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To establish an effective method of in vitro treating nonliquefying semen.

为建立一种体外处理不液化精液的有效方法,将20例不育症患者不液化精液每份分成对照组、离心洗涤组、孵育组,分别检测三组样本精子的多项功能指标。

Wear particles in the osteolytic tissue can be obtained with strong base digestion and differential centrifugation, which is up to the demand of experimental study.

通过强碱消化及密度梯度离心提取、纯化假体周围骨溶解组织中磨损颗粒的方法可提供符合试验需要的金属颗粒。

An improved purification procedure for satsuma dwarf viruswithout sucrose density-gradient centrifugation was developed,by whichglobular SDV particles about 25 nm in diameted were observed with elec-tron microscopy,using only 100-200 g of Physalis floridana-infectedleaves as the virus source.

本文提出的温州蜜柑萎缩病毒提纯法,仅用100~200克病洋酸浆叶,且无需通过蔗糖密度梯度离心,在电镜下亦能看到中量直径约25nm 的球状病毒颗粒,粗提纯制剂OD_(860)/OD_(280)平均值为1.28,OD_(260)/OD_(240)平均值为1.15,初步显示出SDV 特征性紫外吸收曲线,提纯病毒产量约为4.5~11mg/kg病叶。

When BMSCs proliferated to enough cell number (7×106). 500 000 cells were centrifuged to a pellet and cultured in chondrogenic medium supplemented with DMEM high-glucose, insulin, transferring, selectin, pyroracemic acid, 2-pyrrolidine carboxylic acid, BSA, dexamethasone and ascorbic acid for 6 weeks.

当培养的骨髓基质干细胞达到7×106个细胞时,将每500 000个细胞离心制成高密度细胞微球,在含有DMEM高糖、胰岛素、转铁蛋白、选择素、丙酮酸、脯氨酸、 BSA、地塞米松、抗坏血酸的促软骨细胞分化培养液培养6周。

When BMSCs proliferated to enough cell number (7×10^6). 500 000 cells were centrifuged to a pellet and cultured in chondrogenie medium supplemented with DMEM high-glucose, insulin, transferring, selectin, pyroracemic acid, 2-pyrrolidine carboxylic acid, BSA, dexamethasone and ascorbic acid for 6 weeks.

当培养的骨髓基质干细胞达到7×10^6个细胞时,将每500000个细胞离心制成高密度细胞微球,在含有DMEM高糖、胰岛素、转铁蛋白、选择素、丙酮酸、脯氨酸、BSA、地塞米松、抗坏血酸的促软骨细胞分化培养液培养6周。

Finally, high-speed centrifugation was applied to the conservation of crude phycobiliprotein, indicating,its effects on removement of denaturalized protein molecule with loss of phycobiliprotein.

最后研究了高速离心对脱盐液保存的影响,认为其在沉降大分子物质和变性蛋白的同时,也必然会使目标蛋白有所损失。

Finally, high-speed centrifugation was applied to the conservation of crude phycobiliprotein, indicating,its effects on removement of denaturalized protein molecule with loss of phycobiliprotein.

最后研究了高速离心对脱盐液保存的影响,认为其在沉降分子物质和变性蛋白的同时,也必然会使目标蛋白有所损失。

Resuspend bacterial cell pellet in 250 l buffer P1 and transfer to a microcentrifuge tube.

将沉淀的细菌细胞以 250 l 的缓冲溶液P1重新悬浮,并换到微量离心管。

In anisometropic and strabismic amblyopia, the abnormalities of mVEP were larger than those of mERG in almost all eccentricities.c The abnormalities of mVEP were correlated with the damage of visual acuity of the amblyopic eye in all six eccentricites.

斜视性弱视患者mVEP和:二ERG都存在中心区变异大于周边区的趋势,除第六环外,在各个离心度n1V匕P的波形异常程度均大于mERG。天津医科大学博士学位论文 3。

Novel Evidence for the Location of the PsbS protein within Photosystem ll supercomplex By sucrose density gradient centrifugation PSll-LHCll supercomplex was isolated directly from thylakoid membranes of soybean and wheat leaves and the PsbS protein was found in this supercomplex by immunoblotting with anti-PsbS antibody.

一PsbS定位于PSll超级复合体内的新证据用蔗糖密度梯度离心分离出大豆和小麦叶片的PSll超级复合体,并且在超级复合体中用PsbS抗体免疫检测到PsbS。

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This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。