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The extracts of water and alcohol were separated by ion exchange resin. The eluates of neutral solution were the most effective against hydroxyl radicals and the highest rate of inhibiting hydroxyl radicals was 81%. The eluates of basic solution were also effective, the inhibit rate of which was 60%.

将发酵灵芝粉的水和醇提取物上离子交换树脂柱,依次用酸性、中性、碱性淋洗液洗脱分组,发现中性洗脱部分对羟基自由基抑制作用最强,抑制率最高可达81%;碱性洗脱部分亦具有抑制羟基自由基的效果;酸性洗脱部分抑制羟基自由基的作用不强,且占很少比例。

Third, the phenyl aldehyde precipitation method and ion-exchange method were utilized to refine the eluent.

次之,分别采用苯甲醛沉淀法和离子交换法对L-精氨酸粗提液进行纯化精制。

For each pair of amino acids, determine which will be eluted first from the ion-exchange column using a pH 7.0 buffer.

确定下列每对氨基酸在pH7.0的缓冲液中,哪个先从离子交换柱上洗脱下来。

Aminobutyric acid in tea was extracted, isolated and determined. Water achieve a better effect than ethanol in extracting γ-aminobutyric acid, and γ-aminobutyric acid content was enhanced by 20%; 732 positive ion resin columns (2.5×50cm) was used to isolate, citric acid and ammonia water were used as elutes, based on pH linear gradient. Results indicated that γ-aminobutyric acid inelute could reach 39%; thin layer scanner could measure the content of γ-aminobutyric acid.

对茶叶中γ-氨基丁酸的提取、分离及检测方法进行了研究,分别采用水和乙醇作为提取溶剂,结果发现用水提取能达到更好的效果,γ-氨基丁酸含量提高20%;同时采用离子交换树脂对γ-氨基丁酸提取液进行分离,提取液上732阳离子树脂柱(2.5 ×50cm),使用柠檬酸和氨水缓冲液进行pH线性梯度洗脱,洗脱液中γ-氨基丁酸最高浓度达39%;采用薄层扫描仪对γ-氨基丁酸的含量进行检测,研究并确定了检测的条件及参数。

Dorsalis by salting out, ion exchange chromatography, and gel filtration chromatography. By salting out the egg homogenate, SDS-PAGE analysis revealed that the Vns were mainly precipitated in a 50~70% ammonium sulfate solution. The salted-out proteins were further purified by ion exchange chromatography, and the result showed that Vns were mainly eluted with a 0.3~0.5 M sodium chloride gradient. Finally, the elutes of ion exchange chromatography were refined by running them through gel filtration chromatography to obtain purified Vns. SDS-PAGE separated the 48- and 51-kDa purified Vns, and used them as antigens to raise polyclonal antibodies.

利用硫酸铵盐析法初步分离果实蝇卵中的蛋白质,以SDS-PAGE蛋白质电泳分析得知,卵黄蛋白主要在硫酸铵溶液浓度为50~70%时被沉淀出;盐析沉淀所得之卵黄蛋白,再以离子交换层析法进一步纯化显示,卵黄蛋白主要会在0.3~0.5 M氯化钠的浓度范围间被析出来;再将析出液浓缩后,经凝胶过滤层析法做最后的纯化,即获得纯化之卵黄蛋白。

In view of △F, the natural tendency of acidification as a result of aluminium transformation in red soils was further proved in thermodynamics, and the energy characteristics for exchange-adsorption of aluminum and potassium by calcium ion was revealed.

从热力学角度进一步论证了红壤铝质化即酸化的自然趋势,并揭示了钙与铝、钾离子交换吸附的能量特征。

At higher temperature Na+ can go into the channel of feldspars. The content of Na2O in feldspar increases up to 15.9 percent.

在高温条件下Na 与钾长石发生离子交换反应,能形成Na2O含量达15.9%的钠长石。

To detect recombinant MUC1 with histag by flat-bed isoelectric focusing and provide reference for purification of recombinant MUC1 without histag by ion exchange chromatography.

目的采用薄层等电聚焦方法检测含有组氨酸尾的粘蛋白表位疫苗(简称重组MUC1),为不含有组氨酸尾的粘蛋白表位疫苗的下一步离子交换层析纯化蛋白提供参考依据。

To detect recombinant MUC1 with histag by flat-bed isoelectric focusing and provide reference for purification of recombinant MUC1 without histag by ion exchange chromatography.

目的 采用薄层等电聚焦方法检测含有组氨酸尾的粘蛋白表位疫苗(简称重组MUC1),为不含有组氨酸尾的粘蛋白表位疫苗的下一步离子交换层析纯化蛋白提供参考依据。

The enzymes were furth er separated and purified into 12 components after DEAE-32 chromatography and p reparative electrophoresis.

经DEAE-纤维素离子交换和制备电泳进一步分离纯化,得到12个单一组分。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。