福尔马林

And try to find out the effect of TSA on the proliferation and differentiation of rat tracheal stem cell. Methods 1.Preparation of 5-Fu Model and TSA treatment Two weeks" Wistar rats provided by the animal center of the China Medical University. The animals were sacrificed by 10% Chloral Hydrate 0.4ml/100g. Then the tracheas were excised aseptically. Then they were douched with PBS and cultured in 1:1 mix of Dulbecco"s modified Eagle"s medium and Ham"s F-12 medium (DMEM/F12) containing 120 mg/ml 5-Fu and 10% fetal bovine serum for 12h at 37°C. The tissues were then refreshed by culturing in DMEM/F12 containing 10% FBS and 200nM TSA.

1、制备气管损伤模型及剥离上皮取约200克左右的Wistar大鼠，雌雄不限(中国医科大学实验动物中心提供)，腹腔注射10%水合氯醛0.4ml/100g，无菌条件下取出气管，无菌PBS反复冲洗，洗净血液和粘液，置于DMEM/F12培养液中(含10%胎牛血清)，于培养液中加入终浓度为100mg/ml的5-Fu，37℃，5%CO2孵育12小时，弃去上述培养液，换成新鲜DMEM/F12液(含10%胎牛血清)，同时加入终浓度为200nM的TSA继续培养，于换液后3、6、12、24、48小时分别10%中性福尔马林固定，石蜡包埋，制成4μm厚的组织切片。

RESULTS: Intrathecally administered WIN 55,212-2, bupivacaine,or their combination produced a dose-dependent decrease in thenumber of flinches during Phase 1 and 2 of the formalin test.

结果：鞘内注射 WIN 55，212-2、布比卡因或二者混合液可在福尔马林试验的1期和2期产生剂量依赖的退缩数量减少。

Finally, local effects of CPZ were confirmed in the formalin test and it was shown that the formalin-induced paw flinches were strongly suppressed by CPZ in phase 1 but with phase 2 being significantly suppressed only during 25-55 min.

利用行为学技术方法证明足底注射CPZ可以明显抑制福尔马林溶液所致的第一相全程和第二相内仅第25~55 min的自发缩足反射。

Objective: After rat be rended to supply Ginkgo biloba, through observing expression of c-fos in brain of rat being induced to pain by formalin. Method: Fiffy female rats were randomly divided into 5 groups, each group contaiued 10 rats.

目的：通过给予福尔马林致痛大鼠银杏叶片的混悬液制剂后，观察大鼠脑区c-fos的变化，来探讨银杏叶对伤害性刺激所致脑内变化的作用的机理。

In the 56 , 22 were in sinus ventriculi, 6 in corpus ventriculi and 28 in fundus and cardia ventriculi.Tumoer size that was smaller than 5cm were 34 cases,while that was bigger than 5cm were 22 cases.

所有标本常规经10％福尔马林固定，石蜡包埋，定向切片；分别采用NF-κBp65鼠单克隆抗体和uPA兔多克隆抗体，以S-P法进行免疫组织化学染色，观察其在各组织标本中的表达。

There are many discrepancies in earthworm sampling methods ( hand-sorting, electrical octet, formaldehyde, mustard and allyl isothiocyanate, abbreviated AITC ). All of the methods have some disadvantages.

常用的蚯蚓采样方法如手挖、电击法、福尔马林法、芥末法、异硫氰酸丙烯酯法存在很多差异，各方法都存在一定缺点。

Methods: We studied thirteen hips of ten formalin-fixed adult cadavers.

我们研究了10具福尔马林固定的成人尸体的13个髋关节。

Experimental materials 15 fresh human cadaver livers, contrast media (Ultravist 300), acrylic resin, silicone, polyurethane foam, colourant, P45;...

实验材料：新鲜人尸体离体肝脏15个，对比剂(优维显300)，铸型剂JF，硅胶，发泡剂，油画染料及纺织染料、水粉染料若干，20％福尔马林溶液，15％盐酸溶液，聚酯共聚体P45。

The serotype of 66 strains of Vibrio harveyi isolated from large yellow croaker(Pseudodciaena crocea Richardson) infected by vibriosis was tested in this study. The juvenile large yellow croakers, which cultured in the cage in Xiangshan Bay , were vaccinated with formalin killed V.harveyi of the virulent strains by immerse route , The results were summarized as follow.

本文研究了从患病大黄鱼体内分离66株哈维氏弧菌(Vibrio.harveyi)的血清学性状，并选取了其中的强毒菌株制备成福尔马林灭活的V.harveyi菌苗，对象山港网箱养殖大黄鱼进行了浸泡免疫试验，探讨了V.harveyi的浸泡免疫保护效果，得到了如下研究结果。

Most laboratories use neutral-buffered formalin (10%) for tissue fixation which introduces cross-links, whereas coagulative fixatives are less popular. Problems with formalin fixation comprise delay of fixation and variations in the duration of the fixation mainly.

绝大多数的实验室使用中性福尔马林固定、固定引起的交联、延迟固定和固定时间的导致的影响等这些问题得到解决，提出组织固定时间在手术切除后的30分钟内进行固定，同时避免过度固定，时间超过24-48小时。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。