离心的

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

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长隆水上乐园还拥有国际旅游行业协会的荣誉推荐项目疯狂树屋池，它是世界上最大主题式的水上游玩区，有着各种不同的水滑道和玩水活动提供不同年龄层的游客无穷的欢乐；以及另一个国际旅游行业协会荣誉推荐项目急驰竞赛，加上离心滑道和高速滑道等水上游乐设备，都非常适合喜欢跳战刺激的年轻人。

Extraction of fish oil is done in the temperature of 65℃,70℃, 75℃,80℃,85℃,90℃and95℃ in turn in the condition of pH8.0,55mins in hydrolyzing,20mins in salting out with 4.5 percent of potassium nitrate and 30mins in centrifugalization with 3000r/min.

在传统淡碱水解工艺的基础上，将氢氧化钾和硝酸钾分别替换原工艺中的氢氧化钠和氯化钠，在pH8.0、水解时间55min、用4.5%的硝酸钾盐析30min、用3000转的离心机离心30min的条件下，分别在65℃、70℃、75℃、80℃、85℃、90℃、95℃时提取粗鱼油。

Methods Density gradient centrifugalization combined with adherence method were used to segregate and cultivate rat MSCs. MSCs cultivated to the 3 rd passage were characterized using flow cytometry technique. TGF-β1 with 1, 2 and 5 μg/L were pretreated on MSCs for 8 h, then serum and oxygen deprivation were used to cause analogous ischemia of MSCs in vitro. AnnexinV/PI flow cytometry technique was used to evaluate apoptosis and survival of MSCs.

采用密度梯度离心结合贴壁法分离、培养大鼠MSCs，对培养第3代的MSCs进行鉴定；用1、2、5μg/L的TGF-β1分别预处理细胞8 h，然后用血清剥夺和低氧处理建立MSCs体外模拟缺血性损伤模型，通过流式细胞术评价MSCs的凋亡及存活情况；用Western blot方法检测2μg/L的TGF-β1对Akt和p-Akt表达的影响。

Based on traditional technology of light alkaline bydrolysis,sodium hydroxide and sodium chloride in original technology will be replaced by potassium hydroxide and potassium nitrate differently.Extraction of fish oil is done in the temperature of 65℃,70℃, 75℃,80℃,85℃,90℃and95℃ in turn in the condition of pH8.0,55mins in hydrolyzing,20mins in salting out with 4.5 percent of potassium nitrate and 30mins in centrifugalization with 3000r/min.

在传统淡碱水解工艺的基础上，将氢氧化钾和硝酸钾分别替换原工艺中的氢氧化钠和氯化钠，在pH8.0、水解时间55min、用4.5%的硝酸钾盐析30min、用3000转的离心机离心30min的条件下，分别在65℃、70℃、75℃、80℃、85℃、90℃、95℃时提取粗鱼油。

In detail the process of the invention relates to a method for solubilising an oil-soluble pigment into an oil or fat by extraction of a solid preparation containing the oil-soluble pigment comprising the steps of a mixing the solid preparation containing the pigment with water, an extraction medium containing an edible oil or fat, and a nonionic surfactant having a hydrocarbyl group, an acyl group or a substituted hydrocarbyl or acyl group containing at least 6 carbon atoms; b optionally centrifugalize the mixture obtained and separate the oil phase.

具体而言，本发明方法涉及一种通过萃取含有油溶性颜料的固体制剂而使油溶性颜料在油或脂肪中增溶的方法，其包括步骤a将含有颜料的固体制剂与水、含有食用油或脂肪的萃取介质以及具有含至少6个碳原子的烃基、酰基或取代烃基或取代酰基的非离子表面活性剂混合，和b任选将所得混合物进行离心并且分离油相。

The preparation process includes the following steps: mixing 10 mM concentration water solution of chloropalladic acid and 12.5-25 mM concentration water solution of ammonium hexadecyl trimethyl bride in the volume ratio of 1 to 20, heating to 65-95 deg.c, adding 100 mM concentration water solution of ascorbic acid in the volume ratio to the water solution of chloropalladic acid of 0.16 to 1 to react for 30 min, centrifugal separation to collect precipitate, water washing, and drying to obtain cuboidal nanometer Pd material.

该钯纳米长方体材料的制备方法的步骤和条件法如下：把体积比为1∶20的10mM氯钯酸水溶液和12.5-25mM十六烷基三甲基溴化铵水溶液混合，加热至65-95℃，然后加入与10mM氯钯酸水溶液的体积比为0.16∶1的100mM抗坏血酸水溶液，反应30分钟，将溶液离心分离，收集到的沉淀用水洗涤，干燥，得到钯纳米长方体。

The colour of most detected bands became light in the five media with the order of mannosan, mannose, xylan, glucose and pectin.(4)The pectinase in the supernatant was purified by centrifuge , acetone precipitation and sephadex G-75 cloumngel filtration. The specific activity was increased 9.8-fold with an activity recovery of 32.98%.The molecular weight of pectinase was 42.2KD estimated by SDS-PAGE.

确立了以葡萄糖培养基6h的发酵液为提取果胶酶的最初取样点，发酵液经离心，丙酮沉淀，Sephadex G-75葡聚糖凝胶柱层析后获得了凝胶电泳均一的样品，比活力较原酶液提高了9.8倍，回收率达到了32.98%，用SDS-PAGE凝胶电泳测的纯化后的果胶酶分子量为42.2KD。

The resultant pellet was layered on a gradient composed of 1.2, 1.8 and 2 M sucrose layers in 50 mM Tris, 1% SDS and centrifuged at 106,000 g for 120 rain at 4 ° C. The material recovered at the 1.8-2 M interface was diluted in 0.1 M TRIS with 2% SDS and pelleted (106,000 g, 60 min).

由此产生的颗粒是层次上的梯度组成1.2 ， 1.8和2男蔗糖层50毫米的Tris ， 1 ％ SDS和离心在十点六万克120雨在4° C的材料回收在1.8 M界面稀释后在0.1米的招聘2％ SDS和包衣（十点六〇〇万克， 60分钟）。

We take spectrographic analysis for the chemical composition of tapping and UT for the bonding zone of rolls.

在炉前铁水成分分析中，采用国际先进的光学和化学分析相结合的方法，离心轧辊的结合层质量按国家标准采用超声波检测，具有完备的生产保证体系和先进的产品质量检测手段。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。