离心的

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只，每组再分为高代谢脓毒症组和对照组两部分，建模方法同第一部分；2）三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸；3）灌注前抽取动脉血作为背景值，灌注达150，160，170和180min分别抽取动脉血2ml用于质谱分析；4）实验开始后前30min中每5min收集呼出气一次，随后每30min收集呼出气一次，用于气相质谱分析；5）血样经过低温离心取上清液，过阳离子及阴离子交换树脂，再经衍生化处理；6）处理过的血样进入气相色谱－质谱仪，测量其13C标记的亮氨酸、葡萄糖和乳酸丰度；7）实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度（E13CO2）；8）利用文献提供的公式算出CO2总生成率（V13CO2）以及机体各物质的通量、氧化速率以及物质代谢百分比等；9）数据分析处理采用t检验。

Sample collection: Two hundred and fifteen sera samples of normal population were collected from the peripheral blood of healthy blood donors of Shenyang, and the sera were separated by centrifugation after blood clotting; The patients sera were collected from the outpatients and inpatients of No. 1 hospital of China Medical University and the Second hospital of Jilin University. Syphilis was confirmed by clinical and serum test of TPHA and RPR; That of pemphigus vulgarism was confirmed not only by clinical, histopathological examination, but also by direct and indirect immunofluorescence test. DIF test on lesion or non-lesion skin showed immunoglobin and/or C3 deposits in the intercellular space of epidermis, and IIF test showed positive anti- intercellular antibody in patient serum using mouse's or rat's tongue or esophagus as substrate.

标本的收集：正常人的血清：采集沈阳市正常献血员外周静脉血共215份，待血凝后离心分离血清；患者的血清：来自中国医科大学一院及吉林大学二院门诊及住院部的病人，梅毒患者除了病史及临床表现外，全部经血清学TPHA及RPR确认；寻常性天疱疮患者除了临床及组织病理诊断外，还进行了直间接免疫荧光的检测，直接免诊断疫荧光：在皮损区及非皮损区的表皮细胞间显示IgG和/或C3补体沉积；间接免疫荧光用鼠或兔的舌或食管作为底物，在病人的血清中显示有肯定的细胞间抗体。

Significant processing steps can include: a phase changes involving either the desired molecule or the solvent, inert carrier or vehicle, e.g., dissolution, crystallization, evaporation, sublimation, distillation or absorption; b a phase separation such as filtration or centrifugation; c any chemical change involving the desired molecule, e.g., removal or addition of water of hydration, acetylization, formation of the salt; d an adjustment of the solution containing the molecule such as adjustment of pH or pO2; e a precision measurement of contained or added BPC components, in-process solutions, recycled materials is performed, i.e., weighing, volumetric measuring, optical rotation, spectrophotometric determinations, etc.; and f changes occur in surface area, particle size, or lot uniformity, e.g., milling, agglomeration, blending.

意义较大的步骤可能会包括：a相变化，包括指定分子或溶剂，惰性载体或溶媒，如溶出作用，结晶，蒸发，升华，蒸馏或吸附；b相分离，如过滤或离心；c涉及指定分子的任一化学变化，如结晶水的除去或添加，乙酰化作用，成盐；d包含分子的溶解的调节，如调节pH或pO2；e 含有或添加原料药组分，过程内溶液，回收物料的精密测量，如，称重，体积测量，旋光度，分光光度测定，等和f 表面，粒度，或批一致性的变化，如磨碎，凝块，混合。

Autologous MSC can be obtained easily,their source in human are plenty , own active proliferationand potential to difference into chondroblast.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Autologous MSC can be obtained easily, their source in human are plenty, own active proliferation and potential to difference into chondroblast. Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful, the standard technology is culture the cell-ball in centrifuge tube, the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Autologous MSC can be obtained easily,their source in human are plenty , own active proliferationand potential to difference into chondroblast. Now MSC become the chief seed-cell in cartilage tissue engineer , but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Using culture medium selection, diluted separation, plate lineration, centrifugal separation, capillary separation, droplet separation, pH separation, temperature separation, inhibitor separation and so on, ten species were obtained. There were Cyanophyta Nostoc punctiforme, Phormidium tenue, an undetermined alga; Chrysophyta Chromulina ovalis; Chlorophyta Cholorella ellipsoidea, Scenedesmus bijuga, S. quadricauda, Elakatothrix sp., Selenastrum minutum and Bacillariophyta Navicula sp.

采用培养基筛选法、稀释分离法、平板划线分离法、离心分离法、毛细管分离法、小滴分离法、pH值分离法、温度分离法、抑制剂分离法等9种不同方法，最终分离纯化出4门9属10种藻类，其中包括蓝藻门念珠藻属的点形念珠藻、席藻属的小席藻和一未定种；金藻门单鞭金藻属的卵形单鞭金藻；绿藻门小球藻属的椭圆小球藻、栅藻属的双对栅藻和四尾栅藻、纺锤藻属的一种、月牙藻属的小形月牙藻和硅藻门的舟形藻属的一种。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

After analysizing the results of SSRFEM and centrifugal model test, someconclusions are drawn that, the depth of slip surface implies that the Bishopssimplied method cannot indicate the true failure mechanism for the slopes reinforcedwith piles. The rate of safety factors decreases with the pile spacing decreasing, andthe salty factor of the slopes is the largest when the reinforced piles are installed in themiddle of the slopes, regardless of pile head confining conditions, and the deflection,maximum bending moment and shear force in the reinforced piles in the criticalcollapse are the biggest meantime. The fixed and hinged head condition of the pilesresults in smaller bending moment in the piles than that in unrotated and free headcondition, therefore the restrained pile head condition is recommended in the design.

3不同特性与工况下抗滑桩与土体相互作用的数值分析与离心模型试验研究表明：采用简化Bishop法计算得到的滑动面不能反映抗滑桩加固边坡的破坏机理，抗滑桩设置在边坡中部和减小桩间距有利于提高边坡稳定安全系数，而此时抗滑桩在边坡临界破坏时的挠度、最大弯矩和最大剪力相对较大；桩头固定、铰接与桩头自由、平移相比，所加固边坡的整体水平位移及边坡临界破坏时抗滑桩的最大弯矩相对较小，而稳定安全系数则较大，因此在进行抗滑桩设计时应尽量选择桩头固定与铰接的型式，或对桩头进行锚固以改善抗滑桩的受力特性，提高边坡的稳定安全性状。

Well, now, the best sutra laying in front of us, is to hold the three essentials of learning from Buddha, generate the mind of renunciation, know the sufferings of the temporal world and the life. We caused many evil karmas in the previous lives, so we shall return our merits and virtues to everybody, which can resonate among everyone and solve those obstacles of all our past lives that cannot be solved simply relying on our own strengths.

那么现在，摆在我们面前最好的一本经，就要是抓住学佛的三个根本，生起出离心，知道世间的苦，人生苦短，我们累生累劫造了很多障碍，我们要用我们的功德，回向给大家，使大家产生一种共同的心声，才能解决我们累生累劫的那些障碍，光靠我们自己的力量是不行的。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。