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More than 95% purity achieved after removal erythrocyte, nonparenchymal cells and dead cells with multi-step low-speed centrifugalization.

通过多步低速离心可去除红细胞、其他非实质细胞和死亡的肝细胞,其纯度可达95%以上。

Methods: Biological technique, including centrifugalization, ion-exchange chromatography, hydrophobic chromatography and dialysis was used. Osteopontin from bovine milk were identified by SDS-PAGE and Western Blot.

运用生物化学技术,通过离心脱脂后,再经过离子交换层析、疏水层析、透析等技术达到分离纯化目的;随后对分离纯化得到的产物进行SDS-PAGE电泳、免疫印迹技术鉴定。

Results: The recovery rate of the bacterial put into drug before centrifugalization was evidently different from that after the last washing of membance filtration.

结果:离心前加入阳性对照菌所得回收率非常低,采用薄膜过淋法的阳性对照菌所得回收率可达70%以上,两者有显著性差异。

After two weeks take the blood serum through centrifugalization the blood obtained from mice,and determine the antibody production level in serum with ELISA compared with standard serum.

采用决明子20%水煎提取液分别给小鼠进行口服灌胃及腹腔内注射,同时设立对照组,采用生理盐水分别给小鼠进行口服灌胃及腹腔内注射。2周后取小鼠血离心后取血清,用ELISA法测定血清中抗体产生的水平,并用标准血清进行对照。

Platelet-rich plasmais obtained from autologous blood after centrifugalization in a cell separator.Originally,PRP was used in clinic as a biologic plasma and barrier velum,in order to enhance the effect of hemostasia.

中文摘要:富血小板血浆是自体血离心后的产物,其最初被作为一种生物凝胶和屏障膜应用于临床,主要作用为提高止血效果。

Adding Ficoll to centrifugalize, BMSCs were isolated, cultured and proliferated. The intestinal segment about 15 mm×5 mm was obtained sterilely from fetal duodenal papilla to colon, then muscular tunic and adventitia were peeled.

收集四五个月流产胎儿标本6例的四肢骨髓,Ficoll离心,分离、培养、扩增获取骨髓间充质干细胞。

Homogenize, collect supernatant after centrifugate at 3000rpm speed.

然后以3000rpm的转速离心10min,吸取上清液。

Animals after chemolysis failure to homogenate respectively,centrifugate and take the pure liquid,mcasure collagenase activity with Blanckaert method,the data handled with statistics did not show obvious difference.

组织化学检测:将实验动物组、对照组、手术组和化学溶解术失败再手术组标本的纤维环内层、中层和外层分别匀浆、离心,取上清液,按Blanckaert法测定胶原酶,所得据经统计学处理差异无显著性。

Methods By using to turn differently to carry on centrifugence to the same batch of specimen with time soon,and then sing ELISA examination HBsAg.

用不同的转速和时间对同一批标本进行离心,再用ELISA法检测乙肝表面抗原。

After ultrasonic extraction, centrifugence and concentration, the samples were finally determined by high-performance liquid chromatography.

采集的土壤样品经超声萃取、离心、浓缩以后用高效液相色谱测定其含量;灭螺试验分别计算3 d、7 d钉螺死亡率。

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