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The results show that firstly, ApoE and LDL receptors can be transported bidirectionally (i.e. retro-and anterogr-adely) in the axoplasm along axons. Secondly, ApoE derived from regenerating fillers can be transported re t rogradely to the cell bodies.

本文用免疫细胞化学法研究正常和再生的大白鼠外周神经中脱辅基脂蛋白E和低密度脂蛋白受体的运输特性,发现在正常的外周神经中,ApoE及LDL受体在轴浆中均可沿轴突正行和逆行运输;再生的神经可产生ApoE,ApoE可逆行向细胞体转运。

14Th week after injury, the reorganization of labelled neurons' distribution was found in the autograft group, but only one case in the NTN/PGLA group. Conclusion Local application of PGLA tube with neurturin can recover the axoplasm flow of regenerated nerve fibers, its long-term effect can match that of the nerve autograft, and collateral axonal branching occurred less.

应用NTN/PGLA导管的修复能够和自体神经移植修复一样恢复神经缺损的连续性和逆行轴浆运输功能,后期效果相当;应用NTN/PGLA导管修复后神经元分布异位明显少于自体神经移植修复,提示较少发生轴突误向再生,减少面肌联带运动的发生。

4DRG was co-culture with sciatic nerve segment in 10鸖 DMEM;the axons were longer and surround the sciatic nerve segment which was regard as anew evidence for chemotropism.

结果:(1)在无血清条件下单独培养的DRG,背根神经节的轴突数目众多,外形纤细弯曲,不成束,并且施万细胞和成纤维细胞稀少,所以可以排除两者对轴突生长的影响,为观察来源于变性坐骨神经段的可溶性因子对轴突生长的作用提供了有利条件;(2)在无血清条件下DRG和变性坐骨神经段联合培养,①先单独培养DRG,4天后待神经元轴突长出,再与坐骨神经段联合培养,观察到神经元的轴突数目减少,外形挺直,部分轴突之间相互粘附成束;②变性坐骨神经段和DRG同时联合培养,神经元的轴突数目明显减少,外形粗壮,轴突之间相互粘附成束;(3)有血清条件下单独培养DRG,轴突数目较多,外形挺直,长短不一,部分神经元的轴突之间相互粘附,施万细胞和成纤维细胞数目众多,观察到的轴突生长情况受到施万细胞和成纤维细胞的直接或者间接的影响。

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neurulation; neuroblasts; neural stem cells; neuroprogenitor cells; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; neurogenesis; development; developmental stages

神经外胚层;神经脊;神经板;底板;神经管;轴索;神经胚形成;成神经细胞;神经干细胞;神经母细胞;干细胞;分化;个体发生;形态发生;组织发生;器官发生;突触发生;gangliogenesis;胚胎发生;axonogenesis;retinogenesis;gliogenesis;神经胶质祖细胞;少突神经胶质细胞祖细胞;视网膜祖细胞;神经生长因子;神经营养因子;营养因子;生长因素;神经管故障;无脑;脊柱裂;成神经细胞瘤细胞;神经发生;发展;发展进程

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neurulation; neuroblasts; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层;神经脊;神经板;底板;神经管;轴索;神经胚形成;成神经细胞;干细胞;分化;个体发生;形态发生;组织发生;器官发生;突触发生;gangliogenesis;胚胎发生;axonogenesis;retinogenesis;gliogenesis;神经胶质祖细胞;少突神经胶质细胞祖细胞;视网膜祖细胞;神经生长因子;神经营养因子;营养因子;生长因素;神经管故障;无脑;脊柱裂;成神经细胞瘤细胞;细胞迁移;神经发生;发展;发展进程首页上一页下一页末页共有 22 条记录,本页从第 11 到第 20 条。

The pituitary gland consists of neurohypophysis and adenohypophysis. Two types of pituicytes and three types of neurosecretory fibers (A1, A2 and B) have been recognized in neurohypophysis. The neurosecretory terminals contain different diameters and shape of secretory granules.

脑垂体由神经垂体和腺垂体2部分组成神经垂体组织中存在2种类型的脑垂体细胞,并可以区分A1型、A2型和B型3种类型的神经分泌纤维,其终端轴突中具有不同直径和形态的分泌颗粒;B型纤维轴突中含有许多透明小囊泡。

RESULTS: The mild traction group showed angulus anterior individual neuron slightly engorged, neuron and nerve fiber morphous was normal essentially; individual substantia alba demyelinates in fragmentis; medullary sheath and auxiliary fibers of nerve root were hydropsia slightly without conspicuous myelinolysis. The moderate traction group demonstrated myelinolysis change in the spinal cord, and neurofibras lined up chaos, auxiliary fibers disaggregated, neuron dropsy, tigroid body disappeared with karyopycnosis and anachromasis in neurons; nerve root showed myelinolysis. The severe traction group displayed a great demyelination region; anterior motor neurons with karyopycnosis and anachromasis; myelinated nerve fiber of nerve root shrinked, decreased in number, with severe demyelination changes.

结果:轻度牵拉组牵拉侧前角个别神经元稍肿胀,神经元及神经纤维形态基本上正常,个别白质小片状脱髓鞘样改变,神经根髓鞘和轴索轻度水肿,脱髓鞘不明显;中度牵拉组白质有脱髓鞘改变,神经纤维排列紊乱,轴索崩解、断裂;灰质神经元水肿明显加重,前角运动神经原细胞尼氏体消失,核固缩、深染,神经根髓鞘和轴索水肿,局部轻度脱髓鞘改变;重度牵拉组牵拉侧白质为不规则的大片脱髓鞘区,前角运动神经元胞体固缩、变形、核深染,神经根有髓神经纤维萎缩、数目减少,重度脱髓鞘改变。

Results: 1. The depression patients in neuroendocrine abnormity make up 59.4% of the total. 2. Correlation of clinical features and neuroendocrine diversity of depression patients.① Agitate symptom often existed in HPA axle abnormal group.② The weight diversity was not obvious suffered HPT axle abnormal depression frequently, Sleep disorder and anxiety often exist in HPT axle abnormal group.③ Elder women suffered HPG axle abnormal depression frequently, The symptom of somatic often exist in HPG axle abnormal group.④ Self-reproach and delay symptom often existed in the HPA axle and HPT axle abnormal group.⑤ The adolescent fuffered the normal neuroendocrine group, Patients of the normal neuroendocrine group were often combined with anhedonia.

结果显示:⒈抑郁症患者神经内分泌异常率达到 59.4%;⒉抑郁症患者临床特征与不同神经内分泌轴具有相关性以下几点值得注意;① HPA 轴异常组抑郁症患者多伴有激越症状;② HPT 轴异常组抑郁症患者体重改变不明显,多伴有睡眠障碍、迟纯症状和焦虑症状,常出现自杀观念;③ HPG 轴异常组抑郁症患者多发于年龄偏大的女性,躯体症状多明显;④ HPA 轴和 HPT 轴均异常组抑郁症患者多伴有体重减轻和明显自责和迟滞症状;⑤正常组抑郁症患者多发于青少年,快感缺失症状明显。

Neuroectoderm; neural crest; neural plate; floor plate; neural tube; neuraxis; neurulation; neuroblasts; stem cells; differentiation; ontogeny; morphogenesis; histogenesis; organogenesis; synaptogenesis; gangliogenesis; embryogenesis; axonogenesis; retinogenesis; gliogenesis; glial progenitor cells; oligodendrocyte progenitor cells; retinal progenitor cells; nerve growth factor; neurotrophic factors; trophic factors; growth factors; neural tube defects; anencephaly; spina bifida; neuroblastoma cells; cell migration; neurogenesis; development; developmental stages

神经外胚层;神经脊;神经板;底板;神经管;轴索;神经胚形成;成神经细胞;干细胞;分化;个体发生;形态发生;组织发生;器官发生;突触发生;gangliogenesis;胚胎发生;axonogenesis;retinogenesis;gliogenesis;神经胶质祖细胞;少突神经胶质细胞祖细胞;视网膜祖细胞;神经生长因子;神经营养因子;营养因子;生长因素;神经管故障;无脑;脊柱裂;成神经细胞瘤细胞;细胞迁移;神经发生;发展;发展进程下一页末页共有 23 条记录,本页从第 1 到第 10 条。

All the indexes were tested in 2 -. 4 .. 6 weeks of operative intervals respectively. Results:(1) The general observation in the experimental group ,such as spirit, hair, body weight and activity ,was superior to that in the control group.(2) The recovery rate of sciatic nerve functional index (SF1) was statistically significantly higher in the experimental group than in the control group .(3) electrophysciological indexes: m the experimental group ,the tardy rate of induced motorius action potential ,recovery rates of the amplitude of compound muscle action potential and twitch tension and tetanic tension of triceps surae muscle ,were significantly higher than in the control group.(4) Compared with the control group ,the triceps surae muscle wet weight was significantly greater in the experimental group.(5) histomorphological indexes: the count of myelinated nerve fibers ,diameter of regenerated axon, thickness of nerve myelin sheath and area of capillary were much more in the experimental group than in the control group.(6) observation of ultrastructure with the transmission election microscope: more abundant organell a and maturer myelin sheath lay in the injured peripheral nerve of the experimental group, while not lay in the control group.

分别在木后第2、4、6周时进行各项指标的检测,结果发现:(1)实验组SD大鼠在整个实验过程中,其精神、毛发、体重,运动灵活性等一般情况优于对照组;(2)实验组的坐骨神经功能指数恢复率显著优于对照组,P<0.01;(3)神经电生理指标:实验组运动神经诱发电位潜伏期的延迟率显著优于对照组,P<0.05;复合肌肉动作电位振幅的恢复率显著高于对照组,两组比较第2周时P<0.01,第4、6周时,P<0.05;小腿三头肌单收缩力和强直收缩力的恢复率实验组显著高于对照组,P<0.01;(4)小腿三头肌湿重的恢复率,第2、4周时实验组高于对照组,差异非常显著,P<0.01;第6周时,差异仍显著,P<0.05;(5)组织形态学指标:有髓种经轴突计数、再生轴突直径的恢复率,毛细血管面积,实验组均显著大于对照组,P<0.01、P<0.05、P<0.001;实验组髓鞘厚度的恢复率也显著高于对照组,第2周时P<0.01,第4、6周时,P<0.05;(6)透射电镜超微结构观察:实验组再生轴突中细胞器丰富,髓鞘结构成熟,神经再生情况优于对照纽。

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