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福尔马林

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METHODES:The antinociceptive effect of CPF was observed at the absence and presence of α-fluoromethylhistidine, a specific inhibitor of histidine decarboxylase, in three hyperalgesic models of mice (hot plate test, writhing test and formalin test).

用3种不同的小鼠痛觉模型(热板法、扭体法和福尔马林实验)观察CPF的镇痛作用。

In the second test, another group of bigger turbot fries were vaccinated with formalin-killed vaccine of V.anguillarum via intraperineal injection.

第二次试验中,鱼苗较大,水温12-16℃,以福尔马林灭活的鳗弧菌W-1疫苗注射免疫大菱鲆鱼苗,于8周后同法进行二次免疫。

Morphine tolerance increased the expression of formalin-induced NADPH-d positive neurons in the superficial laminae of spinal dorsal horn.

吗啡耐受增加福尔马林致痛大鼠脊髓背角浅层NADPH-d阳性神经元、纤维及终末的表达。

Methods: Micromembrane filtration,ethyl acetate-formalin separation,giemsa stain.

微孔滤膜过滤、乙酸乙酯福尔马林分离、G iem sa染色、油镜及相差显微镜观察。

If the fish have not improved within 5-7 days, perform a 50% water change and treat with formalin or an organophosphate insecticide used for removing the monogenetic flukes.

如果鱼没有改善, 5-7天内,执行50 %的水分变化和治疗与福尔马林或一有机磷杀虫剂用于消除

It is therefore concluded that FA can directly activate a subpopulation of primary nociceptor cells and the FA-induced AP discharges are likely to contribute mainly to phase 1, but not phase 2 of the formalin-induced nociception.

以上结果提示,甲醛溶液选择性地激活一个亚群的初级传入伤害性感受器细胞,甲醛激活的动作电位的发放主要参与福尔马林诱发的第一相伤害性感受的产生。

The formalin test is a commonly used animal model of acute and tonic pain. However, the molecular targets of formaldehyde (FA, the main ingredient of the formalin solution) on primary nociceptor cells remain controversial.

福尔马林致痛模型是化学伤害性刺激引起急性和持续性痛常用的动物模型。

A formalin-inactivated RSV vaccine was used to immunize children and elicited nonprotective, pathogenic antibody.

使用福尔马林灭活病毒疫苗给婴幼儿进行免疫接种,无法产生保护性抗体,而导致了致病性抗体的生成。

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度,然后每个角膜被分成两半,共48个半片角膜,再分成4组,每组12个半片。12个半片用茜素红-台盼蓝染色染色行角膜内皮细胞计数;12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变;12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性;12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜,用茜素红-台盼蓝染色染色行角膜内皮细胞计数;用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变;用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性;实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变;并于正常对照组角膜比较。

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