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In the present experiment, the tender stems of Dracaena cambodiana were used as explants to establish a two-step tissue culture and regeneration system. The effects of types and concentrations of plant hormones on the induction of callus were studied. Based on these, the reactions of callus regeneration to different mutation-induced approaches (60Co irradiation, EMS and colchicines), the regeneration ability of the treated callus as well as the characters diversities (including the appearance traits and cytological observations) of the regeneration plants were investigated.

本试验以龙血树幼嫩茎段为材料,通过对龙血树幼嫩茎段两步培养法再生的研究,建立了龙血树幼嫩茎段离体再生体系,证明了愈伤组织的发生和激素种类与浓度的关系;并在此基础上研究了愈伤组织再生对不同人工诱变方法(60Co辐射、EMS、秋水仙素)的反应、被处理愈伤组织再生能力和再生植株性状多样性、以及再生植株不同生理角度的变异性,得到了2株变异株系。

RESULT: In vitro, TISE at the concentrations of 760 and 380 mgL-1 had significant antagonistic effect on the normal bronchus slice contraction caused by histamine as well as on the sensitized bronchus slice contraction caused by egg albumin, but had no effect on the contraction caused by acetylcholine. in vivo, TISE at doses of 25 and 18 mgkg-1 could significantly prolong the latent period of expiratory dyspnea, tic and falling down caused by egg albumin in sensitized guinea pigs.

结果:离体实验,TISE 760,380 mgL-1对组胺致正常豚鼠气管片的收缩具有对抗作用,对卵白蛋白所致的致敏豚鼠支气管平滑肌收缩有极显著的抑制作用(P<0.01),而对乙酰胆碱所致气管片收缩无明显影响;整体实验,TISE 25,18 mgkg-1可显著延长卵白蛋白所致的致敏豚鼠呼吸困难、抽搐和跌倒的潜伏期(P<0.01)。

In this section, we study the reactions of both potassium ferricyanide and hexaammineruthenium chloride at a 4-aminobenzoic acid modified glassy carbon electrode by SECM in different pH solutions.

应用扫描电化学显微镜对4-氨基苯甲酸修饰的玻碳电极进行了电化学表征,研究了酸度对电极表面修饰的4-氨基苯甲酸解离形态的影响,及不同的荷电中介体在此修饰电极表面的电化学行为。

ABSTRACT:OBJECTIVETo study the correlation between anatomic structure of the inferior alveolar nerve canal and dental implantation by measuring mandible innervation esemplarsMETHODSThe intramandibular course of mandibular canal and its dimendion were measured in 15 mandibles with teeth; the relationship between blood vessel and nerve was observed in 5 fresh samples in which the artery was filled; and then the data were analyzed by statisticsRESULTSThe mandibular canal was close to the lingual side and the inferior margin of the mandible, the mandibular canal and the 10mm underside of mandible were parallel in the region of the first and the second molars, it runned outboard to genal aperture when it was hereabout genal aperture, and the blood vessel lay above nerve in the mandibular canalCONCLUSIONIn implant operation performed according to normal anatomy, injury to inferior alveolar nerve may be avoided.

[目的]探讨下颌管的解剖结构与牙种植的关系[方法]选取15具牙列完整的成人离体下颌骨标本和5具经过动脉血管内灌注的新鲜标本,测量下颌骨在每个牙位截面上有关牙槽嵴及下颌管的相关数据,进行统计学分析[结果]牙槽嵴顶以及下方10mm宽度由前向后逐渐增宽;牙槽嵴由上向下逐渐增宽;下颌管位于下颌骨体内下方走行中偏舌侧,并近下颌骨下缘,在下颌第1,2磨牙区下颌管走行与下颌下缘成平行状,在近颏孔处转向外和向颊侧出颏孔;下颌管内血管位于下牙槽神经之上[结论]牙种植术中按正常解剖部位并且侧重颊侧骨板操作,可避免损伤下牙槽神经;如果术中下颌管内突然涌出大量新鲜血液,则提示若继续手术可能损伤下牙槽神经。

ABSTRACT:OBJECTIVETo study the correlation between anatomic structure of the inferior alveolar nerve canal and dental implantation by measuring mandible innervation esemplarsMETHODS The intramandibular course of mandibular canal and its dimendion were measured in 15 mandibles with teeth; the relationship between blood vessel and nerve was observed in 5 fresh samples in which the artery was filled; and then the data were analyzed by statisticsRESULTSThe mandibular canal was close to the lingual side and the inferior margin of the mandible, the mandibular canal and the 10mm underside of mandible were parallel in the region of the first and the second molars, it runned outboard to genal aperture when it was hereabout genal aperture, and the blood vessel lay above nerve in the mandibular canalCONCLUSIONIn implant operation performed according to normal anatomy, injury to inferior alveolar nerve may be avoided.

[目的]探讨下颌管的解剖结构与牙种植的关系[方法]选取15具牙列完整的成人离体下颌骨标本和5具经过动脉血管内灌注的新鲜标本,测量下颌骨在每个牙位截面上有关牙槽嵴及下颌管的相关数据,进行统计学分析[结果]牙槽嵴顶以及下方10mm宽度由前向后逐渐增宽;牙槽嵴由上向下逐渐增宽;下颌管位于下颌骨体内下方走行中偏舌侧,并近下颌骨下缘,在下颌第1,2磨牙区下颌管走行与下颌下缘成平行状,在近颏孔处转向外和向颊侧出颏孔;下颌管内血管位于下牙槽神经之上[结论]牙种植术中按正常解剖部位并且侧重颊侧骨板操作,可避免损伤下牙槽神经;如果术中下颌管内突然涌出大量新鲜血液,则提示若继续手术可能损伤下牙槽神经。

Cd〓 (1, 10, 100, 1000μM) stimulated directly GtH secretion from pituitary of common carp in vitro, with the highest GtH secretion at 100μM, GtH secretion stimulated with 1000μM Cd〓 was not higher than that stimulated with 100μM Cd〓.The antagonism between Ca〓 and Cd〓 in perfusion of the pituitary fragments was not obvious.After being preperfusion with 100μM Cd〓(with or without Ca〓), the GtH secretion of the pituitary fragment was irresponsive to 2-min pulse stmulation of 10μM sGnRH-A.

鲤鱼脑垂体碎片离体灌流孵育实验结果显示,Cd〓(1,10,100,1000μM)可直接刺激鲤鱼脑垂体分泌GtH,灌流液中Ca〓对Cd〓的拮抗作用不明显,100μM Cd〓刺激脑垂体分泌GtH达到高峰,Cd〓浓度增加到1000μM时,脑垂体分泌GtH并没有进一步增加,甚至有减少的趋势;100μM Cd〓预灌流脑垂体后,可使脑垂体对2-min 10μM sGnRH-A刺激的反应性消失;1.25mM的EDTA可消除100μM Cd〓刺激脑垂体分泌GtH的作用;TFP和Cd〓同时灌流脑垂体一段时间后,撤除TFP可使脑垂体分泌GtH显著增加,即出现"反跳"现象。

To probe into either the attractiveness to or avoidance from phylloxera of grape rootstocks and cultivars with different tolerance, as well as into the volatile components from their roots, the method of invitro root segment culture was used to investigate the selectivity of Phylloxera viticola Fitch to the roots of grape rootstocks '5BB' and '140Ru' and cultivar 'Kyoho'.

为了探讨不同抗性水平砧木及品种对根瘤蚜吸引及驱避效应以及鉴定挥发性物质组分,采用离体根段培养法测定葡萄根瘤蚜Phylloxera viticola Fitch对砧木5BB,140 Ru及栽培品种巨峰的选择性,发现根瘤蚜对栽培品种巨峰的根段具有很强的选择性,至接种后3d,67.13%的根瘤蚜优先选择巨峰;而对5BB和140 Ru的选择性却很差,分别有15.29%和17.58%的根瘤蚜选择5BB及140 Ru。

Effects of gamma irradiation on shoot regeneration from leaf and bud growth in vitro of strawberry 1 The leaf was very sensitive to the gamma irradiation, and 'Toyonoka' was more sensitive than 'Akihime'. The LD50 (50% decrease of generation) of strawberry leaf was between 10 and 20 Gy. 2 Strawberry leaves were inoculated in vitro at 8 d-15 d after gamma rays irradiation, and the shoot regeneration rate from of 'Toyonoka' increased at 10 Gy and almost no difference from 20 Gy to 40 Gy. The positive effect dose for 'Akihime'.was from 10 Gy to 30 Gy, however, 40 Gy decreased shoot regeneration rate.

2.60Co γ辐射对不同品种草莓离体叶片再生及芽生长的影响 1)叶片对辐射敏感,叶片再生的半致死剂量为10 Gy-20 Gy,'丰香'品种比'章姬'品种敏感; 2)辐射后8 d-15 d接种,'丰香'叶片的不定芽再生率在剂量10 Gy呈上升趋势,而其他剂量则几乎未见有变化,'章姬'在剂量10 Gy-30 Gy,叶片的不定芽再生率提高较大,40 Gy仍表现轻微下降; 3)适宜的辐射剂量对草莓试管苗增殖有促进作用。

The method makes model of small rat gastric ulcer, go alone with the home stomach of fill of colza leach fluid gives drug, observation test group and contrast the cankerous index of mucous membrane of group of small rat stomach, discuss its to advance a law to observe the home goes alone with charcoal end to protective action; of the stomach the influence that colza water steep moves to small intestine passes experiment leaving put oneself in another's position to observe its the action; of flowing to small rat small intestine flesh observes with heating up board law the home goes alone colza water.

方法制作小鼠胃溃疡模型,以家独行菜子水浸液灌胃给药,观察实验组和对照组小鼠胃黏膜的溃疡指数,探索其对胃的保护功能;以炭末推进法观察家独行菜子水浸液对小肠运动的影响并通过离体实验观察其对小鼠小肠平滑肌的功能;以热板法观察家独行菜子水浸液对实验动物的镇痛功能。

The anti-microbial activity of active materials against valsa mail,fusarium oxysporum,coniothyrium diplodieua and aspergillus nidulans were measured in vitro.the results showed that active materials extracted using acetone,aether and petroleum ether presented fungistasis to different extent on the four pathogenic fungi and had the best effect to coniothyrium diplodieua.active material extracted using ethanol presented certain anti-microbial effect to fusarium oxysporum.the results also showed there were anti-microbial active material in the leaves of cichorium intybus and founded a base on the research and application.

然后以提取液为供试药剂,对苹果腐烂病菌、棉花枯萎病菌、葡萄白腐病菌、构巢曲霉病菌4种植物病原真菌进行离体活性测定。结果表明:丙酮、乙醚、石油醚的提取液对4种病菌均有不同程度的抑制作用,其中对葡萄白腐病菌抑制效果最好;乙醇的提取液对枯萎病菌具有一定的抑制作用。表明菊苣叶中存在抑菌活性成分,补充了菊苣抑菌活性的研究内容,为该种植物抑菌活性成分的研究和应用奠定基础。

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