神经营养

First neurotrophic factors could be concentrated within nerve conduits. Second fibre-bridge to anastomose two parts of nerve gaps could be formed ecxactly. Third operation of nerve conduits could be transplanted easily.

神经导管一方面能使神经营养因子在导管内积聚，浓度增高，另一方面引导神经缺损两端准确对合，同时导管的植入操作简单，可以减少手术对神经的损伤。

Objective To study the effects of olfactory ensheathing cells , NGF and BDNF on the proliferation of neural stem cells.

目的 研究嗅鞘细胞及其表达的神经生长因子和脑源性神经营养因子对神经干细胞增殖的影响。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Cortis tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL／6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像；②获得了各种听功能密切相关的结构的成熟时间点，基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14)；③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点：④Calbindin在E16以后的螺旋神经元没有表达，但是在scarpa神经元持续表达。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Corti\'s tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL／6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像；②获得了各种听功能密切相关的结构的成熟时间点，基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14)；③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点：④Calbindin在E16以后的螺旋神经元没有表达，但是在scarpa神经元持续表达。

Objective To study the levels of brain-derived neurotrophic factor protein in experimental dogs using multiple cauda equina constrictions throughout the entire lumbar, sacral and coccygeal spinal cord and their central processes of the dorsal root ganglia neurons resulting in the acute and severe cauda equina syndrome.

目的研究实验性犬急性重度马尾神经压迫48h的相关脊髓和背根神经节（dorsal root ganglion，DRG）内脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）蛋白表达水平。

Part 4: Brain-derived neurotropic factor genetically modified olfactory ensheathing cells of the neonatal rat Objective To culture the olfactory ensheathing cells from the neonatal rat and modify them genetically by human Brain-derived neurotropic factor to increase the formation of neurophic factors. Methods The OECs were dissociated from the neonatal rat olfactory bulbs and purified with time-speed difference stick.

第四部分：脑源性神经营养因子基因转染新生大鼠嗅鞘细胞目的为提高嗅鞘细胞(Olfactory ensheathing cells，OECs)分泌脑源性神经营养因子(BDNF，Brain-derived neurotropic factor)的能力，探讨将外源性BDNF基因转染入OECs，为今后采用BDNF基因修饰的OECs移植治疗SCI奠定基础。

There is plenty of polyphyly anastomosis of sural nerve's nutrient vessels and there will be better for offering the flood through the sural nerve's nutrient vessels design retrograde skin flap.

腓肠神经营养血管有多源性、吻合丰富的特点，以腓肠神经营养血管设计逆行皮瓣，血供较好，修复足部软组织缺损能获得较好效果。

There is plenty of polyphyly anastomosis of sural nerve's nutrient vessels and there will be better for offering the flood through the sural nerve's nutrient vessels design retrograde skin flap. We can get a better result through the way of repairing the lack of foot soft tissue.

腓肠神经营养血管有源性、吻合丰富的特点，以腓肠神经营养血管设计逆行皮瓣，血供较好，修复足部软组织缺损能获得较好效果。

Methods 12 patients with soft tissues defects around the foot of the diabetes were repaired by musculocutaneous,neurocutaneous axial island flaps transfer which included 4 cases of femoral forelateral flap,1 case of latissimus dorsi muscular flap,1 case of tensor fascia lata musculocutaneous flap,3 case of island flaps based on the sural nerve,1 case of island flaps based on the peroneus superficialis nerve and 2 cases of medial crural faee flap.

目的 探讨皮瓣移植修复糖尿病足组织缺损的临床效果方法采用股外侧皮瓣4例，背阔肌皮瓣1例，阔筋膜张肌皮瓣1例游离移植，腓肠神经营养血管蒂岛状皮瓣逆行转位3例，腓浅神经营养血管蒂岛状皮瓣逆行转位1例，小腿内侧皮瓣2例逆行移植修复糖尿病足组织缺损创面12例。

Methods 12 patients with soft tissues defects around the foot of the diabetes were repaired by musculocutaneous,neurocutaneous axial island flaps transfer which included 4 cases of femoral forelateral flap,1 case of latissimus dorsi muscular flap,1 case of tensor fascia lata musculocutaneous flap,3 case of island flaps based on the sural nerve,1 case of island flaps based on the peroneus superficialis nerve and 2 cases of medial crural faee flap.

采用股前外侧皮瓣4例，背阔肌皮瓣1例，阔筋膜张肌皮瓣1例游离移植，腓肠神经营养血管蒂岛状皮瓣逆行转位3例，腓浅神经营养血管蒂岛状皮瓣逆行转位1例，小腿内侧皮瓣2例逆行移植修复糖尿病足组织缺损创面12例。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。