离心的

Methods: rMSCs were separated from rib marrow with Ficoll Paque reagent and expanded in culture medium. Differentiation of rMSCs into neurons were induced with DMEM/monothioglycerol.

采用Ficoll Paque液(1.083 g/L)离心分离兔MSC，体外扩增，硫代甘油等试剂的无血清达乐伯克改良必需基本培养基诱导MSC分化为神经元。

Based on the project of high-embankment widening of highway, the water content and degree of compaction of foundation soil, filling and improved soil by lime were determined by means of indoor physicomechanical test. The centrifugal modeling tests were used to simulate increasing degree of compaction and improve soil by lime for coordinating deformation between new and old high-embankment widening.

针对高速公路拓宽工程高填方路段新老路堤拼接问题，通过室内物理力学试验确定地基土、素土填料及其石灰改性土在拼接路堤填筑时的含水量和压实度控制参数；利用离心模型试验手段，进行提高填料压实度和石灰改性措施下拼接路堤协调变形试验研究。

The wall-broken Cole bee pollen lOOg is extracted by 1:10 of anhydrous ethanol , continuously stirred in darkness for two hours. And then by means of centrifuge, cleaning, vacuum concentration, adjusting pH value, anion exchange resin chromatography, propanone extraction,being blown to dry and freeze-dried, we use HPLC to detect the content of the GA derivative of the freeze-dried powder.

将破壁油菜蜂花粉100g，在避光条件下用无水乙醇(料液比1：10)连续搅拌浸提2小时，浸提液经离心、洗涤、真空浓缩、pH值调节、阴阳离子树脂交换、乙酸乙酯萃取、吹干再冻干后用HPLC法分析，得冻干样品中赤霉素衍生物的含量为51.29％。

Samples of culture broth were centrifuged (3000 g, 4 C) and proteolytic activity was determined in the supernatant.

发酵液样品的离心（3000克，4 C）和蛋白水解活性在上清确定。

Inoculate a single colony in BMGY medium, grow at 30 °C with 250 r/min until culture reatch an OD6oo=2.0. Harvest the cells by centrifuging at 1,500 r/min for 5 min at room temperature. Resuspend cell pellet to an OD6oo=10 in BMMY medium to start induction.

将经过酶切鉴定和测序正确的阳性克隆电转入毕赤酵母GS115中，挑取酵母转化单菌落，接种于BMGY培养基，30℃、250r/min培养至OD_(600)=2.0时室温1，500r/min离心5min，用BMMY培养基重悬菌体使OD_(600)=1.0，重新放入摇床进行诱导。

Chinese scientists have developed a method to separate mixtures of metallic and semiconducting single-walled carbon nanotubes by a two-step dispersion-centrifugation process.

我国科学家已经开发出一种方法，独立的混合金属和半导体单壁碳纳米管由两步分散离心过程。

Methods 614 sputum samples were detected by centrifuging the liquored sputa and collecting and normal smear method simultaneously.

对614份痰标本同时进行夹层杯集菌离心涂片法与普通痰涂片法检测抗酸杆菌，比较2种方法的检出率。

Results The positive rate of centrifuging the liquored sputa and collecting bacteria was 32.28%, it is significantly higher than the normal smear method (19.87%).

夹层杯集菌离心涂片法简单省时、阳性率高，且便于进行质量控制，是一种很有推广价值的检测方法。

The abnormality of the mVEP waveform in amblyopia decreased with the eccentricity increased, such changes in mERG were only found in strabismic amblyopia.

在所有离心度mVEP波形异常私i度均大于mERG，但在中心区两者的差距更明显。

The optimal conditions were 3 %B+1.5 %A ⅢZTC1+1,pH 5.5 and 3 000 r/min 10 min.The transmittancy of clarified beverage was over 98 % and the beverage could be long stored.

最佳工艺条件为：ZTC1+1Ⅲ型澄清剂添加量3%B+1.5%A、pH5.5、离心分离3000r/min10min，所得西洋参浸提液透光率达到98%以上，制得的保健饮料可长久贮存。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。