神经鞘的

To perform large-scale multiplex analysis of antibody responses to lipids in multiple sclerosis, we developed microarrays composed of lipids present in the myelin sheath, including ganglioside, sulfatide, cerebroside, sphingomyelin and total brain lipid fractions.

我们采用微列阵技术对多发性硬化脂质反应性抗体进行大规模多重分析，微列阵组成包括髓鞘中的脂质，其中包括神经节苷脂、脑硫脂、脑苷脂、鞘磷脂、总的脑脂质。

Large numbers of experiments internal and external had confirmed both direct current and square pulse of distal end cathodal could improve peripheral nerve regeneration, because it could speed up transmigration, creeper, growth and development of cell Schwann, derivate growth of nerve fiber; it also increased blood capillary of epineurium, brood blood vessel, amendment ischemic of impaired nerve, it still could accelerate Wallerian

国内外大量的基础实验及临床研究都证实远端负极电流的直流电场及脉冲电场等均有促进周围神经再生的作用，认为其可加速Schwann细胞的游走、爬行及生长发育，诱导神经纤维的生长；电刺激还能促使神经外膜毛细血管数量增多，扩张血管，改善神经损伤段缺血状态，并加速Wallerian变性及崩解组织的清除，为Schwann细胞发育和髓鞘化过程提供充足的能量与物质，同时为神经轴突的再生提供良好的内环境；在神经断端出现瘢痕或神经瘤时，电刺激可增加神经纤维穿越瘢痕的能力。

Methods 14 Sprague-Dawley rats with T9 spinal cord hemisection were divided into 2 groups: control group and olfactory ensheathing cells transplantation group .

第二部分：嗅鞘细胞移植促进大鼠脊髓损伤后功能恢复目的探讨嗅鞘细胞移植对脊髓损伤的功能恢复和促进神经再生作用。

RESULTS: Chitin tubes containing NGF successfully induced the nerve regeneration, regularly arranged myelinated and unmyelinated axons could be observed across the 8 mm gaps, and the myelin sheath was thick with clear lamellar structure at 8 weeks after operation, The regenerated nerve fibers increased and were more mature at 16 weeks after operation.

结果 实验侧术后8周，再生神经中有髓和无髓神经纤维排列整齐，有髓神经的髓鞘厚，板层结构清晰；术后16周，再生神经中有髓和无髓神经纤维数量增加，形态接近正常神经。

METHODS: We performed neurological examination, neurophysiological, neuroimaging, and neuropathological studies on sural nerve biopsy in 10 hypomyelination and congenital cataract patients from 5 unrelated families.

我们进行神经系统检查、神经生理学、神经影像学和腓肠神经活检的神经病理学研究，这些是对来自于5个不相关家庭的10例先天性白内障和神经髓鞘形成不良患者进行的检查。

While the tissue spaces surrounding a few blood vessels wasAl and Fg positive,no Al or Fg positive cells were observed.In antemortem injurygroup,diffuse subarachnoid hemorrhage,cerebral edema,swelling or pyknotic neu-rons could be observed.The axons showed irregular swelling and disconnection at1～3h,marked swelling and disconnection at 6h,and retraction ball at 15h whichwas more remarkable at 24h after injury.The space between myelin sheaths andaxons was increased at 3～6h after injury.Tortuous and wavelike myelin sheathswhich adhered on axons incompletely,or even peeled off could be found from 15hto 24h after injury.Perinuclear lysis of Nissl bodies began at 24h after injury.Thenumber of GFAP positive cells in cerebrum and brain-stem increased significantlyfollowed by decrease,and then increased again,but the time courses of the changesin different areas of brain were not same.Al and Fg positive neural cells,mainlysurrounded blood vessels,with diffuse or peripherally distributed positive matter incytoplasm could be observed at 0.5h after injury.The number of Al or Fg positivecells and the intensity of immunoreaction increased with the time of injury.The areaof SYN positivity in medulla oblongata and pons decreased notably 3～6h afterinjury,then return to normal levels and continued to 24h after injury.

生前损伤组，可见广泛蛛网膜下腔出血，脑组织水肿，神经细胞肿胀，晚期神经元固缩；伤后1～3h见部分神经轴突不规则增粗、断裂，伤后6h断端膨大，伤后15h可见收缩球，至伤后24h更为明显；伤后3～6h可见部分神经髓鞘与轴突之间的间隙增宽，伤后15h髓鞘明显曲折，不完全附着在轴突两侧，甚至剥脱，持续到伤后24h；核周尼氏体减少在伤后24h才开始出现；同一部位的GFAP阳性细胞数目随损伤时间发生改变，先增多（最早在伤后0.5h），达到高峰后减少，其后又有增多趋势，但不同部位的GFAP阳性细胞数目增减的时间过程不尽相同，同时，大脑中的GFAP阳性细胞数目也有改变；伤后0.5h，可在脑干组织中见到Al和Fg阳性神经细胞，主要位于血管周围，阳性物在胞浆中呈弥散性分布，但部分细胞的阳性物仅分布于靠近胞膜的胞浆中而呈环状，随损伤时间延长，阳性细胞数目增多，反应强度增加；伤后3～6h，延髓及桥脑中的SYN阳性物面积减少，其后恢复到正常水平，并持续到伤后24h。

The adipose derived stem cells of inbreds train of rat wereinduced to differentiate into schwann-like cells and the cells were injectedinto the acellular nerve allograff.The proliferation or adhersion of these cells on the graft were observed by inverted microscope or ScanningElectronic Microscope. Activity of cells were detected using MTT. 10mmnerve gap of inbreds train of rats in two group were bridged by tissue-engineered peripheral nerve or autogenous nerve, the effect wereappraised by naked eye, recovery rate of sciatic function index,nerve-electrophysiological, histology, Transmission ElectronMicroscopy and quantitative analysis of recovery rate of myelinated fiberpopulations,diameter of myelinated fiber and thickness of myelin sheat.

按照前述方法培养并诱导近交系大鼠脂肪干细胞向类Sehwann分化，将诱导分化的脂肪干细胞悬液注入已制备的去细胞同种异体神经支架管中，倒置显微镜观察细胞生长情况；扫描电镜下观察细胞在材料上附着情况；四唑盐比色试验测定细胞活性；取两组近交系大鼠，分别用组织工程化神经和自体神经桥接10mm神经缺损，通过大体观察、坐骨神经功能指数恢复率的测定、神经电生理的测定、组织学光镜观察、透射电镜观察、再生有髓神经纤维计数恢复率、神经纤维直径恢复率、髓鞘厚度恢复率的测定等指标评价实验效果。

Results: Dynamic observation with histopathology and immunohistochemical staining showed that, after the nerve root nerve root compression, the myelinated nerve fibers decreased and there was ultrastructure changes of the nerve fibers, such as Schwann cell cytoplasm swelling and edema, Wallerian degeneration, and demyelinated degeneration of axons.

结果：通过对压迫神经根及神经根节大体观，组织病理学和免疫组织化学的光学显微镜的动态观察，可见神经根压迫后其有随神经纤维数目减少和超微结构的改变，如神经模细胞胞质肿胀和细胞水肿、华勒变性、神经轴突脱髓鞘变等。

All the indexes were tested in 2 -. 4 .. 6 weeks of operative intervals respectively. Results:(1) The general observation in the experimental group ,such as spirit, hair, body weight and activity ,was superior to that in the control group.(2) The recovery rate of sciatic nerve functional index (SF1) was statistically significantly higher in the experimental group than in the control group .(3) electrophysciological indexes: m the experimental group ,the tardy rate of induced motorius action potential ,recovery rates of the amplitude of compound muscle action potential and twitch tension and tetanic tension of triceps surae muscle ,were significantly higher than in the control group.(4) Compared with the control group ,the triceps surae muscle wet weight was significantly greater in the experimental group.(5) histomorphological indexes: the count of myelinated nerve fibers ,diameter of regenerated axon, thickness of nerve myelin sheath and area of capillary were much more in the experimental group than in the control group.(6) observation of ultrastructure with the transmission election microscope: more abundant organell a and maturer myelin sheath lay in the injured peripheral nerve of the experimental group, while not lay in the control group.

分别在木后第2、4、6周时进行各项指标的检测，结果发现：（1）实验组SD大鼠在整个实验过程中，其精神、毛发、体重，运动灵活性等一般情况优于对照组；（2）实验组的坐骨神经功能指数恢复率显著优于对照组，P＜0.01；（3）神经电生理指标：实验组运动神经诱发电位潜伏期的延迟率显著优于对照组，P＜0.05；复合肌肉动作电位振幅的恢复率显著高于对照组，两组比较第2周时P＜0.01，第4、6周时，P＜0.05；小腿三头肌单收缩力和强直收缩力的恢复率实验组显著高于对照组，P＜0.01；（4）小腿三头肌湿重的恢复率，第2、4周时实验组高于对照组，差异非常显著，P＜0.01；第6周时，差异仍显著，P＜0.05；（5）组织形态学指标：有髓种经轴突计数、再生轴突直径的恢复率，毛细血管面积，实验组均显著大于对照组，P＜0.01、P＜0.05、P＜0.001；实验组髓鞘厚度的恢复率也显著高于对照组，第2周时P＜0.01，第4、6周时，P＜0.05；（6）透射电镜超微结构观察：实验组再生轴突中细胞器丰富，髓鞘结构成熟，神经再生情况优于对照纽。

Result: Beneficial effects on nerve conduction speed , fiber diameter, fiber amount and thickness of medullary sheath were also observed in the permeable PLA NGCs.

实验结果：术后三个月聚乳酸导管/神经生长因子微囊组在神经传导速度及形态学中的再生纤维直径、纤维数、纤维密度、髓鞘厚度等均优于聚乳酸导管直接吻合组及聚乳酸导管内注入神经生长因子组；和自体神经移植组的结果基本相似。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。