离子载体

Methods Cadmium-specific promoter PcadA was obtained by PCR from pPcadA and was ligated to E.coli-B.subtilis shuttle vector pNGFP with gfp being reporter gene ,after which the recombinant plasmid was transformed into 1A751 strain to form the bio-engineered bacteria.

采用聚合酶链式反应从质粒pPcadA上扩增得到重金属镉离子特异性启动子PcadA，以绿色荧光蛋白基因为报告基因，以大肠杆菌-枯草杆菌穿梭载体pNGFP构建了绿色荧光蛋白镉离子诱导表达载体。

Additional, in industrial respect, ion exchange fiber can become activator carrier as activator, use at catalysis certain and organic reaction; Can use as dehydrating agent and grease depart material; Use as water sorbent, take off with what divide at the water in storehouse except; Use as material of sex hygiene suction blood, can make radioactivity is absorbed and defend material; Use ion exchange fiber the adsorption to the bacterium and antiseptic ability, make underwear of deodorant insole, health care wait.

另外，在工业方面，离子交换纤维可作为催化剂成催化剂载体，用于催化某些有机反应；可用作脱水剂和油水分离材料；用作水吸附(物质表面吸住周围介质中的分子或离子现象)剂，用于仓库中水分的脱除；用作吸血性卫生材料，可制成放射性吸收和防护材料；利用离子交换纤维对细菌的吸附(物质表面吸住周围介质中的分子或离子现象)和杀菌能力，制成防臭鞋垫、保健内衣等。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下：1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草，获得三个单基因和一个双基因的RNAi沉默表达植株，快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加；叶组织内Na+离子含量和K+离子含量均略高，但差异不显著；K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降，但差异不显著；K+离子含量比对照显著降低。

As to MNa-ZSM-5, the order is CuNa-ZSM-5〓AgNa-ZSM-5＞CeNa-ZSM-5＞ CoNa-ZSM-5〓FeNa-ZSM-5＞Na-ZSM-5. Among the samples tested, CoHZSM-5 shows the highest activity, the conversion of NO is about 60%, and the activity is higher than that of CoNa-ZSM-5 with the same exchanged level. Suggests that the exist of some Bronsted acidity is benefited for this reaction. For Fe-ZSM-5, the activity are changed obviously with the various of preparation condition. It was also found that mordenite zeolite is a good support for this reaction.

其中，Co-HZSM-5表现出最佳的反应活性，NO的最高转化率达60％以上，并且活性明显高于具有相同交换度的CoNa-ZSM-5，表明适量的酸中心存在有利于该反应的进行；对于Fe-ZSM-5，制备条件不同Fe-ZSM-5的反应活性影响很大，反应的活性中心应是处在交换位上的铁离子；另外，通过考察不同载体对铁离子交换分子筛催化剂反应性能的影响，发现丝光分子筛也是较好的催化剂载体。

Ethanol, ionophore A23187 and electrical stimulations all induced 〓 rises in oocytes.

乙醇、钙离子载体A23187和电刺激均可使胞质游离Ca〓浓度升高。

Objective To investigate the effect of assisted oocyte activation with calcium ionophore A23187 and puromycin on human oocytes that fail to fertilize after intracytoplasmic sperm injection.

目的观察钙离子载体A23187联合嘌呤霉素对卵母细胞质内单精子注射后未受精卵母细胞的激活作用。

On the other hand, epidermal growth factor , calcium ionophore (A23187), kallikrein and tumor promoter (phorbol-12 myristate-13 acetate) have been shown to stimulate the synthesis of prostaglandins from the thymic epithelial cells.

在另一方面，表皮生长因子、钙离子载体（A〓3817）、激肽释放酶和肿瘤启动子却能促进胸腺内分泌上皮细胞中前列腺素的合成。

A novel lead ionselective polyvinyl chloride membrane electrode was prepared using 25,27di(2thiadiazolethiooxyethyl)26,28dihydroxyl5,11,17,23tetraptertbutylcalix[4]arene as the ionophore. The influences of the nature of plasticizers and the amounts of the incorporated ionophore and ionic additive on the response characteristics of the electrode were studied.

制备了以25，27二(2噻二唑基硫代乙氧基)26，28二羟基5，11，17，23四叔丁基杯\[4\]芳烃为载体的聚氯乙烯膜铅离子选择电极；研究了电极膜中增塑剂种类及载体和离子定域体含量对电极性能的影响。

A denitrifying oxide catalyst contains one prous metal carrier, ZSM-5 zeolite directly crystallized on said carrier and one metallic iron chosen from IB family or VIII family or their mixture. Said porous metal carrier contains at least one of prous Ni-Al, Fe-Al or Cu-Al alloys.

一种含ZSM－5沸石的脱氮氧化物催化剂含有一种多孔金属载体和直接晶化在该载体上的ZSM－5沸石和一种选自ⅠB族、Ⅷ族的金属离子或其混合物，所述多孔金属载体至少含有一种多孔镍－铝、铁－铝或铜－铝合金。

Methods Explore the optimal concentration of phurbol-12-myristate-acetate and calcium ionophore (A23817) inducing time, and optimal concentration of biotinyl sheep anti mouce IgG (bio-SAM-IgG) and horse radish peroxidasestreptavidin. The CD4(superscript +) cell enriched from peripheral blood mononuclear cells using solid phase anti-CDs monoclonal antibodies were induced by PMA and A23817 under influence of monensin and harvested in vary hours.

从健康人外周血单个核细胞中富集CD4细胞，加入莫能霉素，用不同浓度的佛波醇酯和钙离子载体(A23817)诱导CD4细胞不同时间，比较其胞内表达白细胞介素－2(IL-2)/白细胞介素－4(IL-4)的细胞百分率，确定最适诱生条件；用不同稀释度的IL-2McAb、IL-4McAb、不同浓度的生物素化羊抗鼠IgG(bio-SAM IgG)和辣根过氧化物酶标记的链霉亲和素作棋盘配比试验，确认McAb、bio-SAM IgG和HRP-SA的最适浓度，根据上述结果建立了检测细胞内细胞因子的BSA-ICC法。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。