离心的

Results Multidrug resistance could be detected in the tumor tissue after the injection of retrovirus around and into it.After filtration and centrifugalization,the concentrated supernatant was 2 times efficient than the original supernatant and P-GP expression was detected in 60% of the tumor cells.mdr1 mRNA expression in the tumor tissue was significantly increased after gene transfer as detected by RT-PCR.

结果 携带mdr1基因的逆转录病毒上清液注射后肿瘤模型产生多药耐药性，经过滤和离心浓缩的病毒上清液60%细胞表达P-GP，是病毒原液基因转移率的2倍；RT-PCR表明基因转移后，肿瘤组织内mdr1 mRNA的表达量明显增加。

This investigation include two parts: lThe primary culture, chondroblast induction and evaluation of MSC. 2The chondroblast cultured with compound porous materials PDLLA and PDLLA/chitosan.

第一部分人骨髓基质干细胞的原代分离、体外培养、成软骨诱导及鉴定目的：了解骨髓基质干细胞原代培养的特性及增殖能力，探讨轻度离心和化学限定培养基法诱导骨髓基质干细胞向成软骨方向分化的作用；为软骨组织工程提供足量、合适的种子细胞。

Fruit a ± completely syncarpous 4-loculed drupaceous berry [or grading to subapocarpous with 4 basally connate drupaceous berries]; exocarp ± fleshy or dry; mesocarp, if evident, spongy-crustaceous or ± woody; endocarp cartilaginous.

果为多少完全合心皮的4室的具核果状浆果 [或逐渐变为近离心皮的具4室基部合生的具核果状浆果]；外果皮多少肉质或干燥；中果皮，如果明显，为海绵状壳质或者多少木质；内果皮软骨质。

Fig3-5 Profound Class II division1 protrusion of the maxillary incisors leads to condylar pressure against the articular eminences , as the mandible unsuccessfully seeks incisal contact in excusive movements.

图3-5很深的二级一类上颚门齿的前突，当下颚离心运动时无法成功的寻找门齿接触时，会导致髁头在关节隆凸的压迫。

When different materials mixed,the mixture will have a mixed melting point of mixture ,and the mixed melting point of mixture is different from the intersoluble solutions.we separate the intersoluble solutions by different melting piont of intersoluble solutions when separate temperature is flexible temperatureThese intersoluble solutions different in melting point, the method would make the high melting point material became solid while the low melting point material still be liquid by adopting cryogenic treatment with stirring so as to meet the requirement of purifying these two solutions.

这种分离方法的特点是利用两种互溶性物质具有不同的熔点，通过低温处理，并同时加以搅拌，震荡，使高熔点物质和低熔点物质形成固液混合体，再对固液混合体进行离心分离得到高熔点的固体和低熔点的液体，从而达到浓缩、提纯两种物质目的。

The result of frozen MⅡoocytes using three methods indcated that the self-restraintspear method was better than OPS, and the effection of frozen altogether with self-restraintspear method and centrifugal was the best; the frozen effection was not ideal using fluidspear for 8- cell ovocyte.

用三种冷冻方法(OPS法；自制移液枪头法；移液枪头法+离心法)对MⅡ卵母细胞冷冻的结果表明，自制移液枪头法优于OPS法，其中以移液枪头法+离心组的冷冻效果最好；用移液枪头法对体外发育到8-细胞期卵母细胞的冷冻效果不理想。MⅡ期卵母细胞用OPS法冷冻后卵母细胞IVF和电激活的卵裂率都显著高于移液枪头法(P＜0.01)，电激活后2-细胞卵裂率比IVF高。

The cultured cells appeared morphologically as spindle-shape and the average number of colonies which form in primary culture was 43±11. MSCs were positive for CD44 and CD29 but negative for CD34, CD45 and HLA-DR with a phenotypical identity of 99%.

上述结果表明，通过密度梯度离心方法分离培养的细胞不是骨髓造血干/祖细胞，而是一群形态和表型均一的间充质干细胞，具有独特的增殖和表型特征及较强的分化潜能。

Collect Agrobacterium cells by centrifugation at 4,000g for 10 min at room temperature, and gently resuspend cells in 1 volume of freshly made 5% sucrose solution with a stirring bar.

在 4，000g 的离心分离的 8|收集 Agrobacterium 细胞 10 分钟在室温，而且逐渐地以一根活泼的酒吧新做的 5%蔗糖解决在 1 册中再中止细胞。

1MM GTP was mixed with tubulin (2mg/ml) and varying concentrations of sodium selenite. The mixture was incubated at 37℃ for 1hr. MTs were separated from unassembled tubulin by centrifugation. Both supernatants and pellets were subjected to SDS-PAGE.

其中一组实验是在相同的微管蛋白体系中加入不同浓度的亚硒酸钠，然后加入GTP，37℃聚合，离心，沉淀为聚合的微管，而上清液为未聚合的微管蛋白。

The unflatness of amplified spontaneous emission noise and its impacts imposed on the performance of the off-center narrow filter polarization-nulling method when monitoring the in-band optical signal-to-noise ratio of dense wavelength division multiplexing system are analyzed, and a modified method by introducing an unflat factor in the calculation of OSNR is proposed.

分析了累积自发辐射噪声的非平坦特性，在已有窄带离心偏振置零法测量波分复用系统中带内光信噪比的基础上，分析该方法在噪声非平坦条件下存在的问题，提出了在光信噪比的计算中引入修正因子。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。