离体酶

The expression of sesquiterpene cyclase activity in pepper leaves were due to the induction of transcription of sesquiterpene cyclase gene by elicitors treatment.

结果表明：在CuCl2 、HgCl2 、NaCl、紫外线诱发作用下，离体辣椒叶片均能表现一定的倍半萜环化酶活性，而CK中几乎检测不出倍半萜环化酶活性。

During dehydration, the tolerance of wampee axes to desiccation which was analyzed as germination percentage, vigour index, and rate of electrolyte leakage, and activities of antioxidant enzymes gradually decreased, the content of malondialdehyde , progressively increased.

黄皮种子离体胚轴在脱水过程中，胚轴的萌发率、活力指数、电解质渗漏速率，超氧化物歧化酶、过氧化物酶和过氧化氢酶活性逐渐降低，膜质过氧化产物MDA的含量不断增加。

However, the area is less studied than other areas of endophyteresearch world wide. Therefore, a series of experiments were carried out in laboratory,glasshouse and field to compare the responses of endophyte infected plants andendophyte free plants of perennial ryegrass varieties to 10 fungal pathogensisolated from turf type ryegrass in Lanzhou. The pathogens used in the experimentswere Alternaria alternata、Bipolaris sorokiniana、Fusarium avenaceum、Curvularialunata、Ascochyta leptospora、Fusarium acuminatum、Fusarium chlamydosporum、Fusarium oxysporum、Fusarium solani and Gliocladium roseum. In addition, someprotective enzymes activity and alkaloids contents of E+ and E- plants were measuredand LpsA gene of the common strain - Lp19 and mutant strain -AR1 of Neotyphodiumlolii were sequenced to identify their ...

本研究在初步明确兰州市坪用黑麦草内生真菌及其病原真菌多样性的基础上，在实验室、温室和田间开展了系列研究，比较了带内生真菌和不带内生真菌的黑麦草在离体和整株条件下对细交链孢、离蠕孢、燕麦镰孢、新月弯孢、小孢壳二孢、锐顶镰孢、厚垣镰孢、尖镰孢、腐皮镰孢和粉红粘帚霉等10种病原真菌的抗病性，测定了E+和E-植株数种保护酶活性及其生物碱含量的变化。

By changing temperature and pH, the amylase, protease and lipase of Perca fluviatilis were studied in vitro.

研究了离体状态下河鲈体内淀粉酶、蛋自酶和脂肪酶在不同温度和pH时的活性变化。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

The calf intestinal epithelial cells in vitro were selected to evaluate the trophic actions of insulin, IGF-J and peptic and tryptic hydrolysates of bovine colostrum. The cell proliferation, glucose absorption and specific activity of cell membrane enzymes were investigated.

为研究乳源性生长因子对动物胃肠道生长发育的作用，本试验以离体犊牛小肠上皮细胞为试验对象，研究了胰岛素、胰岛素样生长因子Ⅰ和酶解牛初乳对细胞增殖、葡萄糖吸收和细胞膜酶活性的影响。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型，取健康大鼠每日灌服不同剂量的鲜姜有效部位(200，400，800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1)，取含药血清作为受试药物，用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力；逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平；酶联免疫吸附法测定MCP-1蛋白表达水平。

Using a long-term reared housefly population which is very susceptible to pyrethroid insecticides as the susceptible strain, a population collected in 1993 in Shanghai and has been treaded by cyhalothrin as the resistant strain, we bioassayed their susceptibility to organophosphorus, carbamate, pyrethroid and organehloride insecticides. We also investigated the role of metabolic enzymes, i. e. esterase, GST, MFO and of target enzymes, i. e. AChE and 〓-ATPase in resistance to insecticides. Cyhalothrin resistance inheritance, the co-toxicity coefficient of cyhalothrin and methylene dithiocyanate was also studied. The insecticides resistance in adults and larvae of houseflies was compared and the mechanism of resistance differences between adults and larvae was discussed.

本文以采自野外并经过室内多代筛选的三氟氯氰菊酯抗性家蝇品系和室内多年饲养的敏感家蝇品系为试虫，测定了抗性家蝇品系和敏感家蝇品系对拟除虫菊酯杀虫剂三氟氯氰菊酯、氯菊酯、有机磷杀虫剂DDVP、氨基甲酸酯杀虫剂残杀威、有机氧杀虫剂DDT的抗性水平，并通过活体增效剂实验和离体生化实验研究了抗性品系的解毒酶和靶标酶的变化，本文还研究了三氟氯氰菊酯和增效剂I对抗性家蝇的增效作用及增效机理、家蝇幼虫和成虫对杀虫剂的抗性差异及其抗性差异的机理。

The ABA raised activity of protease, RNase, DRNase in detached leaf and speeded up hydrolysis of protein and nucleic acid, loss of enzyme protein of the RuBPcase. The Zeatin was in apposition and antagonised negative effects of the ABA.

ABA可刺激离体叶片中蛋白质水解酶RNase、DRNase活性的上升，加速蛋白质和核酸的水解，特别是RuBP羧化酶蛋白的丧失，Zeatin则相反并且抵消ABA的负作用。

By using competitive adsorption of activated carbom to remove unfavorable influence of carbon in carbonaceous gold concentrate on leaching process, the leaching rate of gold concentrate can be improved to 88% as compared with 10%by direct cyanidation.

本文论述了利用活性炭竞争吸附作用，消除含炭金精矿中炭在浸出过程中的影响，使金精矿的浸出率可由直接氰化的10％提高到88％。

The only thing you're ever gonna be king of is King of the Stupids!

你只配做傻瓜的国王！