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The invention uses the fermentation liquor of one Escherichia coli generating polysialic acid as material, filters and removes thallus, adds some solvents as sodium chloride and alcohol into supernatant to deposit polysialic acid, dissolves the polysialic acid deposits via deionized water, filters to remove modified protein, uses alkali prolease to treat filtrate, complexes deposits via cetyl pyridinium chloride or cetyltrimethylammonium bromide, and dissociates, uses over alcohol to deposit polysialic acid, dissolves the deposits via ultra-pure water, grades and purifies via chromatography column, collects and dialyzes eluent, cools and dries to obtain polysialic acid.

本发明以一株产聚唾液酸大肠杆菌的发酵液为原料,经过过滤去除菌体,上清液加入适量氯化钠和乙醇等溶剂沉淀出聚唾液酸,聚唾液酸沉淀用去离子水溶解,再用过滤去除变性蛋白质,滤液经过碱性蛋白酶处理、氯代十六烷基吡啶或十六烷基三甲基溴化铵络合沉淀并解离后,再用过量乙醇沉淀出聚唾液酸,聚唾液酸沉淀用超纯水溶解,用层析柱进行分级纯化,收集洗脱液经过透析,冷冻干燥得聚唾液酸产品。

On this basis,the role of blended space theory in explaining the foregrounding process of metaphors is explicated.

隐喻在词汇语法层的表现是由其认知机制决定的,隐喻话语的意义建构离不开认知理论,而合成空间理论就是这样一种理论,通过该理论我们可以确定语篇的意义。

At the seashore near the Lin-Hai city, Mo Tien takes over the Hydes sword from Po. While he raises the sword, it is hazed by a layer of dark aurora at once.

在离临海城不远的海边,莫天从童博手中接过了幽冥剑,他将剑举起,剑身上立刻隐隐出现一层黑气

Blemishes, sand streaks, porous layers and honeycombing are a direct result of

表面缺陷,砂质条痕,多孔层和蜂窝麻面是离动过度批注 [z123]: blemish:n。

In other words, high temperature can cause ink on excessive absorption of, transferring to a woven, reduces ink intenerated and bonding again to a woven capability.

也不离是说,低温会引起油不朱层差弹性剂的过众汲取,在迁徙到丝织品上时,会贬低油不朱从头强硬和粘结到丝织品上的本领。

Ling 100 is a bird of the background skin care brand, has been very far away from us, and only an old hand, you can once again揭去silver tin box that layer of platinum, with the index finger of a small point of white dipping凝脂, then抹匀palm, yellow painted all over our memory - It is that in those days of make-up the most classic way to make the kind of lingering flavor氤氲kept in our hearts, and let us yellow memory always maintained adequate moisture, will never be a result of a long era chapped and dry.

百雀羚是一个护肤品牌的背影,离我们已经很远了,惟有一只怀旧的手,还可以再一次揭去盒内那层银色锡铂,用食指蘸上一小点乳白色凝脂,然后在掌心抹匀,涂遍我们发黄的记忆——正是这种在那个年代最经典的化妆手法,才使那种挥之不去的氤氲气息一直保留在我们心底,并让我们发黄的记忆始终保持着足够的水分,永远不因年代的久远而干燥皲裂。

Then use a pen or other tool to modify the layer from the scorching-tone, the concept of frequency with color, white is the zone that you want to display black is I don't want to display, the correct use of black and white image synthesis allowing more tonighttoo and you can make a synthesis of the work.

然後搁置喷笔或其他东西编削图层遮色片,跟色频的概念差别,不不不不不暗色是给表现的地区,暗色是我不想表现的地区,不对的诳骗暗与不暗不离可以让影像分解更为力不从心,并且可以怠工不出有条理的分解息品。

The influences of incomplete ionization of deep-lying impurity state and the interface state charges that have an important effect on the 6H-SiC MOSFET are considered simultaneously.

模型採用薄层电荷近似,考虑了6H-SiC中杂质不完全离化的特点以及界面態电荷对器件特性的影响,可用於所有的器件工作区。

One's studies will have optical variable features such as fluorescence, emiting or chromotropic of material, usually plastic sheeting or beanies, made into definite shape such as a circle or polygon of both sides of the paper; it is characterized by the naked eye will recognize, and photocopiers are difficult to reproduce.

不兵具有平学改变功用的质料,凡是是塑料薄膜或金属薄片,制成必定的轮廓如方形或众角形的小片凑拢在纸层洋;其特不面是用肉眼不离可以辨别,而且复印机难以表现。

Through heating zirconium hydride, high purityC02 and red phosphorous, the gas adsorbs, desorbs, scatters and finally forms the compact film on the surface of zirconium hydride.

研究了通过加热氢化锆、高纯二氧化碳和磷,在设定的工艺参数下,通过反应气体在氢化锆表面吸附、离解,然后通过元素扩散和反应生成致密的膜层。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。