离体的

Along with rapid development of bio-technology, artificial chromosome doubling in vitro is the efficiency approach of producing polyploidy plants. Advances on in vitro induction of autotetraploid in plants was reviewed.

随着生物技术的迅速发展，通过植物离体组织人工诱导多倍体已经成为获得多倍体植株的有效途径，本文就植物离体组织染色体加倍诱导同源四倍体的研究进展做一介绍。

To determine suitable surface sterilization methods, media and inoculation methods for grape stem and leaf explants and then establish testing methods for the effect of biocontrol Agrobacterium vitis E26 against grape crown gall in vitro.

［目的］筛选葡萄离体茎段和叶片的最适表面消毒条件、离体培养基和接种方法，建立生防菌株E26防治葡萄根癌病效果的离体检测方法。

During the 90 min experimental period, urine was collected in 10 min intervals and perfusate was collected at the midpoint of these intervals.

目的为建立发现和评价促尿酸排泄药物作用的离体模型，采用离体大鼠肾脏灌流技术，观察尿酸在离体肾脏中排泄特点和丙磺舒对尿酸排泄影响。

In the present experiment, the screening techniques for salt tolerance and mutants of strawberry leaf and anther callus in vitro were studied, and primary screening system for salt tolerance evaluation was established. It was studied the sensitivity to mutagen of different strawberry cultivars and different tissues in vitro.

本试验以草莓离体叶片及花药愈伤组织为试材进行了耐盐突变体的诱变及筛选研究，初步建立了草莓耐盐突变体诱变筛选的技术体系，研究了草莓品种以及离体组织对诱变剂的敏感性，并且对不同诱变处理再生植株的耐盐性差异进行了比较。

The paper summarizes studies of in vitro inducement of haploids in cucurbitaceae, including anther culture, in vitro gynogenesis culture and induction of haploid obtained by irradiated pollen. The factors affectting in vitro inducement such as material genetype, medium, pretreatment, megaspores development stage and others had significant effects on the embryoids inductivities.

重点对葫芦科植物离体诱导单倍体的研究进行综述，包括花药培养、离体雌核培养、辐射花粉诱导单倍体培养等在育种中的应用，以及供体基因型、培养基、预处理方式、胚囊发育时期等因素对离体诱导再生频率的影响。

In this research, we have studied the effects of a series of factors (genotypes, basicmedia, plant growth regulators, explants, inoculation methods, leaf ages and darkperiod) in the inducing adventitious shoot of strawberry(Fragaria ananassa Duchcv.

本试验以草莓品种&丰香&和&章姬&为试材，研究了草莓离体再生过程中基因型、基本培养基、植物生长调节剂配比、不同外植体、接种方式、苗龄和暗处理对草莓离体再生不定芽的影响，获得了草莓高效的离体再生体系。

Segments produced clusters of somatic embryos from embryogenic calli or directly from root apical meristems , and that also can convert into bud directly in the different tissue culture conditions. Modified media B5 with 1.0 mg.L-1 2,4 - D and 3.0mg.L~1 6-BA is suit to induce calli, and 0.5 mg.L-1 NAA is fit for somatic embryos inducement from calli. 6-BA encreased direct somatic embryogenesis on root tip distinctly,but NAA is retard it. NAA also prolong the time of root-tip convert into bud directly.

在改良B_5(微量元素为原来的1／3)中加入1.0mg.L~(-1)2，4—D和3.0mg.L~(-1)6-BA对离体根尖的愈伤组织诱导较为合适，0.5mg.L~(-1)NAA有利于从愈伤组织上产生体细胞胚；6-BA明显促进离体根尖直接产生类原球茎，而NAA则有阻碍作用；同时NAA的存在也使离体根尖直接成芽的时间延长。

Furthermore there are more apoptosis cells in inner root sheath than in outer root sheath. Conclusion When we culture human hair follicle in vitro, Williams E serum-free medium is a suitable choice.This model is a fine model also, which is used for screening drug that may accelerate or inhibit the hair growth.Cryo-section can simplify procedure of paraffin section in histology detection of culture human hair follicle in vitro.We can observe the ultrastructural change of human hair follicle in vitro by transmission electron microscope,which can assist to observe apoptosis cells. The eyepiece micrometer is a simple tool for measuring length of hair follicle in vitro.

在离体培养人头皮毛囊时，Williams E无血清培养基是合适的选择，该模型也是筛选促进或抑制毛发生长药物的良好模型；离体培养毛囊组织学检测用冰冻切片可以简化步骤；透射电镜可以用来观察离体培养的毛囊的形态和超微结构的变化，也可以用来协助观察细胞凋亡的状况。

The contact angletoward water of the epoxy resin modified by the polysiloxane ionomers HQAS-PDMS was related with the surface energy of the substrate, ion group content and thelevel of the ionomers.

用硅氧烷离聚体HQAS-PDMS改性的环氧树脂固化物对水的接触角与成型基质的表面能、硅氧烷离聚体的离子基团含量、硅氧烷离聚体的加入量有关。

By removed ovary culture after positive and negative hybridization, produced interspecific hybridization, and have improved the hybrid seed through removed ovary culture and induced to shoot by culture medium MS, and produced hybrid F1, the plant of Brassica junce×B.pekinesis, the advantage characteristic of hybrid plant is similar to female nature;By cytology authenticating, chromosome number of F1 young flower bud cell (2n=28), it is the sum of monoploid chromosome number of female parent Brassica junce(2n=36) and male parent B.pekinesis(2n=20); The hybridization to Brassica junce×B.pekinesis, and embryonic bud come from removed ovary culture, and it found another form of plant embryo saving.

以芸薹属植物芥菜型油菜和白菜为研究对象，对其进行常规正反杂交后子房的离体培养，通过离体子房培养获得的杂种种子经MS培养基诱导成苗，获得了芥菜型油菜与白菜的杂种F1代植株，表现出杂种优势；通过细胞学的方法，鉴定了杂种F1幼蕾体的细胞染色体数为2n=28，为母本芥菜型油菜（2n=36）和父本白菜（2n=20）的单倍体染色体数之和；芥菜型油菜与白菜常规杂交后，经离体子房培养获得了&胚性芽&，确立了植物胚挽救的另一种形式。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。