artery.

本实验运用细胞内微电极记录技术,在豚鼠耳蜗螺旋动脉(spiral modiolar artery, SMA)离体标本上,研究氯通道阻断剂(Niflumic acid, NFA; IAA-94; DIDS )对去甲肾上腺素引起SMA平滑肌细胞的反应和平滑肌细胞EJP的影响。

Methods Neomycin was used to destroy guinea pig utricular hair cells in vitro.The culture in experimental groups was supplemented with bFGF.The culture in control group was not supplemented with bFGF.All utricles were observed by scanning electronic microscope,and the number of survival utricular hair cells was accounted.

采用离体的豚鼠椭圆囊培养法,对照组为普通前庭培养液培养,实验组按培养液中含和不含bFGF分组,用新霉素造成各实验组培养的椭圆囊毛细胞损伤,各组椭圆囊均行扫描电镜检查并行毛细胞计数。

ONOO〓 had direct vasoactive effect for relaxation of precontracted isolated pulmonary artery. Relaxing action of ONOO〓 was weak and was negtively regulated by endothelial cells, further supporting the notion that ONOO〓 may be involved in pulmonary hypertension in the early stage of ES.

用离体血管环检测张力方法,发现ONOO〓具有一定的舒张肺动脉作用,但舒张效应较弱,并受到血管内皮细胞的抑制性调节;抑制K〓通道和PARS活性可增强ONOO〓对离体兔肺动脉的舒张作用。

The effect of gentamicin on survival and neuritogenesis of cultured spiral ganglion cell from NIH mouse 1-5 days after birth was observed.Different doses (25,50 and 100 mg/L)of gentamicin were add to the medium of SGC,and cultured for 14 days.

为观察硫酸庆大霉素对培养的小鼠耳蜗螺旋神经节细胞的细胞生存数和细胞突起长度的影响,在单离培养的出生1~5 d的NIH小鼠SGC培养液中,加入不同浓度的硫酸庆大霉素(25,50,100 mg/L)。

The Plant tissue raise is the plant sterile culture technology, has the totipotency theory according to the vegetable cell, the use plant body exsomatize organ (for example root, stem, leaf, stem point, flower, fruit and so on), the organization (for example cambium, epidermis, cerebral cortex, marrow department cell, endosperm and so on) or the cell (for example big spore, small spore, somatic cell and so on) as well as the protoplast, in aseptic and suitable artificial culture medium and illumination, temperature and so on under artificial condition, can induce injuries the organization, not to decide the bud, the adventitious root, finally forms the complete adult plant the discipline.

植物组织培养即植物无菌培养技术,是根据植物细胞具有全能性的理论,利用植物体离体的器官(如根、茎、叶、茎尖、花、果实等)、组织(如形成层、表皮、皮层、髓部细胞、胚乳等)或细胞(如大孢子、小孢子、体细胞等)以及原生质体,在无菌和适宜的人工培养基及光照、温度等人工条件下,能诱导出愈伤组织、不定芽、不定根,最后形成完整的植株的学科。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。