神经营养的

Research concerning research status and development of CNTF was selected, including changes of CNTF following nerve injury; the repair mechanism and protection of CNTF on neurons; regulation on reparative process; the recovery of axoplasmic transport function of axoplasm, as well as the regeneration on injured nerves.

纳入与神经损伤与睫状神经营养因子研究现状与发展密切相关的研究，包括神经元损伤后的变化；睫状神经营养因子对神经元的修复机制及保护作用，对修复过程的调控，对神经元轴浆运输功能的恢复，对损伤神经的再生作用。

Factor of 1 nerve nutrition and lack gene of nutrition of ill nerve of blood-vessel of head of courage and uprightness is a group of special protein and peptide kind element, this are familial include nerve to grow factor (factor of nutrition of nerve of sex of NGF), head source , nerve - 3, alkalescent grow into fiber cell factor and nerve of sex of colloid cell source.

现将几种重要的非炎性细胞因子和缺血性脑血管病关系的探究进展作以下综述。1神经营养因子和缺血性脑血管病神经营养因子是一组非凡的蛋白质和肽类分子，该家族包括神经生长因子、脑源性神经营养因子、神经营养素-3、碱性成纤维细胞生长因子和胶质细胞源性神经营养因子等。

The technology of culture of exogenetic NGF in vitro is qualified now, however, the exogenetic NGF cant get through blood-brain barrier and blood-nerve barrier because it is a kind of marcomolecular protein, thus its extensive application on clinic is limited, now, many scholors have turned their study direction to enhancing the content of endogenous NGF.

外源性NGF的体外合成技术已基本成熟，然而，外源性神经营养因子作为一种高分子蛋白物质不能通过消化道给药、不能通过血脑屏障和血神经屏障的问题阻碍了它在临床上的广泛应用，所以NGF在临床上并没有真正发挥出其营养神经的作用。

Research of microenvironment werefocused on the neurotrophic factors. Many studies have been done to use nerve conduits filled with exogenetic NTFs with the intention of promote axonal regeneration to the greatest extent efficiently. However concentration of exogenetic NTFs and the means of releasing NTFs still need to be studied.

对于后者，研究主要集中在神经营养因子(neurotrophic factors，NTFs)，众多学者尝试将外源性NTFs引入神经导管，以期尽可能有效地促进轴突再生，但外源性NTFs导入的浓度和释放的途径仍需进一步研究，同时最有益于神经再生所必须的营养因子的数量和种类也待进一步明确。

The experimental study is to explore the effect about rhEPO on neuromotor function, secondary pathology change and expression of NTF and the apoptosis of neural cell, which administered at different time after traumatic spinal cord injury. The experimental study is devided into three parts: The first part The effect of rhEPO on NT-3 and NGF expression after traumatic spinalcord injury Objective Nerve growth factor and neurotrophic factor–3(NT-3) are chief members in neurotrophic factors family, which can promote nerve cell survival、growth and differentiation.

本研究拟在脊髓损伤后不同时间应用促红细胞生成素干预，探讨其对神经运动功能和继发性病理改变的保护作用及对神经营养因子表达和神经细胞凋亡的影响，共分为以下三个部分：第一部分脊髓损伤后促红细胞生成素对NGF、NT-3表达的影响目的：神经生长因子和神经营养因子-3(NT-3)是神经营养因子家族的主要成员，是机体产生的能够促进神经细胞存活、生长、分化的一类多肽或蛋白质因子。

This paper reviewed the effect of some major neurotrophic factors on diabetic neuropathy and their application in clinical treatment.

本文对一些主要的神经营养因子对糖尿病性神经病理变性及再生作用，以及神经营养因子在临床糖尿病性神经变性的应用研究作－综述。

Cells were collected to undergo HE staining and immunocytochemistry reaction against Thy-1 to identify RGCs. Axons length of retinal neurons and RGCs were measured by microruler under microscope respectively to evaluate the neurotropic effects of B vitamins in improving the regeneration and growth of axons for retinal neurons and RGCs. Finally, we observed the reaction of retinal neurons when exposed to the insult of glucose deprivation. The neuroprotection of B vitamins were also evaluated at the same time.

对B族维生素的神经营养和保护作用的研究过程分为三个阶段，首先确定维生素B族对视网膜神经元是否有神经营养作用，并根据细胞活力找出最佳有效作用浓度；然后在接种细胞的即刻用各种维生素的最佳有效作用浓度作用于视网膜神经元，并在不同时间终止培养，收集细胞，HE染色和RGCs的免疫细胞化学染色，分别测量视网膜神经元和RGCs的轴突长度，评价维生素B族是否有促进视网膜神经元和RGCs轴突再生伸长的神经营养作用；最后，观察视网膜神经元对低糖损伤的反应，及维生素B族对受损的细胞是否有保护作用。

They are already being evaluated in clinical trials as potential therapeutic agents for major human neurodegenerative diseases, such as Alzheimer's disease, Parkinson's disease and motor neuron disease. However, delivery of polypeptide growth factors to compromised neurons in the CNS is problematic because their plasmatic half-life are very short and their abilities to cross the blood-brain barrier are theoretically null.

然而由于中枢神经系统中存在血脑屏障，神经营养因子类物质难以通过血脑屏障而到达靶部位；同时又因为神经营养因子在体内生物半衰期较短，使得全身性用药效果不够理想，另外还往往产生意想不到的副作用，如何解决这些问题对于深入研究神经营养因子在神经损伤及神经系统退行性疾病中的作用及开展临床应用前景研究有着十分重要的意义。

Methods: Dorsal root ganglions were derived from the chick embryo and dispersed into single neuron cell,The rhCNTF was added to neuron cells and incubated for 64 hours,The activity of acid phosphatase in neuron cells was determined and the biological activity of rhCNTF was analyzed quantificationally.

从鸡胚中分离出背根神经节并制成神经细胞，将重组人睫状神经营养因子加入到细胞中继续培养64h后，用酸性磷酸酶法检测活细胞内酸性磷酸酶的活性，从而定量测定重组人睫状神经营养因子的生物活性。

Methods The rat MSCs were isolated primarily from the femurs and tibias of the Wistar rats. MSCs were cultured, proliferated and purified by passage culture. After being induced by bFGF, cultuered MSCs were divided into experimental groups (GM1 group, GDNF group and GDNF+ GM1 group) and control group.

取雄性Wistar大鼠股骨和胫骨骨髓，进行MSCs的体外培养、传代扩增及纯化。bFGF预诱导24 h后，依据加入的神经营养因子不同分为单唾液酸四己糖神经节苷脂（GM1）组、胶质源性神经营养因子组和GDNF+GM1组，以及对照组。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。