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As antioxidants, the vitamin E or quercetin (10μg/mL) significantly attenuated the toxic effects of PCBs on the cultured hepatocytes. The above results indicated that vitamin E or quercetin might antagonize the toxic effect of PCBs via antioxidizing effects on in vitro cultured chicken embryo hepatocytes.

结果表明离体培养的鸡胚肝细胞可作为肝损伤的细胞模型,PCB对培养的肝细胞的损伤及抗氧化剂维生素E和槲皮素对它的缓解作用提示, PCB对肝细胞的损伤可能是通过诱导细胞膜发生脂质过氧化而导致细胞的死亡。

That GER cells which are likely hair cell progenitors could be cultured in vitro and generate stereociliary bundle-like structure when forced to express Hath1 suggests that the misexpression of Hath1 probably can induce the predifferentiation of pure hair cell progenitors into hair cells in vitro.

GER细胞作为毛细胞前体细胞可以实现体外原代培养,在Hath1基因重表达情况下,部分细胞的表面可见不成熟的纤毛样结构,提示Hath1基因也许可以诱导离体培养下的纯的毛细胞前体细胞向毛细胞的初步分化。

The neurosecretory somata were located onthe proximal surface of the medulla terminalia of the eyestalk.Three types ofneurosecretory cells were distinguished on the basis of size, morphology,distribution,form of outgrowth and ultrastructural feature.

河蟹眼柄神经分泌细胞主要分布于眼柄视神经节端髓基部外侧,依据细胞的大小、形态、超微结构特征及离体生长形态区分出三种类型神经分泌细胞。

The result is consistent with previous studies. Whereas, tapetal cells of sterile anthers were disintegrated since tetrad cells were disjoined, indicating that disintegration of tapetal cells is earlier occurred in sterile anther than that in fertile anther. DNA ladder revealed that the cleavage of nuclear DNA into oligonucleosome-sized fragments to

结果表明,在花粉母细胞减数分裂后的四分体解离时期,不育花药的绒粘层细胞较可育花药提前开始裂解;在不育系S-Mo17rf3rf3花药和花粉S-(rf3)中均明显出现PCD过程的DNA片段化以及线粒体细胞色素C外渗的现象,证明了玉米CMS-S的花粉败育与花药绒粘层细胞的提前凋亡和小孢子细胞的细胞程序性死亡有关。

bFGF protected guinea pig utricular hair cells from Neomycin injury in vitro.

新霉素对离体的椭圆囊毛细胞有较强的破坏作用;bFGF对新霉素所致离体椭圆囊毛细胞的损伤有保护作用。

Results A significantly higher number of survival utricular hair cells was seen in experimental group(P<0.05),as compared with control group.Conclusion bFGF protected guinea pig utricular hair cells from Neomycin injury in vitro.

结果 含bFGF的实验组毛细胞的存活数目显著高于不含bFGF的实验组P结论新霉素对离体的椭圆囊毛细胞有较强的破坏作用;bFGF对新霉素所致离体椭圆囊毛细胞的损伤有保护作用。

Objevtive: To investigate the effect of effective parts of Zingiber officinal on the adhesion of ECV-304 cells with monocytes cultivated in vitro and on the expression of monocyte chemotactic protein-1 (MCP-1) and adhesion molecules.

中文摘要:目的:研究鲜姜有效部位对离体培养的血管内皮细胞与单核细胞的黏附力及对单核细胞趋化蛋白和黏附分子表达的影响,探讨其保护血管内皮细胞的作用机制。

In this experimental conditions, the VEC had not damnified obviously by perfusion with IECS in 4 hours. The pulmonary vascular resistance could decreased by POE1 and so vascular endolhelial cells would be protected. Mannitol not only increase osmotic pressure but also cleanse OFR. The addition of mannitol, POE1, glucose and osmotic pressure to IEC solution contributes to reduce the quantities of OFR.

2在本实验条件下所采用的IECS进行犬肺灌洗、离体保存4h之内肺血管内皮细胞无明显损伤,PGE;具有保护肺血管内皮细胞的作用;甘露醇具有增加渗透压和清除自由基的作用;灌洗前肺动脉内注射PGE;及增加 ECS渗透压和代谢底物、提高PH值使之略微偏碱均有助于抑制供体肺离体、低温、冷缺血保存期间OFR的产生。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型,取健康大鼠每日灌服不同剂量的鲜姜有效部位(200,400,800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1),取含药血清作为受试药物,用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力;逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平;酶联免疫吸附法测定MCP-1蛋白表达水平。

It has been reported that the mechanism of anti-parasite effect of ART may be associated with free radical which was yielded by iron ion within malarian.

作用P388细胞24 'J'时以提高细胞内铁离于浓度,再用ART作用48小时,发现其对P388细胞的生长抑制率高于单用ART。

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