神经膜细胞

Results: The numbers of Bombesin positive pulmonary cells, the lamina propria S-l00 protein, neuron-specific enolase positive nerve fibers, IgE positive cells, mast cells and IgE positive mast cells significantly increased in bronchiectasis. The changes of pulmonary endocrine cells, nerve fibers and IgE positive cells were more significantly in hyperplastic BALT areas. The S-100 and NSE were found in lymphoid tissue and BALT. A close contact was found between mast cells and the S-100 positive nerve fibers. An IgE positive outer zone was found on MC surface. Mast cells and IgE positive cells were seen in the bronchial epithelium and alveolar septa.

结果：支气管扩张症中，支气管上皮蛙皮素阳性细胞、固有膜S-100蛋白和神经特异性烯醇化酶阳性神经纤维、IgE阳性细胞、MC和IgE阳性MC均显著增多，且在支气管相关淋巴组织增生的区域上述肺内分泌细胞、神经纤维和IgE阳性细胞增多尤为显著，S-100蛋白和NSE阳性神经纤维分布於弥散淋巴组织和BALT中，MC与S-100蛋白阳性神经纤维紧密接触，MC表面有IgE阳性环状带，MC和IgE阳性细胞出现在支气管上皮间和肺泡壁。

The proximal and distal nerve stumps were sutured l cm apart, in an end-to-side fashion like ＂π＂, to the epneurium windows, opened by removing the epineural sheath on the intact tibial nerve trunk with 1 mm long.

方法将大鼠右侧腓总神经在胭窝处剪除约5mm后，将断裂的腓总神经近端和远端分别就近与同侧胫神经上的外膜窗施行端侧吻合，吻合口间距约10mm；动物存活24个月后，将手术侧近端吻合口以上胫神经剪断，再将辣根过氧化物酶注入远段腓总神经干内，72h后取材并经四甲基联苯胺显色显示脊髓L3-6节段神经元和L4、L5脊神经节细胞，同时取腓总神经远段行电镜观察神经纤维再生状态。

After disposal,schwann cells, myelin sheath and axon of nerve fibers were eliminated,but the basement membrane tube of schwann cells and three-dimensionalsupports of extracellular matrix of epineurium and perineurium werereserved.

经脱细胞处理后，去除了Schwann细胞、神经纤维的髓鞘和轴突，保存了由Schwann细胞基底膜管以及神经外膜和束膜的细胞外基质构成的三维支架结构。

Acellular Extracellular Matrixis three dimensional mesh stent which mainly composed by vessel of basilar membrane and coated by epineurium. As a bioactive nerve graft,it own natural construction,lower immunogenicity.

脱细胞神经细胞外基质材料是以SC基底膜管为主、以神经外膜为外套的完整的三维网状支架；它作为有生物活性的神经替代物，具有良好的仿生性，较低的免疫原性。

Large numbers of experiments internal and external had confirmed both direct current and square pulse of distal end cathodal could improve peripheral nerve regeneration, because it could speed up transmigration, creeper, growth and development of cell Schwann, derivate growth of nerve fiber; it also increased blood capillary of epineurium, brood blood vessel, amendment ischemic of impaired nerve, it still could accelerate Wallerian

国内外大量的基础实验及临床研究都证实远端负极电流的直流电场及脉冲电场等均有促进周围神经再生的作用，认为其可加速Schwann细胞的游走、爬行及生长发育，诱导神经纤维的生长；电刺激还能促使神经外膜毛细血管数量增多，扩张血管，改善神经损伤段缺血状态，并加速Wallerian变性及崩解组织的清除，为Schwann细胞发育和髓鞘化过程提供充足的能量与物质，同时为神经轴突的再生提供良好的内环境；在神经断端出现瘢痕或神经瘤时，电刺激可增加神经纤维穿越瘢痕的能力。

Methods: Study the newly found cell with the weak silver carbonate staining method of del Rio-Hortega. Using transmission electron microscopy, compare the newly found cell with the atypical cell found by Marc Lenoir and Philippe Vago, and extend young SD rat model to adult SD rat, young and adult Wistar rat,and extend drug of Neomycin to Amikacin to study the universality.

采用经典的小胶质细胞特殊染色方法——银染法(Hortega氏碳酸银法)对大鼠耳蜗药物损伤后出现的新细胞进行研究；通过透射电镜与Marc Lenoir和Philippe Vago实验动物模型中的"非典型细胞"进行了比较与扩展研究；运用逆转录PCR技术检测耳蜗基底膜上神经干细胞标志物nestin的表达情况，对新细胞的来源及其与神经干细胞或nestin的前体细胞的关系作进一步研究。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Cortis tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL／6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像；②获得了各种听功能密切相关的结构的成熟时间点，基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14)；③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点：④Calbindin在E16以后的螺旋神经元没有表达，但是在scarpa神经元持续表达。

Results①Make clear of the general principal during the morphologicaldevelopment of the C57BL/6 mouse inner ear and reconstructed the 3D modelof E13 inner ear;②The mature time of many histological structuresassociated closely with the hearing setout of C57BL/6 mouse were madeclear as basilar membrane in D10, scala vestibular and tympanicD4, Corti\'s tunnel D12, inner sulcus D10, tectorial membrane D10, haircells D12, spiral ganglion neurons D12 and neurofilament in cochlear axisD12-D14.③Outlined the time table and the patterns of the expressionsof those proteins associated with the inner ear development which includedproneural committed proteins, neuronal differential proteins,neurotrophic factors and neuron special proteins.

结果①HE常规染色大体观上了解了C57BL／6小鼠内耳形态发育E8-Da期间的一般规律并且获得了E13的三维立体图像；②获得了各种听功能密切相关的结构的成熟时间点，基底膜(D10)、鼓阶前庭阶(D4)、螺旋器隧道(D12)、内螺旋沟(D10)、盖膜(D10)、毛细胞(D12)、螺旋神经元(D12)和蜗轴纤维(D12-D14)；③完成了前神经决定蛋白、神经分化蛋白、神经营养因子和神经元特异性蛋白等内耳发育相关的蛋白的表达时限表并详细观察了其在内耳各个部位表达随发育变化的特点：④Calbindin在E16以后的螺旋神经元没有表达，但是在scarpa神经元持续表达。

Results Among the 52 cases, 30 cases were confirmed to fibrosarcomas, which were identical with primary dingnoses;22 cases were revised to malignant fibrous histocytoma (8 cases), malignant nerve sheath tumor (4 cases), monophasic spindle cell synovial sarcoma (3 cases), leiomyosarcoma(3 cases), rhabdomyosarcoma(2 cases) and abdominal desmmoid tumor (2 cases). Of the 18 cases, only two cases were misdiagnosed and were revised to malignant fibrous histocytoma and spindle cell synovial sarcoma, respectively.

结果：1980年前52例中维持原诊断30例，修改诊断22例(42.3%)，分别为恶性纤维组织细胞瘤8例、恶性神经鞘膜瘤4例、单相性梭形细胞滑膜肉瘤3例、平滑肌肉瘤3例、横纹肌肉瘤2例、韧带状纤维瘤2例；1980年后纤维肉瘤18例中，维持原诊断16例，修改诊断2例(11.1%)，分别为恶性纤维组织细胞瘤和滑膜肉瘤各1例。

Results showed that there were the morphological changes of mitochondria, such as vacuolization, degeneration and cristae swelling or disappearance, in HT-29 cells treated by C2-ceramide for 24 hours. Under the fluorescence microscope, mitochondria of apoptotic cells aggregated and emitted red fluorescence, however, mitochondria of normal cells emitted green fluorescence. The mitochondrial membrane potential began to decrease after 6 hours in HT-29 cells treated with C2-ceramide in time and dose dependent manner.

结果显示C2-神经酰胺作用HT-29细胞24小时，线粒体发生空泡化、变性、嵴肿胀或消失等形态学改变；此时荧光显微镜下可观察到线粒体聚集，发出红色荧光，而正常细胞线粒体发出绿色荧光。C2-神经酰胺作用细胞6小时，线粒体膜电位即开始降低，呈时间-剂量-效应关系。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新