离心的

What sound accompanied the union of their tangent, the disunion of their centrifugal and centripetal hands?

385伴随着他们那相接触的手的结合，他们那离心的和向心的手的分离，传来了什么响声？

P28 紫色 Formation of a peptide bond 肽键形成–4侧链具有的反应–形成二硫键-其它反应 Tyr,His,Arg Review Isoelectric Point Titration滴定 of an amino acid 滴定 Gly幻灯片 18 His Lys Glu Reaction of amino acids Section 4 Protein isolation and purification 蛋白质分离及提纯 Working With Proteins Experimental techniques for protein analysis and characterization Purification steps A cell contains many types of proteins In the lab we want to isolate a single protein for experiments Purification steps We first grow cells or isolate tissues that contain the protein of interest We break open the cells to produce a crude extract Use centrifugation离心 to separate soluble from insoluble material We fractionate 分离 the protein mixture based on properties of such as size, charge affinity or solubility.

丹磺酰氯与氨基酸反应生成荧光性质强和稳定的磺胺衍生物，用于多肽链NH 用于多肽链 3末端氨基酸的标记烃基化反应(1) 2，4-二硝基氟苯2，4-dinitrofluorobenzene，二硝基氟苯(二硝基氟苯 DNFB也叫做试剂。DNFB在弱碱性溶液也叫做Sanger试剂试剂中与氨基酸发生取代反应，生成黄色化合物二硝基二硝基苯基氨基酸(dinitro phenyl amino acid， DNP氨基酸氨基酸)苯基氨基酸氨基酸(2)苯异硫氰酸酯(phenylisothiocyanate， PITC)在弱碱性条件下，与氨基酸反应在弱碱性条件下，生成苯乙内酰硫脲 PTH衍生物，(phenylthiohydantoin， PTH)衍生物，即PTH-氨基酸，此反应又称之Edman反应，该反应是蛋白质或多肽氨基酸序列测定常用的反应。

The reclamation rate of the former is 21% and the later is 36%.

但 PEG的沉淀效率要低于超速离心的效率，前者病毒的收率为21％，后者病毒的收率为36％。

In order to design the angle of self-destroying bevel in fuze's centrifugal ball device properly,widen the ballistic environment range is applied to the centrifugal ball device and improve the reliability of self-destruction action for centrifugal ball device,the self-destruction movement of centrifugal ball device was analysed and the conditional expression for angle of self-destroying bevel in centrifugal ball device that ensure the self-destruction firing pin movement of centrifugal ball device can not self locked were got.

为了正确设计引信离心球自毁机构自毁斜面角度，从而拓展离心球自毁机构适用的弹道环境范围并提高其自毁作用可靠性，通过分析离心球自毁机构自毁运动，得出了确保离心球自毁机构自毁戳击运动不自锁的自毁斜面角度条件式。

Methods Mononuclear cells of peripheral blood were isolated by Ficoll Hypaque centrifugation from 32 breast cancer patients and plated on 6-well culture plates(106 /ml, 2 ml/well) in RPMI1640 medium supplemented with 10％ heat-inactivated fetal bovine serum, GM-CSF, IL-4, and/or TNF-α. Two h later, nonadherent cells were gently removed and fresh medium was added.

方法采用密度梯度离心的方法，分离32例乳腺癌患者外周血中的单个核细胞，在6孔培养板上(106/ml，2ml/孔)，用含10％热灭火的小牛血清和GM-CSF、IL-4、TNF-α细胞因子的RPMI 1640培养基培养。

The changes include:(1) Put the urediniospores into the tube with two steel balls and 1. 6 g/L Chelex-100,the uredinia may be picked up with airing host tissues.(2) Add KOH into the extraction buffer instead of NaOH and vortex the tube violently for 30 min.(3) Prick a hole under the bottom instead of picking out of the steel balls, then centrifuge the tube to toss the liquid into the sheathed tube.

改进的 内容包括：(1)在有二钢珠和1.6 g／L Chelex的离心管中加入夏孢子，夏孢子可以来自带有风干寄主叶肉组织的夏孢子堆；(2)在离心管中加入KOH而不是NaOH，然后剧烈斡旋30 min；(3)将离心管管底穿一小孔，套上一新离心管，短暂离心甩出内容物。

Is the professional production of fan manufacturers, are: gas compression centrifugal blowers; boiler drum with centrifugal fan; generally low, medium, high-grade centrifugal fan, high-temperature preservation centrifugal fan; coal centrifugal ventilation ; Row chemical dust centrifugal fan; general Axial Fan; mining and energy-saving Axial Fan coal mine blast by axial fans.

禹州市鸿华机械制造有限公司是风机生产的专业厂家，主要有：煤气加压离心鼓风机；锅炉离心鼓引风机；一般低、中、高档离心通风机；高温防腐离心通风机；煤炭离心通风机；排尘化工用离心通风机；一般轴流通风机；矿用节能轴流通风机及煤矿防爆用轴流风机。

Anti-Corrosion Products of explosion-proof fans have: DWT-I FRP axial fan roof; DWT-II glass-steel roof centrifugal blower; BF4-72 C-type glass fiber reinforced plastic centrifugal fan; FBT35 FRP axial flow fan; FT30 C-type FRP axial flow fan; FRP DT Multi-wing Low Noise Centrifugal Fan; advertisement FRP fan; PVC4-62 PVC centrifugal fan; PP4-62 polypropylene plastic centrifugal fan; 4-72 stainless steel centrifugal fan; 11-62 stainless steel centrifugal fan.

防腐蚀防爆风机的产品有： DWT-I 玻璃钢轴流屋顶风机； DWT-II 玻璃钢离心屋顶风机； BF4-72 C 式玻璃钢离心风机； FBT35 玻璃钢轴流风机； FT30 C 式玻璃钢轴流风机；玻璃钢 DT多翼式低噪音离心风机；玻璃钢广告风机； PVC4-62 聚氯乙烯塑料离心风机； PP4-62 聚丙烯塑料离心风机； 4-72 不锈钢离心风机； 11-62 不锈钢离心风机。

All these could be counted as France's alienation in this crisis; in second Moroccan crisis, the pro-German group in British Cabinet opposed the diplomatic authorities of their pro-French foreign policy and asked for a more friendly relation with German, which embodied Britain's "isolation" in foreign policy in this crisis.

两次危机中反映的英法外交模式便是：在面临由德国引发的国际危机时，英法两国国内的亲德势力扮演着同样的离心的角色；而危机的解决方法无出于英国的&警告德国，但是不对法国做出任何书面承诺&。

Inflorescences centrifugal (uppermost flower or flower closest to axis opening first), usually clearly cymose, sometimes grouped into elongated thyrses or reduced to axillary flowers or clusters of flowers; stigma lobes usually linear to tongue-shaped ca

花序离心的(最上部的花或花最接近于轴开阔的的1)，柱头裂片通常线形在舌形ca

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。