离体酶

The effects, of protection and treatment with, combined Ch, NaF and a trophie were significantly better than that with atropine alone. In isolated phrenic nerve-diaphragm preparation of rat, isolated jejuneal preparation of, guinea pig, and the metabolic experiment of rat liver, the results of the, assay on the rat blood ChE activity indicated that antidotal effect of NaF may be due to the antidese...

大鼠离体膈神经膈肌、豚鼠离体空肠和大鼠肝脏代谢实验及对大鼠全血胆碱酯酶活力测定的结果表明，NaF的抗毒作用可能是由于其对神经肌肉接头和/或神经节中的N受体的抗脱敏作用；而Ch则可能通过其对ChE的保护和/或加速被抑制ChE的复能，阻碍Ach到达M受体部位而发挥作用。

UV-B-induced damage in such leaves could be reduced with 0.5 mmol.L-1 NaHSO3 treatment; brown spots on the leaf surface were reduced in number, the degree of leaf wilt was reduced, degradation of chlorophyll and carotinoid was reduced and flavonoid level was increased. With the NaHSO3 treatment, the content of H2O2 in leaves of Phaseolus vulgaris under enhanc ed UV-B radiation was significantly lower than in controls, and the activities of antioxidant enzymes were higher with treatment than in controls.

结果表明：与未处理对照相比较，用0.5 mmol.L-1 NaHSO3处理的离体红芸豆叶片表面褐色斑减少、边缘蜷曲及萎蔫程度降低；且能延缓叶片中叶绿素和类胡萝卜素含量的降低；使类黄酮含量升高；叶片中过氧化物酶和抗坏血酸过氧化物酶活性升高，过氧化氢(H2O2)含量降低。

On the basis of this system andpreviously morphological, physiological and biochemical studies on cucumber in vitrogynogenesis. Materials from both typical gynogenesis using M99 as induction medium andnon gynogenesis using W5 as induction medium were collected. The activities anddistribution of peroxidase and its isoenzymes, content of soluble protein and distribution of allprotein during early stage of cucumber in vitro gynogenesis and non gynogenesis wereinvestigated by biochemistry and histochemistry technology.

本试验在前期有关离体黄瓜雌核发育形态学和生理生化研究的基础上，以诱导离体雌核发育的 M99 培养基上和非雌核发育的 W5 培养基上培养的黄瓜未受精子房为试材，采用生物化学技术和组织化学技术，深入研究了离体雌核发育早期外植体内的标志酶——过氧化物酶及其同工酶的活性及分布的变化、可溶性蛋白质的含量和总蛋白质分布的变化及核酸和多糖分布的变化，为揭示离体雌核发育早期的细胞分化机制提供科学依据。

It is Concluded that the mode of action of pyrimidine benzylamine differs from the other typically commercial ALS inhibitors, such as sulfonylurea and pyrimidine salicylic acid herbicides. Its herbicidal activity is activated by the metabolic processes in the plants.

离体条件下，化合物104-4 IC_(50)＞100 mg／L，而除草剂双草醚IC_(50)值为10~(-5)～10~(-4)mg／L，说明化合物104-4对离体ALS没有抑制作用；活体条件下，化合物104-4对ALS有一定的抑制作用，且随着处理时间的延长，酶活力降低，对ALS的抑制作用增加。

Methods Determination of DNJ by derivatization with 9-fluorenylmethyl chloroformate and followed by reversed-phase high-performance liquid chromatography and a fluorescence detector; the inhibitory curves on α-glucosidase at the enzymatic level and the activities to α-aglucosidase in vitro were observed in the everted sac experiment.

以氯甲酸药甲酯柱前衍生RP-HPLC荧光检测法对DNJ进行分析；在酶水平测定样品的α-糖苷酶抑制曲线，在离体水平用反转肠囊实验测定样品的α-糖苷酶抑制活性。

The biological tests indicate that most of the compounds synthesized have herbicidal activity in a certain degree and several compounds show certain bactericidal activity. Further anti-ALS enzyme test shows that two kinds of novel phosphonic ureas have good anti-ALS enzyme activity.

对合成的部分化合物进行了初步的生物活性测定，所设计的大部分化合物都显示出一定的除草活性，有的具有一定的杀菌活性；对两类膦酰脲化合物进行了离体ALS酶抑制试验，结果表明它们是很好的ALS酶抑制剂，同磺酰脲相当，有希望作为另一类ALS酶抑制剂的先导化合物。

Therefore, fluorescein isothiocyanate conjugated lectins, namely concanavalin agglutinin, wheat germ agglutinin and soybean agglutinin, were used to localize lectin receptors on plasma membrane of male and female gametes and other relevant sexual cells, as well as embryos of various developmental stages of Nicotiana tabacum and Torenia fournieri by cooled charge coupled device and Confocal Laser Scanning Microscope.

从而证实：在本实验中用的几种酶组合对凝集素受体的影响并不大，即由酶解及解剖得到的雌性细胞的荧光在分布形式及强度上没有明显的差异；对比原位酶解与离体酶解得到的雌性细胞的荧光，证实机械操作对荧光的分布形式及强度也没有明显的影响。

The toxical effects of nonylphenol ethoxylates and nonyiphenol were studied with EROD in liver of carp as biomarker under external condition. These two tested substances all had activation on EROD enzyme in liver of carp. Under definite range of concentrations, with the affecting rule of inhibiting at high concentration and inducing at low concentration.

在离体条件下，以鲤鱼肝脏EROD酶为生物标志物研究了壬基酚聚氧乙烯醚及壬基酚的毒性效应结果表明，在一定的浓度范围内，2种受试物对鲤鱼肝脏EROD酶均有激活作用受试物浓度对EROD酶的活性的影响规律为高浓度时抑制，低浓度时诱导。

The developer, trehalase amount, trehalose amount, reaction time and temperature, solvent were discussed. Validoxylamine test showed that the method of trehalase-inhibit in vitro is reliable for screening.

离体测试模型主要涉及溶剂的选择、显色剂选择、反应的温度和时间、海藻糖酶和海藻糖的用量等内容，所建立的离体测试模型用井岗霉素验证了该方法的可靠性。

This indicts that the biotic effects produced by treatment of high voltage electrostatic field are results of the seed system adapting adjusting to environment of high vollage electrostatic field.

但离体酶在静电场的作用下，其活性并未发生改变，说明种子经高压静电作用后产生的生物效应是种子对高压静电场环境自适应综合调节的结果。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷