离子结合

objective: to achieve the soluble expression of mn-sod gene in e.coli and assay the enzyme activity of the expressed product. methods: the coding region of superoxide dismutase was amplified using pcr method from the e.coli genome. the pcr product was cloned into puc19-t vector and sequenced.in addition,the cloned coding region of mn-sod was inserted into the expression vector pet-28a to form the recombinant plasmid pet-28a-mn-sod and was then transformed into e.coli bl21 for expression.

超氧化物歧化酶(superoxide dismutase，sod)是细胞体内歧化超氧阴离子自由基(o-2)的一个抗氧化酶，按其结合的金属性离子根据其中金属辅基的不同可分为4类：cu/zn-sod、mn-sod、fe-sod和ni-sod，它们通过催化超氧阴离子自由基0-2发生歧化反应，达到清除o-2的效果，具有防御氧毒性、增强机体抗辐射损伤能力、防衰老以及治疗某些肿瘤、炎症、自身免疫疾病等功效，广受国内外科研工作者的关注和重视[1]。

The slow sodium influx educed by veratridine and the calcium-dependent potassium outflux are regarded as the two slow variables, which are responsible for the parabolic bursting.

结合实验模型，从离子通道活动的角度揭示了抛物线簇放电发生的生物物理机制。由藜芦碱诱发的慢变钠内流和钙依赖钾外流被认为是引发实验所观察到的抛物线簇放电的两个慢变量。

For this reaction, there may be two consecutive steps-Adrenaline→Adrenochrome→other products.

利用紫外吸收光谱法结合计算机模拟研究了肾上腺素在Cu离子催化下，用H_2O_2氧化产生肾上腺素红及其进一步反应的动力学。

In this work, a new process based on ion-exchange and high vaccumn distillation was developed. A weak alkalic ionexchange resin, D301, was screened out.

本文研究开发了以离子交换树脂法和真空精馏结合分离提纯乙酰丙酸的新工艺。

The results revealed that iontophoresis could significantly improve the penetration of SLN. The fluorescence of images indicated that the accumulation and the depth of QDs-SLN reached in skin increased with times. Anodal delivery of QDs-SLN resulted in a higher accumulation of QDs-SLN in skin than those of cathodal delivery. Furthermore, the vertical images of skin showed that fluorescence assemble in follicle and dermis, indicating that follicle and the junctions between the corneocyte clusters provide the pathway for SLN penetration into skin.

QDs-SLN在皮肤中的滞留量及透皮深度随离子导入时间的增加而增大；正极导入时，QDs-SLN在皮肤深层组织中的含量较高；通过荧光显微镜对垂直于皮肤表面的纵切猪耳全皮中QDs-SLN的分布情况进行考察，结果提示皮肤附属器及角质层中蛋白簇间的结合部位可能为SLN的透皮路径。

Finally, SO2 removal efficiency empirical equation in the scope of experimental condition is gained from the result of uniform experiments by means of stepwise regression analysis.3. Under the basis of the experimental results in lab, crossflow packed-bed absorber for additional experiment is designed according to experience. The dimension of packed-bed absorber is fixed and flooding gas speed of absorber is checkouted. At the same time, calandria liquid distributor is designed. The efficiency of flue gas desulfurization by scrap iron is investigated in the additional equipment. Under th

为了更好的掌握脱硫工艺的过程规律，根据脱硫工艺涉及的Fe浓度范围，选用NaHso3作为反应物质，全面研究10一6M、阵扭l：IM范围内Fe液相催化氧化s反应动力学，将Fe浓度分为两个范围对实验结果加以讨论，确定HSO3一和Fe的反应级数，调查离子强度和温度对反应速率常数的影响；并结合研究者提出的自由基机理和反应历程，分析反应机理，解释动力学规律。

A carbonium ion mechanism combined with a bimolecular intermediate mechanism may be explained the isomerization of n-hexane on Pd/H-mordenite.

对正己烷在Pd/HM上的反应过程可用正碳离子机理结合双分子六元环中间物机理解释。

The invention discloses a synthetic using method of smelting high-furnace dust, which comprises the following steps: grinding the high-furnace dust with 1.5-15% zinc; proceeding magnetic cobbing; reclassifying to obtain the refined ferric ore; disposing ore tail through waste acid of titanium dioxide with 15-30% sulphuric acid; transmitting the zinc oxide in the solution at zinc sulfate pattern; separating solid and liquid solution through lime; obtaining refined zinc ore through S2-, CO32-, HCO3- ion; floating the immersed slag through neutral oil.

一种炼铁高炉炉尘资源综合利用的方法，含锌1.5％～15％的高炉炉尘经磨矿、磁选、重选回收铁矿物获得铁精矿，选矿尾矿用含硫酸15％～30％的钛白废酸处理，使其中的氧化锌以硫酸锌的形式转入溶液中，固液分离得出的溶液用石灰中和除铁后，再用S2-、CO32-、HCO3-离子沉锌获得锌精矿，浸出渣用中性油浮选获得炭精矿。该方法将钛白废酸和高炉炉尘结合在一起，使之成为互为所需的原料和试剂，有效地将两种废弃物变成了有价资源，较好解决了环境污染问题，同时又能产生好的经济效益。

CuRF coupounds are based on the combination of deprotonation and ICT mechanisms. The secondary amines conjugated to 1,8-naphthalimide could be deprotonated by Cu2+, and as a result, large red shifts in both absorption and fluorescence spectra were obtained, from which one could sense Cu2+ colorimetrically and ratiometrically.

CuRF 系列则是基于脱氢和ICT原理的结合，铜离子与受体的作用能够诱使共轭氮原子上的氢脱去，共轭氮原子显负电增强了对荧光团的供电能力，从而引起荧光波长的红移，同时也造成了吸收光谱的红移。

A simple and sensitive method was investigated for simultaneous determination six organic acids in wine (tatartic acid, citric acid, malic acid, formic acid, acetic acid and succinic acid) by single ion-exclusion chromatography with conductometric detection.

用单柱离子排斥色谱法分离结合电导检测同时测定了多种有机酸（酒石酸、柠檬酸、苹果酸、甲酸、乙酸和琥珀酸）。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。