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The effects of anodyne and anticonvulsant of GLJ is proved by experiment, further research of its mechanism is under processing.

本论文采用在体和离体动物实验对高良姜超临界萃取物抑制肠平滑肌痉挛的作用进行了探讨,以期研究其作用机制,为研究和开发新药提供实验依据。

Objective To study the effects of Jin'an tablets on contractile activities of uterine smooth muscles in rats both in vivo and in vitro.

目的 观察经安片对大鼠离体和在体子宫平滑肌运动的影响。

Recovery of hematopoiesis depends on the ability of proliferation and self-renewal of surviving cell after radiation injury.

由于缺乏射线诱导在体造血细胞凋亡的研究,尚不能确定离体实验的结果对于在体损伤的应用价值。

W7 was added when the cells entered anaphase, we found that although some cells had elongated, the distribution patternof CaM at central spindle was normal, and it seemed that a cleavage furrow would possibly form soon, however, these cells stopped cytokinesis at this stage, no furrow ingression followed.

本实验中我们还观察到在胞质分裂后期,CaM与Y一微管蛋白共分布于中体两端。W7处理同步化在胞质分裂后期的细胞,不但使中体解聚发生延迟,而且抑制了Y一微管蛋白与中体的解离。

In order to understand the biology of apple ring spot fungi further and solve the problem of less conidiophores generated during their inducing culture in vitro, it was studied that the effect of culture medium pH, incubation temperature, light sources, and light regiment on growth of mycelia and generation of conidiophores, and the removing of mycelia on generation of conidiophores.

为进一步了解苹果轮纹病菌的生物学特性,解决其在离体培养条件下产生分生孢子很少的问题,研究了培养基pH值、培养温度、光源和光照条件对菌丝体生长和分生孢子发生的影响以及刮除菌丝体对分生孢子发生的影响。

Radiolabeled pharmacological binding assay showed: 1 BmK AS bound specifically to a single class of non-interacting binding sites on both rat brain and cockroach nerve cord synaptosomes with high affinity and low low capacity. The association of BmK AS with both synaptosomes was quick to reach equilibrium, but the dissociation seemed to be only partial. The specific binding of BmK AS was independent of membrane potential, and couldn't be modified by veratridine. Moreover, the binding of BmK AS could be inhibited by native BmK AS, BmK AS1, BmK IT2 and BmK IT on both synaptosomes, but not or partially inhibited by BmK Ⅰ and suggesting BmK AS type neurotoxins might bind to a distinct receptor site on mammal and insect sodium channels with a similar manner from β-scorpion toxins.

二。蝎毒素多肽配体与哺乳动物和昆虫靶离子通道受体的药理结合特性及其靶结合位点的研究:经放射性标记结合和竞争抑制实验表明:1)BmK AS在大鼠脑和蟑螂神经索突触体上有单一非协同性高亲和力低结合容量结合位点,能快速结合并达到平衡,但仅能部分解离,其结合不依赖于膜电位,也不受藜芦碱调节,天然的BmK AS,BmK AS-1,BmK IT2和BmK IT可抑制〓AS与两种突触体的特异结合,而BmK Ⅰ无或仅有部分抑制效应。

The results show that the suitable material for treatment is the bulb basal plate tissue with a thin part of scales after having been inoculated for 7 days,and regeneration dose is 50~60Gy,the optimum treatment dose for breading is 15~20Gy.In the small bulbs treated with 15Gy and 20 Gy,two mutants were selected,one is shorter with more leaves,the other expands faster.

结果表明,接种7D的外植体更适合作为水仙离体诱变的材料,其不分化剂量为50~60GY,半剂量为15~20GY,在10和15GY处理中,发现了两种小鳞茎突变类型,一种为叶片数增多的矮化突变体,一种为试管小球茎膨大速度加快,所以适宜辐射诱变的剂量为10~20GY。

The results show that die suitable material for treatment is the bulb basal plate tissue with a thin part of scales after having been inoculated for 7 days, and regeneration dose is 50~60Gy, the optimum treatment dose for heading is 15~20Gy. In the small bulls tinted with l5Gy and 20Gy, two mutants were selected, one is shorter with more leaves, the other expands faster.

结果表明,接种7d的外植体更适合作为水仙离体诱变的材料,其不分化剂量为50~60Gy,半剂量为15~20Gy,在10和15Gy处理中,发现了两种小鳞茎突变类型,一种为叶片数增多的矮化突变体,一种为试管小球茎膨大速度加快,所以适宜辐射诱变的剂量为10~20Gy。

The callus induction of licorices was easy. And the MS+1.0 mg/L NAA+1.0 mg/L 2, 4-D +1.0 mg/L 6-BA was the best medium to cotyledons, hypocotyls and radicles. And all the induction rates were 100%. 4. In the study of callus regeneration, only obtain the regeneration plants of hypocotyls callus,and its frequency was 4.57%.

甘草离体再生中愈伤组织的诱导较为容易,三种外植体子叶、下胚轴和胚根均以MS+1.0 mg/L NAA+1.0 mg/L 2,4-D+1.0 mg/L 6-BA为愈伤组织诱导的最佳培养基,在此培养基上,3种外植体愈伤组织诱导率均达到100%。

The results showed that DpCPV-W could amplify in vitro in Sf21 cell and form normal polyhedra.

研究了文山松毛虫质型多角体病毒在Sf2 1细胞中的离体增殖行为,并进行了空斑试验,结果显示DpCPV W毒株能够在Sf2 1细胞中增殖,也能在Sf2 1细胞上形成空斑,并能产生形态正常的病毒多角体。

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