离子结合

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

Based on the theory of transnormal ferrimanganic materials, the water quality treatment technique to this kind of drinking water are discussed with the example of unsafety status for drinking water in Liaoning rural area. The merits and demerits of traditional oxidation method and ion exchange method are compared and suitable method for the rural area is put forward.

根据地下水铁锰超标的机理，结合辽宁省农村饮水不安全现状及农村饮水工程现状，探讨铁锰超标饮用水的水处理技术，比较传统的氧化法除铁锰技术与离子交换法除铁锰技术的优缺点，通过实际应用，研究适宜于农村饮用水的水质处理技术。

According to Johnson's Rule and IR spectra, their possible structures are suggested. The bonding parameters of those complexes are calculated by ESR parameters. And the crystal field parameters are calculated by using electronic spectral data. It can be seen that the vanadyl complexes in the solution are ionic complexes and that the α〓 values of those complexes decrease following the increasing of the N donor replacing the O donor of water on the equatorial plane in those complexes. This means that the covalent bonding between vanadium and ligand increases as Ser, Phen or Bipy replace water ligands. The results show the competitive coordinate law that the coordination reactivity of Phen and Bipy is much stronger than that of Ser. The simulation program of ESR is compiled by the theory of ESR in the present work.

测定了VO-丝氨酸二元体系、VO-丝氨酸-邻菲咯啉三元体系和VO-丝氨酸联吡啶三元体系在不同酸度下（pH=1.0～14.0）的乙二醇／水（V／V=1：1）溶液低温ESR谱，发现不同pH下，溶液中具有不同组成的配合物，根据不同pH下ESR谱的变化，利用Johnson的加合规则并结合红外光谱，推测了它们在溶液中的可能结构；利用波谱参数计算了配合物键参数；利用电子光谱数据计算了配合物的晶体场参数，讨沦了它们的成配特性，结果表明：VO配合物属离子型配合物，随取代水分子的配体（Ser、Phen或Bipy）数目增加，键参数减小，配合物共价性增强，同时得出这些体系中，生物配体竞争配位规律：Phen和Bipy与VO的配位能力比Ser强。

Metallothioneins are a group of cysteine-rich, low-molecular-weight proteins, which can bind metal ions through mercaptide bonds.

金属硫蛋白(metallothionein，简称MT)是一类富含半胱氨酸的低分子量蛋白质，能够通过配位键结合金属离子。

A new glass coating technology to combine the method of ionic pervasion colouration with the technique of chemical vapour deposition was adapted.

将离子渗透着色技术和化学气相沉积技术加以结合，在浮法玻璃生产线的锡槽内和AO区进行2次镀膜形成复合膜，克服了单一镀膜技术存在的不足。

The synergistic effect of Cu and Mn is listed as follows:(1) On improving the structure of rust layer, addition of Cu and Mn can reduce cracks and pores in rust layer;(2) On altering the composition of rust, it can increase the amount of Fe3O4 and γ-FeOOH, while decrease that of β-FeOOH;(3) On the chemical state of alloying elements and selectivity of rust layer, it mainly convert the anion selectivity to canoed selectivity;(4) On the electrochemical aspect, it can decrease the anode current density and increase the corrosion potential of steel.

Cu、Mn的协同作用主要体现在以下几个方面：在改善锈层结构方面，主要是减少锈层中的裂纹与孔洞，提高锈层与基体的结合强度；在改变锈层组成方面，增加Fe3O4和γ-FeOOH的含量，降低β-FeOOH的含量；在元素化学状态与离子选择性方面，促使锈层由阴离子选择性转变为阳离子选择性，抑制Cl-的渗透；在电化学方面，大幅度降低阳极电流密度，提高钢基体的自腐蚀电位。

According to the experimental and calculation results, the enthalpies of formation of the ions and some bond energies have been evaluated.

结合实验和理论计算结果，估算了离子的标准生成焓和键解离能。

But, alpha-synuclein titrated with Fe revealed a K of 10(5)M with single binding site.

用铁离子滴定阿尔法-突触核蛋白只发现一个结合部位，10(5)。

The effects of the configuration between the metal ions and BSA on the...

讨论了金属离子与BSA的结合构型对BC与BSA相互作用的影响。

In the coarse size range, calcium ions were the largest, and the water extract of the coarse particles showed alkaline reaction.

结果显示：在广州大气粒子中，NO3-主要存在于粗大粒子中，而且和其他物质结合在一起；在粗大粒子中，溶液呈碱性，而且钙离子的含量最大。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。