离体的

Orchidaceae has highly specialized morphological, structural, and physiological characteristics,it is an ideal material to study flower pigmentation and ovule initiation and development.

兰花离体培养开花系统的建立，可以用来探明兰花从营养生长向生殖生长的转变机制，是研究花的分化和发育的理想材料。

Orchidaceae has highly specialized morphological, structural, and physiologicalcharacteristics,it is an ideal material to study flower pigmentation and ovule initiation anddevelopment.

兰花离体培养开花系统的建立，可以用来探明兰花从营养生长向生殖生长的转变机制，是研究花的分化和发育的理想材料。

Most techniques in vivo monitoring the distribution, prolification and migration of transplanted cells. To objectively evaluate curative effect after cells transplantation, most techniques for the study of stem cell transplantation in animal models require histological analysis to determine the fate and migration of cells.

监测活体内移植入细胞的分布、增殖、迁移的方法虽然有多种，但传统方法都需要在离体状态下进行组织学切片分析和鉴定，无法准确地观察植入细胞的生长分化情况，从而难以对干细胞移植术的疗效作出客观评价。

Orange-spotted grouper is a benthic protogynous marine fish with sex inversion. It is one of the most important culturing fish in the southeast of Asia. As in other vertebrates, its growth is mainly controlled by growth hormone . Few data are available concerning the regulation of GH in grouper. In this paper, the regulation of growth hormone release from pituitary of orange-spotted grouper and its mRNA level was determined by radioimmunoassay and RNase protection assay.

中文题名斜带石斑鱼生长激素分泌及其基因表达调控的研究副题名外文题名 Regulation of growth hormone and its gene expression in orange-spotted grouper，Epinephelus coioides 论文作者冉雪琴导师林浩然学科专业水生生物学研究领域\研究方向学位级别博士学位授予单位中山大学学位授予日期2003 论文页码总数140页关键词生长激素基因表达石斑鱼馆藏号BSLW /2003 /Q959 /39 应用分子克隆技术，构建了斜带石斑鱼生长激素(Growth hormone，GH)基因重组质粒，在大肠杆菌细胞中获得高效表达，亲和层析纯化斜带石斑鱼GH融合蛋白做为抗原，免疫家兔制备特异性抗体，通过酶联免疫实验、放射免疫测定、离体脑垂体静态孵育和液相杂交／核糖核酸酶保护试验及化学发光检测技术，研究了斜带石斑鱼GH分泌及其mRNA表达的调控机制，对于斜带石斑鱼的生长、繁殖、人工养殖等具有重要意义。

Objective In order to reserve the nomally funictioning kidney with complex staghorn renal stone, remove the stone clearly and decrease the trauma, to evaluate the operation technique and clinical efficacy of hand-assisted laparoscopic nephrectomy,isolated pyelolithotomy autotransplantation of kidncy cure staghorn renal stone.

目的 为了既能保留功能良好的患有鹿角型结石的患肾，又能取净肾盂内结石和减轻创伤，探讨用手助腹腔镜取肾、离体肾切开取石、自体肾移植治疗肾盂鹿角型结石的手术方法和临床效果。

(1)The anatomic study of liver specimens reveals that retrohepatic segment of inferior vena cava and RHSIVC enclosed by liver parenchyma are comparatively long so it's safe and feasible to perform DIPS operation.

1。离体肝脏标本的应用解剖显示，肝后段下腔静脉及肝实质包绕的RHSIVC相对较长，实施DIPS是安全可行的；2。

The second was study on hepatocellular apoptosis during hepatic cold preservation-reperfusion. The third was investigation on effects of rewarm ischemia on the structure and function of reperfusing donor livers as well as its relevant mechanism.

实验分三个部分：离体供肝缺血-再灌注损伤模型的建立；供肝低温保存-再灌注期间肝细胞凋亡的研究；供肝复温缺血对再灌注后供肝功能的影响及相关机理。

Histological observation showed that normal structure was observed in all the groups before being harvested or after being preserved for 30 minutes. However, the more severe structure damage was observed in HPCs or hepatic sinusoidal cells with the more prolonged rewarm ischemia.

形态学观察发现，四组供肝于离体前及低温保存30分钟时的组织结构均无明显异常改变；而在复温缺血期间，各组供肝肝实质细胞及肝窦内皮细胞则表现有不同程度的结构损伤，且在程度上也与复温缺血时间呈明显的平行关系。

This experimental study, based on the ischemia-reperfusion injury model of the isolated rabbit liver, was to investigate hepatocellular apoptosis and its mechanism during cold preservation-reperfusion of donor liver and assess the structural and functional damages of the reperfusing donor liver subsequent to rewarm ischemia. In addition, the expression and effects of ICAM-1 mRNA were mainly observed during rewarm ischemia-reperfusion of donor livers. Our experiment included three parts: The first was establishment of the isolated liver ischemia-reperfusion injury model in rabbits.

本实验在建立离体供肝缺血-再灌注损伤模型的基础上，研究供肝低温保存-再灌注过程中肝细胞凋亡现象及其相关机制；探讨作为临床肝移植中供肝的另一缺血阶段—复温缺血对再灌注后供肝结构及功能的损伤作用，并着重观察供肝复温缺血-再灌注期间组织细胞内细胞间粘附分子-1 mRNA的表达规律及其作用。

The methods of low temperature stress and sugarcane inducing to make Free-proline accumulated in the isolated leaves of Saccharum Spontaneum L. and Sclerostachya were studied.

研究了诱导以甘蔗近缘野生种割手密、斑茅无性系的离体叶片等材料中的游离脯氨酸积累的低温处理、蔗糖诱导方法等。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新